Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Establishment of tissue culture plants for the evaluation of defence-related genes in Persea americana Mill
Dissertation (MSc (Genetics))--University of Pretoria, 2013.
Saved in:
| Other Authors: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
University of Pretoria
2014
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613514001022976 |
|---|---|
| access_status_str | Open Access |
| author2 | Van den Berg, Noelani |
| author_browse | Van den Berg, Noelani |
| author_facet | Van den Berg, Noelani |
| collection | Thesis |
| dc_rights_str_mv | © 2013 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria |
| description | Dissertation (MSc (Genetics))--University of Pretoria, 2013. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/32991 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:37:20.986Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/32991 Establishment of tissue culture plants for the evaluation of defence-related genes in Persea americana Mill Van den Berg, Noelani Van Niekerk, Lindsay Avocado (Persea americana) Vitamin C, K and B6 Persea americana Mill Fruit Phytophthora cinnamomi Rossellinia necatrix UCTD Dissertation (MSc (Genetics))--University of Pretoria, 2013. The avocado (Persea americana Mill.) is a popular fruit crop eaten throughout the world. It has a number of health benefits and uses and is a good source of fibre, potassium, Vitamin C, K and B6, folate, and antioxidants. Numerous breeding programs for avocado exist worldwide and some of the characteristics which are selected for include tolerance to white root rot caused by Rossellinia necatrix, rootstocks with increased salt tolerance and scion breeding objectives which include the improvement of fruit quality and tolerance to post-harvest diseases. One of the most important breeding objectives however, is the selection of rootstocks which show increased tolerance to Phytophthora cinnamomi Rands. The development of new cultivars is very slow, since the avocado has a high level of heterozygosity which makes it difficult to predict the genotype of the progeny. Furthermore, the avocado also has a very long juvenile phase and it takes extended periods of time for new cultivars to be evaluated in the field. Also, very little is known about the molecular mechanisms involved in plant-pathogen interactions. Plant tissue culture techniques may be a useful tool to help accelerate plant breeding strategies. The aim of this MSc was therefore to evaluate different tissue culture techniques and media in order to produce plant material which can be used to aid in the development of new cultivars as well as molecular studies. Defence-related genes in avocado in response to P. cinnamomi were also investigated in five different rootstocks to gain a better understanding of the plant-pathogen interaction mechanism and the tolerance/susceptible response in avocado. Chapter 1 provides a comprehensive overview of the developmental and reproductive biology of the avocado as well as its interaction with the oomycete, P. cinnamomi. In addition, plant tissue culture and the progress made in the in vitro culture and transformation of avocado is also discussed. Chapter 2 describes the evaluation of different tissue culture techniques in order to find the most efficient and reliable method for the germination, micropropagation, rooting, acclimatization and callus initiation of avocado zygotic embryos. The use of in vitro generated plant material in pathogenicity trials and for gene expression studies is also evaluated. In Chapter 3, the expression of four defence-related genes is studied in five rootstock cultivars over seven different time points after inoculation with P. cinnamomi. Chapter 4 provides a general discussion in which the results obtained and conclusions drawn from the two experimental chapters and the Literature review are discussed along with some of the future applications of the results. gm2013 Genetics Unrestricted 2014-01-15T11:53:12Z 2014-01-15T11:53:12Z 2013-09-06 2013 Dissertation Van Niekerk, L 2013, 'Establishment of tissue culture plants for the evaluation of defence-related genes in Persea americana Mill', MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/32991> E13/9/864/gm http://hdl.handle.net/2263/32991 en © 2013 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria application/pdf University of Pretoria |
| spellingShingle | Avocado (Persea americana) Vitamin C, K and B6 Persea americana Mill Fruit Phytophthora cinnamomi Rossellinia necatrix UCTD Establishment of tissue culture plants for the evaluation of defence-related genes in Persea americana Mill |
| title | Establishment of tissue culture plants for the evaluation of defence-related genes in Persea americana Mill |
| title_full | Establishment of tissue culture plants for the evaluation of defence-related genes in Persea americana Mill |
| title_fullStr | Establishment of tissue culture plants for the evaluation of defence-related genes in Persea americana Mill |
| title_full_unstemmed | Establishment of tissue culture plants for the evaluation of defence-related genes in Persea americana Mill |
| title_short | Establishment of tissue culture plants for the evaluation of defence-related genes in Persea americana Mill |
| title_sort | establishment of tissue culture plants for the evaluation of defence related genes in persea americana mill |
| topic | Avocado (Persea americana) Vitamin C, K and B6 Persea americana Mill Fruit Phytophthora cinnamomi Rossellinia necatrix UCTD |
| url | http://hdl.handle.net/2263/32991 |