Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Ultrstructural and flow cytometric analysis of platelets and fibrin networks during the menstrual cycle and pregnancy
Thesis (PhD)--University of Pretoria, 2013.
Saved in:
| Other Authors: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
University of Pretoria
2014
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613685031108608 |
|---|---|
| access_status_str | Open Access |
| author2 | Pretorius, Elna |
| author_browse | Pretorius, Elna |
| author_facet | Pretorius, Elna |
| collection | Thesis |
| dc_rights_str_mv | © 2013 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Thesis (PhD)--University of Pretoria, 2013. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/32969 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:40:03.959Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/32969 Ultrstructural and flow cytometric analysis of platelets and fibrin networks during the menstrual cycle and pregnancy Pretorius, Elna Lindeque, B. Gerhard Swanepoel, A.C. (Albe Carina) Women Pregnancy Menstrual cycle Hormones Blood coagulation UCTD Thesis (PhD)--University of Pretoria, 2013. INTRODUCTION: The menstrual cycle and pregnancy are processes unique to women. Both these processes involve various hormones as well as the coagulation system. Throughout normal pregnancy, platelet activation and increase in blood coagulation factors contributes to the hypercoagulable state observed on a physiological level. METHODS: Fibrin networks and platelets were analysed by electron microscopy and flow cytometry to determine any differences found in different phases of pregnancy compared to healthy control individuals. The fibrin networks from different phases of the menstrual cycle as well as different phases of pregnancy were investigated. RESULTS: It was found that ultrastructural changes in fibrin fiber morphology result from estrogen changes during the menstrual cycle. During pregnancy the minor thin fibers were prominent and thick matted layers of coagulant formation were evident. A large quantity of protein globular clusters similar to those seen in the menstrual cycle was present. Changes observed in platelet ultrastructure during pregnancy showed pregnancy-specific modifications. Platelets were activated and internal organelles showed variation from control participants. Flow cytometric analysis of platelets verified pregnancy-specific modifications. Close interactions between platelets and erythrocytes were evident. CONCLUSION: The female body is equipped to handle alterations in the coagulation system as can be extrapolated from the pregnancy-specific modifications. This study is the first to show alterations in fibrin network and platelet ultrastructure during and after pregnancy when compared to non-pregnant controls. The physiological changes during normal pregnancy can be used as a standard for comparison to abnormal or ailing pregnancy. gm2013 Physiology Unrestricted 2014-01-13T12:29:19Z 2014-01-13T12:29:19Z 2013-09-06 2013 Thesis Swanepoel, AC 2013, 'Ultrstructural and flow cytometric analysis of platelets and fibrin networks during the menstrual cycle and pregnancy', PhD thesis, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/32969> D13/9/969/gm http://hdl.handle.net/2263/32969 en © 2013 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Women Pregnancy Menstrual cycle Hormones Blood coagulation UCTD Ultrstructural and flow cytometric analysis of platelets and fibrin networks during the menstrual cycle and pregnancy |
| title | Ultrstructural and flow cytometric analysis of platelets and fibrin networks during the menstrual cycle and pregnancy |
| title_full | Ultrstructural and flow cytometric analysis of platelets and fibrin networks during the menstrual cycle and pregnancy |
| title_fullStr | Ultrstructural and flow cytometric analysis of platelets and fibrin networks during the menstrual cycle and pregnancy |
| title_full_unstemmed | Ultrstructural and flow cytometric analysis of platelets and fibrin networks during the menstrual cycle and pregnancy |
| title_short | Ultrstructural and flow cytometric analysis of platelets and fibrin networks during the menstrual cycle and pregnancy |
| title_sort | ultrstructural and flow cytometric analysis of platelets and fibrin networks during the menstrual cycle and pregnancy |
| topic | Women Pregnancy Menstrual cycle Hormones Blood coagulation UCTD |
| url | http://hdl.handle.net/2263/32969 |