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In vitro and in vivo production of artemisinin by artemisia species
Dissertation (MSc)--University of Pretoria, 2013.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2014
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| _version_ | 1867613678720778241 |
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| access_status_str | Open Access |
| author2 | Meyer, J.J.M. (Jacobus Johannes Marion) |
| author_browse | Meyer, J.J.M. (Jacobus Johannes Marion) |
| author_facet | Meyer, J.J.M. (Jacobus Johannes Marion) |
| collection | Thesis |
| dc_rights_str_mv | © 2013 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2013. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/40342 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:39:58.102Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/40342 In vitro and in vivo production of artemisinin by artemisia species Meyer, J.J.M. (Jacobus Johannes Marion) fjlkruger@gmail.com Kruger, Francois Jacobus Liebenberg Antimalarial treatments Leaves of Artemisia annua UCTD Dissertation (MSc)--University of Pretoria, 2013. Artemisinin is produced in the leaves of Artemisia annua and is currently one of the most valuable antimalarial treatments. A. annua is of Asian origin but many other family members have been identified worldwide. A. annua however, is the only one that produces artemisinin. Synthetic production of artemisinin is not yet feasible, not to mention very expensive and the product yields are relatively low. The aims of this study were threefold: 1) To regenerate callus, cell cultures and plants from genetically modified root cultures of A. afra into which an artemisinin biosynthetic gene was inserted from A. annua 2) To investigate the probability that fungal endophytes are responsible for the production of artemisinin and 3) To establish two fields of high yielding varieties of A. annua plants and evaluate whether artemisinin production of these two locations will remain high. Callus and cell cultures of the genetically modified A. afra root cultures were established, but no shoots have been produced as of yet and this is an on-going investigation. Fungal endophytes were sampled and none of the endophytes produced artemisinin. Five different lines of A. annua were cultivated, successfully grown and harvested. Measurements were taken at different stages of processing, these were compared and analysed using various methods such as height and mass comparisons. Comparisons revealed that the production of artemisinin is correlated to local sets of conditions rather than the variety of individual lines. The genetic potential to produce high quantities of artemisinin appears to have been lost, instead of being maintained. We confirmed that secondary compound production and specifically, artemisinin, is enhanced by certain stress factors on the plants. gm2014 Plant Science unrestricted 2014-06-24T09:48:26Z 2014-06-24T09:48:26Z 2014-04-09 2013 Dissertation Kruger, FJL 2013, In vitro and in vivo production of artemisinin by artemisia species, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/40342> E14/4/169/gm http://hdl.handle.net/2263/40342 en © 2013 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Antimalarial treatments Leaves of Artemisia annua UCTD In vitro and in vivo production of artemisinin by artemisia species |
| title | In vitro and in vivo production of artemisinin by artemisia species |
| title_full | In vitro and in vivo production of artemisinin by artemisia species |
| title_fullStr | In vitro and in vivo production of artemisinin by artemisia species |
| title_full_unstemmed | In vitro and in vivo production of artemisinin by artemisia species |
| title_short | In vitro and in vivo production of artemisinin by artemisia species |
| title_sort | in vitro and in vivo production of artemisinin by artemisia species |
| topic | Antimalarial treatments Leaves of Artemisia annua UCTD |
| url | http://hdl.handle.net/2263/40342 |