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In vitro antimalarial activity of ethnobotanically selected indigenous plants and characterisation of a bioactive compound
Dissertation (MSc (Plant Physiology))--University of Pretoria, 2008.
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University of Pretoria
2013
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| _version_ | 1867613635808854016 |
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| access_status_str | Open Access |
| author2 | Meyer, J.J.M. (Jacobus Johannes Marion) |
| author_browse | Meyer, J.J.M. (Jacobus Johannes Marion) |
| author_facet | Meyer, J.J.M. (Jacobus Johannes Marion) |
| collection | Thesis |
| dc_rights_str_mv | © 1999 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Plant Physiology))--University of Pretoria, 2008. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/29226 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:39:17.410Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/29226 In vitro antimalarial activity of ethnobotanically selected indigenous plants and characterisation of a bioactive compound Meyer, J.J.M. (Jacobus Johannes Marion) Louw, Abraham Izak upetd@up.ac.za Prozesky, Erwin Antoni Floral resources Antimalarial drugs Ethnobotanical Malaria UCTD Dissertation (MSc (Plant Physiology))--University of Pretoria, 2008. Malaria still remains one of the world’s biggest killers with more than two million people dying from the disease each year. Present drugs have become ineffective because parasites are developing resistance to most of them. Efforts are now being directed in obtaining drugs with different structural features. Plants have provided most of the antimalarial drugs so far and efforts now concentrate on screening plants for new antimalarial drugs. South Africa with its rich floral resources and ethnobotanical history is an ideal place to screen plants for antimalarial activity. The antimalarial activity of 20 extracts from 14 ethnobotanically selected South African plants was screened for antimalarial activity in vitro. Results obtained showed that most of the plants had strong antimalarial activity. IC50 values obtained with the flow cytometric method were between 0.9 and 2 µg/ml for 9 of the 10 selected extracts. This represents a very high number of extracts with very good antimalarial activity. Cytotoxicity of the most active extracts were determined against monkey kidney cells as well as a luminescent bacteria method. Results obtained had a ID50 between 35 and 100 µg/ml with the monkey kidney cell test and between 100 and 2000 µg/ml with the bacteria test. Therapeutic values ranged between 35 and 100. Extracts therefore have a poor selectivity towards Plasmodium. The dichloromethane extract from Ozoroa engleri was further purified with silica gel column chromatography, Sephadex column chromatography and HPLC. Results obtained showed at least five or six compounds responsible for the antimalarial activity of the extract, all with moderate antimalarial activity and no further efforts were undertaken to identify them. The acetone extract of Croton pseudopulchellus was then selected for isolation of active principles and was purified by silica gel column chromatography, Sephadex column chromatography and PTLC. Kaurenoic acid was isolated as one of the active principles and identified by NMR and GC-MS. Kaurenoic acid was found to have an antimalarial IC50 of 38 µg/ml, while its cytotoxicity ID50 was 35 µg/ml. Kaurenoic acid was responsible for only some of the activity found in the purified fraction and other compound(s) in the extract might have much better antimalarial activity. Plant Science unrestricted 2013-09-07T15:10:52Z 2008-11-11 2013-09-07T15:10:52Z 1999-04-23 2008-11-11 2008-11-04 Dissertation Prozesky, EA 1999, In vitro antimalarial activity of ethnobotanically selected indigenous plants and characterization of a bioactive compound, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/29226 > H1091/ag http://hdl.handle.net/2263/29226 http://upetd.up.ac.za/thesis/available/etd-11042008-085442/ © 1999 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Floral resources Antimalarial drugs Ethnobotanical Malaria UCTD In vitro antimalarial activity of ethnobotanically selected indigenous plants and characterisation of a bioactive compound |
| title | In vitro antimalarial activity of ethnobotanically selected indigenous plants and characterisation of a bioactive compound |
| title_full | In vitro antimalarial activity of ethnobotanically selected indigenous plants and characterisation of a bioactive compound |
| title_fullStr | In vitro antimalarial activity of ethnobotanically selected indigenous plants and characterisation of a bioactive compound |
| title_full_unstemmed | In vitro antimalarial activity of ethnobotanically selected indigenous plants and characterisation of a bioactive compound |
| title_short | In vitro antimalarial activity of ethnobotanically selected indigenous plants and characterisation of a bioactive compound |
| title_sort | in vitro antimalarial activity of ethnobotanically selected indigenous plants and characterisation of a bioactive compound |
| topic | Floral resources Antimalarial drugs Ethnobotanical Malaria UCTD |
| url | http://hdl.handle.net/2263/29226 http://upetd.up.ac.za/thesis/available/etd-11042008-085442/ |