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Characterizing mutagenesis in Fusarium circinatum
Dissertation (MSc)--University of Pretoria, 2013.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2014
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| _version_ | 1867613588652294144 |
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| access_status_str | Open Access |
| author2 | Steenkamp, Emma Theodora |
| author_browse | Steenkamp, Emma Theodora |
| author_facet | Steenkamp, Emma Theodora |
| collection | Thesis |
| dc_rights_str_mv | © 2013 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2013. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/32988 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:38:32.434Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/32988 Characterizing mutagenesis in Fusarium circinatum Steenkamp, Emma Theodora Fourie, G. Wingfield, Brenda D. Van Coller, Sophia Johanna Spontaneous mutagenesis Species Fusarium circinatum Fusarium species UCTD Dissertation (MSc)--University of Pretoria, 2013. Spontaneous mutagenesis can be divided into three main steps: the introduction of DNA damage and lesions, damage recognition and DNA repair. All sources of spontaneous mutagenesis originate from within the cell itself, e.g., polymerase errors cause DNA mismatches and reactive oxygen species alter the chemical composition of DNA bases. The combined effects of all these processes influence spontaneous genomic mutation rates, which are thought to be a characteristic of individual species and/or groups of species. Although much is known about different mutagens and how they cause mutations the sequence context of these mutations are less well understood. The results of this MSc study on mutation in the filamentous fungus Fusarium circinatum showed that the 5ʹ and 3ʹ neighbouring bases of a single nucleotide polymorphism can significantly influence the type of substitution that occurred leading to the formation of mutational motifs. This was the case for both sets of genes examined (core housekeeping and non-ribosomal protein synthetase genes), whose evolution is known to differ. The fact that none of the identified motifs are shared between the two sets of genes could indicate that the cellular mutagens and/or repair machinery function differently for the two gene groups. Furthermore, none of the mutable motifs that have been identified for the well-known mutagens in model organisms could be detected in the fungus, which suggests that mutagens and/or DNA repair mechanisms of this fungus are unique. Although limited information is available for non-model eukaryotes, an estimate for the rate at which mutations arise across the genome of F. circinatum could be a good starting point for comparisons of its evolutionary rate to those of its close relatives. This was accomplished using a fluctuation analysis involving nitrate non-utilizing mutation reversion. Although mutation rate determined in this study is probably not precisely accurate, it represents a good starting point for future comparative studies on the evolutionary rate of Fusarium species. As a whole this study laid the foundation for a better understanding of spontaneous mutagenesis at specific sites in certain groups of genes as well as across the genome of the economically important plant pathogen F. circinatum. Restricted until August 2017 gm2013 Microbiology and Plant Pathology Unrestricted 2014-01-15T11:52:55Z 2014-01-15T11:52:55Z 2013-09-06 2013 Dissertation Van Coller, SJ 2013, 'Characterizing mutagenesis in Fusarium circinatum', MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/32988> E13/9/864/gm http://hdl.handle.net/2263/32988 en © 2013 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Spontaneous mutagenesis Species Fusarium circinatum Fusarium species UCTD Characterizing mutagenesis in Fusarium circinatum |
| title | Characterizing mutagenesis in Fusarium circinatum |
| title_full | Characterizing mutagenesis in Fusarium circinatum |
| title_fullStr | Characterizing mutagenesis in Fusarium circinatum |
| title_full_unstemmed | Characterizing mutagenesis in Fusarium circinatum |
| title_short | Characterizing mutagenesis in Fusarium circinatum |
| title_sort | characterizing mutagenesis in fusarium circinatum |
| topic | Spontaneous mutagenesis Species Fusarium circinatum Fusarium species UCTD |
| url | http://hdl.handle.net/2263/32988 |