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Molecular characterisation of the ornithine decarboxylase gene of the human malaria parasite, plasmidium falciparum
Dissertation (MSc)--University of Pretoria, 1998.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2014
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| _version_ | 1867613617761812480 |
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| access_status_str | Open Access |
| author2 | Louw, Abraham Izak |
| author_browse | Louw, Abraham Izak |
| author_facet | Louw, Abraham Izak |
| collection | Thesis |
| dc_rights_str_mv | © 1998 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 1998. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/37299 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:39:00.149Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/37299 Molecular characterisation of the ornithine decarboxylase gene of the human malaria parasite, plasmidium falciparum Louw, Abraham Izak Visser, L. Birkholtz, Lyn-Marie Malaria Tropical infectious diseases Molecular characterisation of the eDNA Human malaria parasite P. falciparum Ornithine decarboxylase (ODC) UCTD Dissertation (MSc)--University of Pretoria, 1998. Malaria is one of the most serious tropical infectious diseases affecting mankind. The prevention of the disease is hampered by the increasing resistance of the parasite to existing chemotherapy and -prophylaxis drugs. The need for novel therapeutic targets and drugs is therefore enormous and the understanding of the biochemistry of the parasite is imperative. The aim of this study was the identification and molecular characterisation of the eDNA of one such metabolic target protein, ornithine decarboxylase (ODC), in the human malaria parasite P. falciparum. The P. falciparum ODC eDNA was isolated by means of a modified RT-PCR technique, RACE. No sequence data were available and the primers used were based on consensus areas identified in the protein sequences from other related organisms. The isolation and identification of the eDNA with degenerate primers was successful in 3' -RACE, but necessitated the optimisation of the eDNA synthesis protocol and the use of total RNA as starting material. The sequence obtained facilitated the application of 5' -RACE with ODC-specific primers based on the 3' -RACE sequence data. The full-length ODC eDNA sequence was obtained by overlap-alignment of various segments. A novel suppression PCR technology was applied during the 5' -RACE in order to create an uncloned eDNA library of amplified cDNAs representing only the mRNA population. The P. falciparum ODC eDNA contains an open reading frame of ---2847 bp and translates to a large 939 amino acid protein. The protein contained large internal insertions and was extended by '""273 N-terminal residues compared to ODCs from other organisms. Several possible signature motifs were identified for phosphorylation, glycosylation and transamidation. The P. falciparum ODC protein seems to contain more hydrophilic and a-helix forming residues. These characteristics should be further investigated after expression of the recombinant protein. The isolation of the P. falciparum ODC eDNA facilitates the validation of this protein as an antimalarial target. gm2014 Biochemistry unrestricted 2014-04-01T09:11:08Z 2014-04-01T09:11:08Z 1998-12-02 1998 Dissertation Birkholtz, LM 1998, Molecular characterisation of the ornithine decarboxylase gene of the human malaria parasite, plasmidium falciparum, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/37299> E14/4/25/gm http://hdl.handle.net/2263/37299 en © 1998 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Malaria Tropical infectious diseases Molecular characterisation of the eDNA Human malaria parasite P. falciparum Ornithine decarboxylase (ODC) UCTD Molecular characterisation of the ornithine decarboxylase gene of the human malaria parasite, plasmidium falciparum |
| title | Molecular characterisation of the ornithine decarboxylase gene of the human malaria parasite, plasmidium falciparum |
| title_full | Molecular characterisation of the ornithine decarboxylase gene of the human malaria parasite, plasmidium falciparum |
| title_fullStr | Molecular characterisation of the ornithine decarboxylase gene of the human malaria parasite, plasmidium falciparum |
| title_full_unstemmed | Molecular characterisation of the ornithine decarboxylase gene of the human malaria parasite, plasmidium falciparum |
| title_short | Molecular characterisation of the ornithine decarboxylase gene of the human malaria parasite, plasmidium falciparum |
| title_sort | molecular characterisation of the ornithine decarboxylase gene of the human malaria parasite plasmidium falciparum |
| topic | Malaria Tropical infectious diseases Molecular characterisation of the eDNA Human malaria parasite P. falciparum Ornithine decarboxylase (ODC) UCTD |
| url | http://hdl.handle.net/2263/37299 |