Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Identification of Leptographium species by oligonucleotide discrimination on a DNA microarray
Dissertation (MSc)--University of Pretoria, 2009.
Saved in:
| Other Authors: | |
|---|---|
| Format: | Thesis |
| Published: |
University of Pretoria
2013
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613625014812672 |
|---|---|
| access_status_str | Open Access |
| author2 | Wingfield, Brenda D. |
| author_browse | Wingfield, Brenda D. |
| author_facet | Wingfield, Brenda D. |
| collection | Thesis |
| dc_rights_str_mv | © 2009, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2009. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/28928 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:39:06.697Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/28928 Identification of Leptographium species by oligonucleotide discrimination on a DNA microarray Wingfield, Brenda D. Wingfield, Michael J. Jacobs, Karin Van Zuydam, Natalie Rachel Leptographium Microarray Species identification UCTD Dissertation (MSc)--University of Pretoria, 2009. Leptographium is an anamorph genus within the Ophiostomatoid group of fungi and represents a unique case for molecular applications. The genus has a near complete sequence data available for three genes across all known species. This characteristic makes it a perfect test group for investigating applications of new diagnostic techniques within ascomycetes. Probes and primers, for microarrays, are designed from phylogenetically useful gene regions and are fabricated onto a solid substrate using printing technology. The sample is prepared using PCR and is hybridised to the probes under stringent conditions. The resulting fluorescent pattern is rigorously analysed to distinguish species from each other. Diagnostic PCR uses primers that are designed in similar way to the way probes are designed for microarrays and indicate the presence of a species through positive amplification. This research methodology will be applied to Leptographium to evaluate the efficacy of microarray technology for discriminating species within that genus. The data gained from this research study will be used in applications for other genera using microarray technology. Genetics Unrestricted 2013-09-07T14:30:53Z 2009-11-03 2013-09-07T14:30:53Z 2009-09-02 2009 2009-10-22 Dissertation Van Zuydum, N 2009, Identification of Leptographium species by oligonucleotide discrimination on a DNA microarray, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/28928 > E1490/gm http://hdl.handle.net/2263/28928 http://upetd.up.ac.za/thesis/available/etd-10222009-102934/ © 2009, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Leptographium Microarray Species identification UCTD Identification of Leptographium species by oligonucleotide discrimination on a DNA microarray |
| title | Identification of Leptographium species by oligonucleotide discrimination on a DNA microarray |
| title_full | Identification of Leptographium species by oligonucleotide discrimination on a DNA microarray |
| title_fullStr | Identification of Leptographium species by oligonucleotide discrimination on a DNA microarray |
| title_full_unstemmed | Identification of Leptographium species by oligonucleotide discrimination on a DNA microarray |
| title_short | Identification of Leptographium species by oligonucleotide discrimination on a DNA microarray |
| title_sort | identification of leptographium species by oligonucleotide discrimination on a dna microarray |
| topic | Leptographium Microarray Species identification UCTD |
| url | http://hdl.handle.net/2263/28928 http://upetd.up.ac.za/thesis/available/etd-10222009-102934/ |