Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Evaluation of two viral vectors for virus-induced gene silencing in pearl millet
Dissertation (MSc)--University of Pretoria, 2011.
Saved in:
| Other Authors: | |
|---|---|
| Format: | Thesis |
| Published: |
University of Pretoria
2013
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613709941080064 |
|---|---|
| access_status_str | Open Access |
| author2 | Berger, David Kenneth |
| author_browse | Berger, David Kenneth |
| author_facet | Berger, David Kenneth |
| collection | Thesis |
| dc_rights_str_mv | © 2011, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2011. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/31294 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:40:28.124Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/31294 Evaluation of two viral vectors for virus-induced gene silencing in pearl millet Berger, David Kenneth irene.schoeman@up.ac.za Hein, I. Schoeman, Irene UCTD Dissertation (MSc)--University of Pretoria, 2011. Pearl millet [Pennisetum glaucum L. Br.] is an agriculturally important cereal farmed in the marginal semi-arid regions of sub-Saharan Africa and India. The crop shows remarkable tolerance to both biotic and abiotic stress and several genetic components involved in these traits have already been identified. To date little progress has been made towards the development of a molecular technique to functionally characterise such candidate genes in pearl millet. One such potential method is virus-induced gene silencing (VIGS), a nucleotide sequence homology dependent technique used to down-regulate expression of selected genes using a recombinant viral vector. VIGS has been applied with great success in dicotyledonous and more recently monocotyledonous plant species. The objective of this study was to evaluate two viruses, barley stripe mosaic virus (BSMV) and brome mosaic virus (BMV), as VIGS vectors for pearl millet. Our hypothesis was that these viruses, which have successfully been used to conduct VIGS in other monocotyledonous species, could potentially be transferred to perform gene silencing in pearl millet. The viral vectors were modified to target a phenotypic marker gene, Phytoene desaturase (Pds), in pearl millet. Triticum aestivum (wheat), a known host for BSMV and Hordeum vulgare (barley), a known host for BMV and BSMV, were used as positive controls of viral infection. No visual Pds silencing symptoms were observed in the two pearl millet breeding lines, ICML12 and ICMB96222. Phenotypic symptoms associated with Pds silencing were observed in wheat seedlings infected with the Pds silencing BSMV construct. No significant distinction could be made between viral symptoms and potential Pds silencing in barley indicating that the pearl millet Pds insert in the BMV construct was insufficient to induce visual Pds silencing in barley. The presence of the viruses in systemic pearl millet leaves was evaluated using reverse transcription PCR targeted to all three genomic subunits of the respective viruses. Virus-specific products could be amplified from the barley and wheat accessions used as positive controls but it was found that neither of the viruses was present in pearl millet. This indicated that the VIGS vectors; BSMV and BMV were not amenable to conduct gene silencing in pearl millet under the conditions tested in this study. Although this study did not identify a VIGS vector, it does present some advances made towards the identification of a gene silencing system in pearl millet. Plant Science Unrestricted 2013-09-09T12:10:25Z 2011-09-23 2013-09-09T12:10:25Z 2011-09-09 2011-09-23 2011-09-23 Dissertation Schoeman, I 2011, Evaluation of two viral vectors for virus-induced gene silencing in pearl millet, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/31294 > E11/9/167/hv http://hdl.handle.net/2263/31294 http://upetd.up.ac.za/thesis/available/etd-09232011-134556/ © 2011, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Evaluation of two viral vectors for virus-induced gene silencing in pearl millet |
| title | Evaluation of two viral vectors for virus-induced gene silencing in pearl millet |
| title_full | Evaluation of two viral vectors for virus-induced gene silencing in pearl millet |
| title_fullStr | Evaluation of two viral vectors for virus-induced gene silencing in pearl millet |
| title_full_unstemmed | Evaluation of two viral vectors for virus-induced gene silencing in pearl millet |
| title_short | Evaluation of two viral vectors for virus-induced gene silencing in pearl millet |
| title_sort | evaluation of two viral vectors for virus induced gene silencing in pearl millet |
| topic | UCTD |
| url | http://hdl.handle.net/2263/31294 http://upetd.up.ac.za/thesis/available/etd-09232011-134556/ |