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Genetic variation of Kappa-casein in South African goats

Dissertation (MSc(Agric))--University of Pretoria, 2009.

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Other Authors: Van Marle-Koster, Este
Format: Thesis
Published: University of Pretoria 2013
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access_status_str Open Access
author2 Van Marle-Koster, Este
author_browse Van Marle-Koster, Este
author_facet Van Marle-Koster, Este
collection Thesis
dc_rights_str_mv © 2009, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.
description Dissertation (MSc(Agric))--University of Pretoria, 2009.
format Thesis
id oai:repository.up.ac.za:2263/28891
institution University of Pretoria (South Africa)
last_indexed 2026-06-10T12:40:46.593Z
license_str Other — see source repository
provenance_str_mv Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository
publishDate 2013
publishDateRange 2013
publishDateSort 2013
publisher University of Pretoria
publisherStr University of Pretoria
record_format dspace
source_str UPSpace — University of Pretoria Institutional Repository
spelling oai:repository.up.ac.za:2263/28891 Genetic variation of Kappa-casein in South African goats Van Marle-Koster, Este Visser, Carina upetd@up.ac.za Scheepers, Robyn Clair Milk quantity South african goats Kappa-casein UCTD Dissertation (MSc(Agric))--University of Pretoria, 2009. Milk protein polymorphisms have a significant influence on milk quantity and composition. Kappa-casein is of special interest due to its known relationship with milk quality. In goats, a number of allelic variants have been identified, primarily classified into two groups. Group BIEF alleles (D, E, K, and M) have been shown to have a positive effect on milk yield and technological properties, while group AIEF, the remaining alleles, have a less positive influence on milk composition. The aim of this study was to investigate genetic variation in the kappa-casein genotype of South African goats. PCR-RFLP and DNA sequencing were performed on 68 and 77 samples, respectively. In addition, 84 milk samples were analyzed for milk composition. RFLP analysis revealed that the A and/or B alleles were the most frequent in the populations studied. A frequency of 0.00 was observed for the BIEF variants using DNA sequencing. In all goat types included, the B allele was the most common, with frequencies ranging from 60% in SA Boer goats to 100% in Saanens. The B’ allele had lower frequencies of 0.357 and 0.207 in SA Boer goats and local goat types, respectively. The H allele was present at low frequencies in local goat types (10.3%) and in SA Boer goats (3.6%), but was absent in Saanens. AMOVA results indicated that most of the total variation occurred within populations (80.66%) with the remainder of the variation (FST = 0.1934; p < 0.01) occurring due to genetic differences between populations. Animal and Wildlife Sciences unrestricted 2013-09-07T14:26:07Z 2009-11-03 2013-09-07T14:26:07Z 2009-10-21 2009 2009-10-21 Dissertation Scheepers, RC 2009, Genetic variation of Kappa-casein in South African goats, MSc(Agric) dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/28891 > E1524/gm http://hdl.handle.net/2263/28891 http://upetd.up.ac.za/thesis/available/etd-10212009-143858/ © 2009, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria
spellingShingle Milk quantity
South african goats
Kappa-casein
UCTD
Genetic variation of Kappa-casein in South African goats
title Genetic variation of Kappa-casein in South African goats
title_full Genetic variation of Kappa-casein in South African goats
title_fullStr Genetic variation of Kappa-casein in South African goats
title_full_unstemmed Genetic variation of Kappa-casein in South African goats
title_short Genetic variation of Kappa-casein in South African goats
title_sort genetic variation of kappa casein in south african goats
topic Milk quantity
South african goats
Kappa-casein
UCTD
url http://hdl.handle.net/2263/28891
http://upetd.up.ac.za/thesis/available/etd-10212009-143858/