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Functional and structural properties of an in silico model of Plasmodium falciparum spermidine synthase
Dissertation (MSc (Biochemistry))--University of Pretoria, 2005.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2013
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| _version_ | 1867613537656897536 |
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| access_status_str | Open Access |
| author2 | Louw, Abraham Izak |
| author_browse | Louw, Abraham Izak |
| author_facet | Louw, Abraham Izak |
| collection | Thesis |
| dc_rights_str_mv | © 2005, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Biochemistry))--University of Pretoria, 2005. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/30651 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:37:43.693Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/30651 Functional and structural properties of an in silico model of Plasmodium falciparum spermidine synthase Louw, Abraham Izak pieter.burger@tuks.co.za Prof F Joubert Joubert, Fourie Burger, Pieter Buys No key words available Dissertation (MSc (Biochemistry))--University of Pretoria, 2005. Half of the world’s population live in 103 countries plagued by malaria and more than a million people die annually of this devastating disease. Between 300 and 500 million clinical cases presents itself annually in these countries. 90% of the world’s malaria occurs in sub-Saharan Africa and most infections are due to P. falciparum, the most virulent human malaria parasite. The poorest nations are most at risk and malaria is estimated to cost these already poverty stricken countries an estimated US$12 billion annually. The increase in drug resistance, especially multiple drug resistance, is of great concern and highlights the pressing need for new anti-malarials. Rational drug design is a method where a 3D structure is used to assist in the design of new drugs. Since malaria proteins are difficult to express in high enough quantities to be crystallized, there are only a few malarial protein structures available that can be used in such studies. The use of computationally derived models provides an alternative to this limitation. In this study a computational approach was taken to gain further insight into the structure of PfSpmdSyn. The homology model of PfSpmdSyn was constructed from SpmdSyn of T. maritime and A. thaliana. The interactions between PfSpmdSyn and its two substrates, putrescine and dcAdoMet could not be obtained from docking studies and had to be derived from the substrate analogue AdoDATO. The PfSpmdSyn model was further refined by subjecting it to molecular dynamics to find the optimal interaction between the protein and substrates. The PfSpmdSyn homology models were partially validated by explaining mutational studies, which have been performed prior to the generation of the model. Additional mutational studies have been suggested to further validate the PfSpmdSyn homology model. Biochemistry Restricted Natural and Agricultural Sciences 2013-09-09T07:22:42Z 2006-03-02 2013-09-09T07:22:42Z 2005-05-08 2005-05 2006-03-02 Dissertation Burger, P 2005, Functional and structural properties of an in silico model of Plasmodium falciparum spermidine synthase, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://upetd.up.ac.za/thesis/available/etd-03022006-151329/ > http://hdl.handle.net/2263/30651 http://upetd.up.ac.za/thesis/available/etd-03022006-151329/ en © 2005, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf application/pdf application/pdf application/pdf University of Pretoria |
| spellingShingle | No key words available Functional and structural properties of an in silico model of Plasmodium falciparum spermidine synthase |
| title | Functional and structural properties of an in silico model of Plasmodium falciparum spermidine synthase |
| title_full | Functional and structural properties of an in silico model of Plasmodium falciparum spermidine synthase |
| title_fullStr | Functional and structural properties of an in silico model of Plasmodium falciparum spermidine synthase |
| title_full_unstemmed | Functional and structural properties of an in silico model of Plasmodium falciparum spermidine synthase |
| title_short | Functional and structural properties of an in silico model of Plasmodium falciparum spermidine synthase |
| title_sort | functional and structural properties of an in silico model of plasmodium falciparum spermidine synthase |
| topic | No key words available |
| url | http://hdl.handle.net/2263/30651 http://upetd.up.ac.za/thesis/available/etd-03022006-151329/ |