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Identification of volatile organic compounds from Eucalyptus detected by Gonipterus scutellatus (Gyllenhal) females
Dissertation (MSc)--University of Pretoria, 2010.
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University of Pretoria
2013
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| _version_ | 1867613503553011712 |
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| access_status_str | Open Access |
| author2 | Rohwer, Egmont Richard |
| author_browse | Rohwer, Egmont Richard |
| author_facet | Rohwer, Egmont Richard |
| collection | Thesis |
| dc_rights_str_mv | © 2010 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2010. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/29414 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:37:11.117Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/29414 Identification of volatile organic compounds from Eucalyptus detected by Gonipterus scutellatus (Gyllenhal) females Rohwer, Egmont Richard mark.bouwer@fabi.up.ac.za Slippers, Bernard Bouwer, Marc Clement Snout beetle gonipterus scutellatus Eucalyptus globulus e. viminalis Non-host e. citriodora Volatile organic compounds (VOCs) UCTD Dissertation (MSc)--University of Pretoria, 2010. This thesis concerns the development of semiochemical identification expertise and methodology at the University of Pretoria. The Eucalyptus snout beetle Gonipterus scutellatus was used as a model insect in developing these methods, firstly because it is a known pest in the Eucalyptus forestry industry of South Africa. Secondly, nothing is known about its chemical ecology and lastly, it is a relatively large insect that is easily worked on. Three main techniques were used namely: Electroantennography (EAG), Gas Chromatography Electroantennography Detection (GC-EAD) and Gas Chromatog- raphy Mass Spectrometry (GC-MS). EAG was used to difierentiate and identify certain Eucalyptus species that were expected to contain compounds that may function as either kairomones or allomones for G. scutellatus. The EAG process revealed that G. scutellatus responds more intensely to damaged Eucalyptus leaves as compared to undamaged leaves. The crushed foliage of the known hosts Eucalyptus globulus and E. viminalis gave larger responses than the crushed foliage from a known non-host E. citriodora. We sampled the volatiles from the crushed foliage of these three species and tentatively identified sixteen compounds from the E. globulus volatile profile that was antennally active for G. scutellatus females. The presence of these volatiles were subsequently investigated for E. viminalis and E. citriodora. The green leaf volatiles, (Z)-3 hexenyl acetate, (Z)-3-hexen-1-ol and (E)-2-hexenal and aromatic compounds, 2-phenylethanol, benzyl acetate and ethylphenylacetate often gave larger responses than the terpenes such as α-pinene, β-pinene and camphene. Crushed E. globulus leaves contained 2-phenyl ethanol, benzyl acetate, ethylphenylacetate, eucalyptol, α-pinene, (Z)-3 hexenyl acetate, (Z)-3-hexen-1-ol and (E)-2-hexenal that were antennally active. The E. viminalis profile had very little 2-phenylethanol and virtually no benzyl acetate. The E. citriodora volatile profile contained very little (Z)-3-hexen-1-ol, (E)-2-hexenal, 2-phenylethanol, benzyl acetate and ethylphenylacetate. These compounds may influence the host selection behaviour of G. scutellatusfemales. These volatiles can be tested in a behavioural bioassay in order to determine their effect on the Eucalyptus snout beetle G. scutellatus. Chemistry unrestricted 2013-09-07T15:36:43Z 2010-11-11 2013-09-07T15:36:43Z 2010-09-02 2010-11-11 2010-11-11 Dissertation Bouwer, MC 2010, Identification of volatile organic compounds from Eucalyptus detected by Gonipterus scutellatus (Gyllenhal) females, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/29414 > E10/739/ag http://hdl.handle.net/2263/29414 http://upetd.up.ac.za/thesis/available/etd-11112010-152730/ © 2010 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Snout beetle gonipterus scutellatus Eucalyptus globulus e. viminalis Non-host e. citriodora Volatile organic compounds (VOCs) UCTD Identification of volatile organic compounds from Eucalyptus detected by Gonipterus scutellatus (Gyllenhal) females |
| title | Identification of volatile organic compounds from Eucalyptus detected by Gonipterus scutellatus (Gyllenhal) females |
| title_full | Identification of volatile organic compounds from Eucalyptus detected by Gonipterus scutellatus (Gyllenhal) females |
| title_fullStr | Identification of volatile organic compounds from Eucalyptus detected by Gonipterus scutellatus (Gyllenhal) females |
| title_full_unstemmed | Identification of volatile organic compounds from Eucalyptus detected by Gonipterus scutellatus (Gyllenhal) females |
| title_short | Identification of volatile organic compounds from Eucalyptus detected by Gonipterus scutellatus (Gyllenhal) females |
| title_sort | identification of volatile organic compounds from eucalyptus detected by gonipterus scutellatus gyllenhal females |
| topic | Snout beetle gonipterus scutellatus Eucalyptus globulus e. viminalis Non-host e. citriodora Volatile organic compounds (VOCs) UCTD |
| url | http://hdl.handle.net/2263/29414 http://upetd.up.ac.za/thesis/available/etd-11112010-152730/ |