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Characterization of tolerance to bacterial wilt in the model plant Arabidopsis
Dissertation (MSc)--University of Pretoria, 2014.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2014
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| _version_ | 1867613630013374464 |
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| access_status_str | Open Access |
| author2 | Berger, David Kenneth |
| author_browse | Berger, David Kenneth |
| author_facet | Berger, David Kenneth |
| collection | Thesis |
| dc_rights_str_mv | © 2014 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2014. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/40348 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:39:11.828Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/40348 Characterization of tolerance to bacterial wilt in the model plant Arabidopsis Berger, David Kenneth bredenkamp.jane@gmail.com Naidoo, Sanushka Bredenkamp, Jane Ralstonia solanacearum Bacterial wilt disease Molecular plant pathologists African Eucalyptus UCTD Dissertation (MSc)--University of Pretoria, 2014. Ralstonia solanacearum, the causal agent of bacterial wilt disease, has been found to affect numerous economically important plants. Understanding the molecular basis of resistance, tolerance and susceptibility of plants to pathogens such as R. solanacearum is a major goal of molecular plant pathologists. Prior to this study it was thought that Arabidopsis accession Kil-0 shows gene-for-gene “resistance” to an African Eucalyptus isolate of R. solanacearum, BCCF402. However, a subsequent preliminary study indicated that Kil-0 may exhibit “tolerance” which is defined as the plant’s ability to support high pathogen numbers without displaying disease symptoms or a reduction in host fitness. The aim of this study was to determine if Kil-0 was tolerant to R. solanacearum BCCF402. The bacterial load of R. solanacearum was quantified in accessions Kil-0 and Be-0 using dilution plating and quantitative PCR methods. The cytC gene region was used to quantify R. solanacearum in Arabidopsis plants and the amount of bacterial DNA was normalized to “alien” DNA that was spiked into each sample. High bacterial concentrations of BCCF402 were found in Kil-0 but plants exhibited no wilting symptoms. Additionally, Kil-0 plants inoculated with BCCF402 showed no significant reduction in fitness compared to control Kil-0 plants. In contrast, high bacterial numbers and severe disease symptoms were observed in the susceptible Be-0 plants, whereas Nd1 plants contained a low number of bacteria and no disease symptoms indicative of a resistance response. These results illustrated that Kil-0 is tolerant to R. solanacearum isolate BCCF402. A tool for the visualization of R. solanacearum in Arabidopsis plants was designed. R. solanacearum isolate BCCF402 was tagged with two mCherry-containing plasmids under the constitutive expression of the tac promoter. The expression levels of mCherry were suitable for successful visualization in planta. BCCF402 cells transformed with the mCherry-containing plasmids were not affected in terms of virulence or disease progression compared to wildtype BCCF402 cells. A plasmid loss of 30-35% was observed in mCherry-tagged BCCF402 cells at later stages of Arabidopsis infection. mCherry-tagged BCCF402 was successfully visualized in Kil-0 leaves at early infection stages. gm2014 Plant Science unrestricted 2014-06-24T09:49:30Z 2014-06-24T09:49:30Z 2014-04-09 2014 Dissertation Bredenkamp, J 2014, Characterization of tolerance to bacterial wilt in the model plant Arabidopsis, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/40348> E14/4/171/gm http://hdl.handle.net/2263/40348 en © 2014 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Ralstonia solanacearum Bacterial wilt disease Molecular plant pathologists African Eucalyptus UCTD Characterization of tolerance to bacterial wilt in the model plant Arabidopsis |
| title | Characterization of tolerance to bacterial wilt in the model plant Arabidopsis |
| title_full | Characterization of tolerance to bacterial wilt in the model plant Arabidopsis |
| title_fullStr | Characterization of tolerance to bacterial wilt in the model plant Arabidopsis |
| title_full_unstemmed | Characterization of tolerance to bacterial wilt in the model plant Arabidopsis |
| title_short | Characterization of tolerance to bacterial wilt in the model plant Arabidopsis |
| title_sort | characterization of tolerance to bacterial wilt in the model plant arabidopsis |
| topic | Ralstonia solanacearum Bacterial wilt disease Molecular plant pathologists African Eucalyptus UCTD |
| url | http://hdl.handle.net/2263/40348 |