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Bacterial endophytes associated with Eucalyptus nitens clones
Dissertation (MSc)--University of Pretoria, 2012.
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| Format: | Thesis |
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University of Pretoria
2013
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| _version_ | 1867613570825453568 |
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| access_status_str | Open Access |
| author2 | Coutinho, Teresa A. |
| author_browse | Coutinho, Teresa A. |
| author_facet | Coutinho, Teresa A. |
| collection | Thesis |
| dc_rights_str_mv | © 2011, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria |
| description | Dissertation (MSc)--University of Pretoria, 2012. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/29299 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:38:15.015Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/29299 Bacterial endophytes associated with Eucalyptus nitens clones Coutinho, Teresa A. Venter, S.N. (Stephanus Nicolaas) angels@aspirata.co.za Stewart, Annie Cecilia Eucalyptus nitens clones UCTD Dissertation (MSc)--University of Pretoria, 2012. Plants are colonised by a vast amount of bacteria which are found in parts such as seeds, roots, leaves and fruits while fewer are found on blossoms, stems and vascular tissue. These different parts of plants make up distinct micro ecosystems which may result in different bacterial species (endophytes) colonizing these ecosystems. Such interactions could be for life or only a short period of time and may cause no significant damage or they could be latent pathogens. Isolations of both Gram negative and Gram positive bacteria have been made from an extensive range of plant species and include bacterial genera from the following groups: Firmicutes, Actinobacteria, á Proteobacteria, â Proteobacteria, and ã Proteobacteria. The focus of this study was the endophytic bacterial community of resistant, healthy and diseased Eucalyptus nitens clones, the latter of which showed symptoms of bacterial blight and die back previously described as caused by Pantoea ananatis. The endophytic bacteria of these sampled clones were studied using culturing dependent and independent methods. The focus was on the Enterobacteriaceae in order to determine whether P.ananatis is present as an endophyte of these clones. To obtain the isolates, standard culturing techniques were used, followed by sequence identification of the 16S rRNA as well as two housekeeping genes, rpoB and gyrB. Results obtained from the culturing study were compared to results obtained from a PCR-DGGE study of the same samples. Although no conclusion could be drawn as to which organism present caused the disease symptoms on the susceptible clones, it was seen that Enterobacter and Pantoea, were the most frequently isolated in both of the studies from all clones sampled. This implies that they are present as endophytes in the E.nitens clones, together with Pseudomonas and Bacillus as suggested by the DGGE study. Copyright Microbiology and Plant Pathology unrestricted 2013-09-07T15:18:10Z 2012-11-12 2013-09-07T15:18:10Z 2012-09-07 2012-11-12 2012-11-07 Dissertation Stewart, AC 2011, Bacterial endophytes associated with Eucalyptus nitens clones, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/29299 > E12/9/56/gm http://hdl.handle.net/2263/29299 http://upetd.up.ac.za/thesis/available/etd-11072012-133714/ © 2011, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria application/pdf University of Pretoria |
| spellingShingle | Eucalyptus nitens clones UCTD Bacterial endophytes associated with Eucalyptus nitens clones |
| title | Bacterial endophytes associated with Eucalyptus nitens clones |
| title_full | Bacterial endophytes associated with Eucalyptus nitens clones |
| title_fullStr | Bacterial endophytes associated with Eucalyptus nitens clones |
| title_full_unstemmed | Bacterial endophytes associated with Eucalyptus nitens clones |
| title_short | Bacterial endophytes associated with Eucalyptus nitens clones |
| title_sort | bacterial endophytes associated with eucalyptus nitens clones |
| topic | Eucalyptus nitens clones UCTD |
| url | http://hdl.handle.net/2263/29299 http://upetd.up.ac.za/thesis/available/etd-11072012-133714/ |