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The effect of florfenicol on egg hatchability in fowls
Dissertation (MSc)--University of Pretoria, 2012.
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| Format: | Thesis |
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University of Pretoria
2013
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| _version_ | 1867613680846241793 |
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| access_status_str | Open Access |
| author2 | Naidoo, Vinny |
| author_browse | Naidoo, Vinny |
| author_facet | Naidoo, Vinny |
| collection | Thesis |
| dc_rights_str_mv | © 2013 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria |
| description | Dissertation (MSc)--University of Pretoria, 2012. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/30951 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:40:00.333Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/30951 The effect of florfenicol on egg hatchability in fowls Naidoo, Vinny Al-Shahrani, Saeed Murie UCTD Dissertation (MSc)--University of Pretoria, 2012. While florfenicol is not registered for use in poultry, the product is used on a limited basis in broiler breeders for the treatment of E. coli by means of compounded solutions. While apparently efficacious, one unpublished adverse reaction report from the field suggest that florfenicol may interfere with embryogenesis of the developing egg. With the side effects of the product largely unknown in breeder birds, the aim of this study was to evaluate the effect of florfenicol on egg fertility in a fowl model. In this study 20 week old commercial layer breeder hens (n=30) and cockerels (n=4) in 4 groups were exposed to florfenicol in a phased manner, with the same groupings used for all phases. Prior to each phase, a wash-out period of three weeks was allowed. In phase 1, only the hens were treated with florfenicol at 0, 10, 20 and 30 mg/kg, respectively, while in phase II, only the cockerels were treated at doses of 0, 30, 60, 90 mg/kg. In phase III only hens again were treated at doses of 0, 30, 60, 90 mg/kg. In all phases, treatments were administered once daily for 5 days directly into the crop. Eggs were collected from all groups on days 0, 2, 4, and 5 of dosing and on days 1, 3, 4, 6, and 8 days post-treatment for incubation. Fertility was evaluated by candling, egg break-outs and number of chicks hatching. In phase III, five hens from each group were slaughtered at 0, 1, 2, 3 and 4 days after drug withdrawal to ascertain the lungs concentrations achieved for the florfenicol, while eggs (n=5) were collected on days 0, 1, 2, 3, and 4 for the same reason. Florfenicol was quantified in the tissues using a validated HPLC method. Doses of 0, 10, 20 or 30 mg/kg of florfenicol had no major effects on the embryo and hatchability. No fertility effects were noted when the cockerels were treated. However, the hens treated at 60 and 90 mg/kg showed signs of embryonic toxicity with a complete absence of hatchability (0%) being evident soon after treatment, which only returned to normal 5 days of treatment cessation. Florfenicol had no other overt toxic effects on the treated birds. The concentration of florfenicol in eggs at the dose of 90 mg/kg was 4.27 μgmg after five days of treatement. Based on the presence or absence of toxicity, the threshold egg concentration for toxicity appears to be 0.6 ug/mg. The safe period for the consumption of eggs after treatment was estimated to be 6 days. In conclusion, florfenicol is toxic to the embryo when the hens are treated with doses of 60 or 90 mg/kg for five consecutive days. Paraclinical Sciences MSc Unrestricted 2013-09-09T07:51:53Z 2013-06-28 2013-09-09T07:51:53Z 2013-04-12 2012 2013-06-21 Dissertation Al-Shahrani, SM 2012, The effect of florfenicol on egg hatchability in fowls, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/30951> E13/4/647/gm http://hdl.handle.net/2263/30951 http://upetd.up.ac.za/thesis/available/etd-06212013-172343/ © 2013 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria application/pdf University of Pretoria |
| spellingShingle | UCTD The effect of florfenicol on egg hatchability in fowls |
| title | The effect of florfenicol on egg hatchability in fowls |
| title_full | The effect of florfenicol on egg hatchability in fowls |
| title_fullStr | The effect of florfenicol on egg hatchability in fowls |
| title_full_unstemmed | The effect of florfenicol on egg hatchability in fowls |
| title_short | The effect of florfenicol on egg hatchability in fowls |
| title_sort | effect of florfenicol on egg hatchability in fowls |
| topic | UCTD |
| url | http://hdl.handle.net/2263/30951 http://upetd.up.ac.za/thesis/available/etd-06212013-172343/ |