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The effects of clofazimine on mycobacterium smegmatis biofilm formation
Dissertation (MSc)--University of Pretoria, 2013.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2013
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| _version_ | 1867613568213450752 |
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| access_status_str | Open Access |
| author2 | Cholo, Moloko C. |
| author_browse | Cholo, Moloko C. |
| author_facet | Cholo, Moloko C. |
| collection | Thesis |
| dc_rights_str_mv | © 2000, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2013. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/31569 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:38:12.930Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/31569 The effects of clofazimine on mycobacterium smegmatis biofilm formation Cholo, Moloko C. t.mothiba@yahoo.com Mothiba, Maborwa Tebogo Free mycolic acid Biofilm Planktonic Antimycobacterial activity Nanoparticle Clofazimine Chemotherapy Granuloma Mycobacterium smegmatis Mycobacterium tuberculosis Dissertation (MSc)--University of Pretoria, 2013. Chemotherapy of tuberculosis (TB), a disease caused by Mycobacterium tuberculosis (M. tuberculosis), is successful against actively-growing bacilli but ineffective against dormant/persistent organisms, found mainly in a protective lipid-laden granuloma, possibly necessitating the use of lipophilic antibiotics. In vitro, these bacilli are encased in lipid-rich biofilms. In this study, the antimycobacterial activity of one such agent, clofazimine, and its nanoparticle formulation, have been investigated against Mycobacterium smegmatis (M. smegmatis), as a surrogate for M. tuberculosis, by determining the bacteriostatic and bactericidal activities of the native (NC) and spray-dried (SDC) preparations of this agent on planktonic and biofilm populations, as well as their effects on biofilm formation and its lipid compositions, specifically free mycolic acid (FM) content. Both preparations were comparable, being bacteriostatic for rapidly-proliferating bacilli, bactericidal for slow-growing, biofilm-producing sessile bacteria, but ineffective against non-replicating, biofilm-encased M. smegmatis organisms. However, similar studies in M. tuberculosis are required. Immunology Unrestricted 2013-09-10T07:01:00Z 2013 2013-09-10T07:01:00Z 2013 2013 2013-07-05 Dissertation Mothiba, MT 2013, The effects of clofazimine on mycobacterium smegmatis biofilm formation, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd<http://hdl.handle.net/2263/31569 > C13/9/948/gm http://hdl.handle.net/2263/31569 en © 2000, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Free mycolic acid Biofilm Planktonic Antimycobacterial activity Nanoparticle Clofazimine Chemotherapy Granuloma Mycobacterium smegmatis Mycobacterium tuberculosis The effects of clofazimine on mycobacterium smegmatis biofilm formation |
| title | The effects of clofazimine on mycobacterium smegmatis biofilm formation |
| title_full | The effects of clofazimine on mycobacterium smegmatis biofilm formation |
| title_fullStr | The effects of clofazimine on mycobacterium smegmatis biofilm formation |
| title_full_unstemmed | The effects of clofazimine on mycobacterium smegmatis biofilm formation |
| title_short | The effects of clofazimine on mycobacterium smegmatis biofilm formation |
| title_sort | effects of clofazimine on mycobacterium smegmatis biofilm formation |
| topic | Free mycolic acid Biofilm Planktonic Antimycobacterial activity Nanoparticle Clofazimine Chemotherapy Granuloma Mycobacterium smegmatis Mycobacterium tuberculosis |
| url | http://hdl.handle.net/2263/31569 |