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Detection of xanthomonas campestris PV. Mangiferaeindicae in mango plants
Dissertation (MSc Agric)--University of Pretoria, 1993.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2014
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| _version_ | 1867613504936083456 |
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| access_status_str | Open Access |
| author2 | Kotze, J.M. |
| author_browse | Kotze, J.M. |
| author_facet | Kotze, J.M. |
| collection | Thesis |
| dc_rights_str_mv | © 1993 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc Agric)--University of Pretoria, 1993. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/40197 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:37:12.475Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/40197 Detection of xanthomonas campestris PV. Mangiferaeindicae in mango plants Kotze, J.M. Verschoor, Jan Adrianus Sanders, Gina Mercia Mangiferaeindicae in mango plants Xanthomonas campestris UCTD Dissertation (MSc Agric)--University of Pretoria, 1993. The yearly losses incurred by bacterial blackspot disease are high. Often trees are asymptomatic, with the pathogen either in the resident phase or latent stage of infection. Detection of the pathogen in these asymptomatic trees is one of the most important means of controlling the disease. Isolates which consistently differed in virulence were isolated from symptomatic mango plants. These isolates could be categorised into four groups based upon differences in virulence. Monoclonal antibodies (mAbs) were successfully raised using separate and pooled isolates for immunisation. MAbsraised were of the lgG class and reacted with a proteinaceous epitope. These monoclonal antibodies could distinguish between different virulence groups of Xanthomonas campestris pv. mangiferaeindicae by means of Western Blot analysis. These antibodies were used along with a selective medium, BVGA for detection of epiphytic populations as well as latent infections in mango. An enrichment step prior to the enzyme- linked immunosorbent assay (ELISA) is important, since bacterial counts on trees with latent infections are too low to result in a positive signal. These techniques in combination are thus useful for detection and monitoring of the pathogen, which may play an important role in controlling the spread of the disease. gm2013 Microbiology and Plant Pathology unrestricted 2014-06-17T12:59:50Z 2014-06-17T12:59:50Z 1993-05-30 2013 Dissertation Sanders, GM 1993, Detection of xanthomonas campestris PV. Mangiferaeindicae in mango plants, MSc (Agric) dissertation, University of Pretoria, Pretoria, viewed yymmdd<http://hdl.handle.net/2263/40197> http://hdl.handle.net/2263/40197 en © 1993 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Mangiferaeindicae in mango plants Xanthomonas campestris UCTD Detection of xanthomonas campestris PV. Mangiferaeindicae in mango plants |
| title | Detection of xanthomonas campestris PV. Mangiferaeindicae in mango plants |
| title_full | Detection of xanthomonas campestris PV. Mangiferaeindicae in mango plants |
| title_fullStr | Detection of xanthomonas campestris PV. Mangiferaeindicae in mango plants |
| title_full_unstemmed | Detection of xanthomonas campestris PV. Mangiferaeindicae in mango plants |
| title_short | Detection of xanthomonas campestris PV. Mangiferaeindicae in mango plants |
| title_sort | detection of xanthomonas campestris pv mangiferaeindicae in mango plants |
| topic | Mangiferaeindicae in mango plants Xanthomonas campestris UCTD |
| url | http://hdl.handle.net/2263/40197 |