Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Persistence of Human Pathogens in a Crop Grown from Sewage Sludge Treated Soil
Thesis (PhD (Water Utilisation))--University of Pretoria, 2006.
Saved in:
| Other Authors: | |
|---|---|
| Format: | Thesis |
| Published: |
University of Pretoria
2013
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613523633242112 |
|---|---|
| access_status_str | Open Access |
| author2 | Snyman, H.G. |
| author_browse | Snyman, H.G. |
| author_facet | Snyman, H.G. |
| collection | Thesis |
| dc_rights_str_mv | © 2005, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Thesis (PhD (Water Utilisation))--University of Pretoria, 2006. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/28268 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:37:30.383Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/28268 Persistence of Human Pathogens in a Crop Grown from Sewage Sludge Treated Soil Snyman, H.G. upetd@ais.up.ac.za Chale-Matsau, Jacobeth Raesibe Bettina Poverty Management practice Low metal sludge High metal sludge Risk assessment Salmonella E.coli Sewage sludge Pathogen Ascaris UCTD Thesis (PhD (Water Utilisation))--University of Pretoria, 2006. The advantages associated with the use of sewage sludge in agricultural land have motivated many countries to use sewage sludge for soil amendment purposes. South Africa’s deteriorated agricultural soil could benefit from this nutritional and cost effective product. However, the major shortcoming of sewage sludge is the presence of various pathogenic microorganisms. This raised concern amongst researchers with regard to public safety. The focus of this study, was to investigate the prevalence of pathogens in a crop grown in soil enriched with sewage sludge and to determine risk of infection thereof and to suggest appropriate management practice for sewage sludge use. Potato (Solanum tuberrosum), which is a high risk crop was used, to simulate a worst case scenario. Both the low metal sludge (LMS) and high metal sludge (HMS) were found to have associated diverse numbers of bacteria. Using culture-based technique, E.coli and Salmonella spp were found to persist in soil throughout the experimental period. One treatment option (LMS 16 tons/ha) showed a prevalence of these microorganisms in potatoes. Subsequent molecular studies based on amplification of 16S rRNA gene, yielded limited contamination of potatoes with enteric pathogens, however diverse types of opportunistic, pathogens (mostly environmental pathogens) were isolated from the potatoes. Enteric pathogens were isolated from the sewage treated soil in which these potatoes were grown. This study has indicated that growing even high risk crops, may lead to limited infestation of produce with primary pathogens. However, proper treatment of sewage sludge prior to use in agriculture is recommended to ensure public safety. The management requirements indicated in this study serve as recommended actions that can be implemented to ensure human safety with regard to sludge application to agricultural land. Chemical Engineering unrestricted 2013-09-07T13:12:52Z 2005-09-29 2013-09-07T13:12:52Z 2005-06-13 2006-09-29 2005-09-29 Thesis Chale-Matsau, J 2005, Persistence of Human Pathogens in a Crop Grown from Sewage Sludge Treated Soil, PhD thesis, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/28268 > http://hdl.handle.net/2263/28268 http://upetd.up.ac.za/thesis/available/etd-09292005-114114/ © 2005, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf University of Pretoria |
| spellingShingle | Poverty Management practice Low metal sludge High metal sludge Risk assessment Salmonella E.coli Sewage sludge Pathogen Ascaris UCTD Persistence of Human Pathogens in a Crop Grown from Sewage Sludge Treated Soil |
| title | Persistence of Human Pathogens in a Crop Grown from Sewage Sludge Treated Soil |
| title_full | Persistence of Human Pathogens in a Crop Grown from Sewage Sludge Treated Soil |
| title_fullStr | Persistence of Human Pathogens in a Crop Grown from Sewage Sludge Treated Soil |
| title_full_unstemmed | Persistence of Human Pathogens in a Crop Grown from Sewage Sludge Treated Soil |
| title_short | Persistence of Human Pathogens in a Crop Grown from Sewage Sludge Treated Soil |
| title_sort | persistence of human pathogens in a crop grown from sewage sludge treated soil |
| topic | Poverty Management practice Low metal sludge High metal sludge Risk assessment Salmonella E.coli Sewage sludge Pathogen Ascaris UCTD |
| url | http://hdl.handle.net/2263/28268 http://upetd.up.ac.za/thesis/available/etd-09292005-114114/ |