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Genetic linkage map and QTL analysis of a cross between Fusarium subglutinans and Fusarium circinatum
Thesis (PhD)--University of Pretoria, 2012.
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University of Pretoria
2013
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| _version_ | 1867613475525623808 |
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| access_status_str | Open Access |
| author2 | Wingfield, Brenda D. |
| author_browse | Wingfield, Brenda D. |
| author_facet | Wingfield, Brenda D. |
| collection | Thesis |
| dc_rights_str_mv | © 2012 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Thesis (PhD)--University of Pretoria, 2012. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/31253 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:36:44.480Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/31253 Genetic linkage map and QTL analysis of a cross between Fusarium subglutinans and Fusarium circinatum Wingfield, Brenda D. Wingfield, Michael J. Myburg, Alexander Andrew De Vos, Lieschen UCTD Thesis (PhD)--University of Pretoria, 2012. The Gibberella fujikuroi species complex accommodates Fusarium spp. in the section Liseola that are unified by a common Gibberella sexual state. These are ubiquitous and economically important pathogens of plants, resulting in crop losses and the production of health-threatening mycotoxins. In this thesis, an interspecific cross between Fusarium circinatum and Fusarium subglutinans, both members of the G. fujikuroi species complex, were examined. A genetic linkage map was produced using Amplified Fragment Length Polymorphisms (AFLPs) and twelve linkage groups were identified. Distortion of the expected segregation patterns of these markers was observed in half of the AFLP markers genotyped. Distorted markers were found distributed throughout the genetic linkage map, leading to the hypothesis that distortion was caused by the high level of genomic divergence between the parental isolates. This was evident in that distorted alleles displayed genomewide skewing towards the F. subglutinans parental genotype, as well as the differential transmission of F. subglutinans alleles to F1 individuals. Investigations of Quantitative Trait Loci (QTL) involved in mycelial growth of the F1 progeny at 25 and 30˚C, suggested that a principle genomic region was involved. Localization of this QTL to the genomic sequence of F. circinatum revealed a putative fungal transcription factor. The presence of this transcription factor was detected only in F. circinatum, and not in F. verticillioides or F. fujikuroi, the only additional species in the G. fujikuroi species complex for which genomic data are available. The AFLP map allowed investigation of synteny among chromosomes from F. circinatum, F. verticillioides and F. fujikuroi. A significant level of macrosynteny was observed between representatives of the African, American and Asian clades of the G. fujikuroi species complex, respectively. Evidence was also found for a putative reciprocal translocation present only in F. circinatum and F. subglutinans. This region was found to be translocated relative to the genomic sequence of both F. verticillioides and F. fujikuroi. Jointly, these results provided considerable insight, at the genomic level, for species within the G. fujikuroi species complex. Importantly, the utility of a genetic map together with genomic sequence data was demonstrated, and the genetic basis of a growth QTL determined. Genetics Unrestricted 2013-09-09T12:09:28Z 2012-09-14 2013-09-09T12:09:28Z 2012-09-07 2012-09-14 2012-09-06 Thesis De Vos, L 2012, Genetic linkage map and QTL analysis of a cross between Fusarium subglutinans and Fusarium circinatum, PhD thesis, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/31253 > D12/9/48/ag http://hdl.handle.net/2263/31253 http://upetd.up.ac.za/thesis/available/etd-09062012-152611/ © 2012 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Genetic linkage map and QTL analysis of a cross between Fusarium subglutinans and Fusarium circinatum |
| title | Genetic linkage map and QTL analysis of a cross between Fusarium subglutinans and Fusarium circinatum |
| title_full | Genetic linkage map and QTL analysis of a cross between Fusarium subglutinans and Fusarium circinatum |
| title_fullStr | Genetic linkage map and QTL analysis of a cross between Fusarium subglutinans and Fusarium circinatum |
| title_full_unstemmed | Genetic linkage map and QTL analysis of a cross between Fusarium subglutinans and Fusarium circinatum |
| title_short | Genetic linkage map and QTL analysis of a cross between Fusarium subglutinans and Fusarium circinatum |
| title_sort | genetic linkage map and qtl analysis of a cross between fusarium subglutinans and fusarium circinatum |
| topic | UCTD |
| url | http://hdl.handle.net/2263/31253 http://upetd.up.ac.za/thesis/available/etd-09062012-152611/ |