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Production and characterization of transgenic Arabidopsis and soybean (Glycine max (L.) Merrill.) plants over-expressing oryzacystatin I (OC-I)
Thesis (PhD)--University of Pretoria, 2014.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2015
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| _version_ | 1867613635193339904 |
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| access_status_str | Open Access |
| author2 | Kunert, Karl J. |
| author_browse | Kunert, Karl J. |
| author_facet | Kunert, Karl J. |
| collection | Thesis |
| dc_rights_str_mv | © 2014 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Thesis (PhD)--University of Pretoria, 2014. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/43160 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:39:16.707Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/43160 Production and characterization of transgenic Arabidopsis and soybean (Glycine max (L.) Merrill.) plants over-expressing oryzacystatin I (OC-I) Kunert, Karl J. eugene.makgopa@fabi.up.ac.za Foyer, Christine H. Makgopa, M.E. (Matome Eugene) UCTD Thesis (PhD)--University of Pretoria, 2014. In legumes, drought causes early senescence due to loss of the symbiotic relationship between the plant and the rhizobacterium. Senescence is characterized by increases in proteolytic enzymes required for protein recycling to other plant tissues. Transgenic soybean plants over-expressing a cysteine protease inhibitor (OC-I) were successfully generated and characterized. Plants of transgenic lines had differential transgene expression. Transgenic plants had lower protease activity determined by both an in-gel assay and in a fluoremetric assay using SDS-PAGE and fluorogenic protease substrate, respectively. Transgenic Arabidopsis plants over-expressing OC-I, generated by floral dip method, were more drought-tolerant compared to non-transgenic Arabidopsis plants. Seeds of different transgenic soybean lines had a lower germination rate and these transgenic lines had fewer leaves and shorter stems. Under drought stress, plants of transgenic lines performed better than wild-type non-transgenic plants with CO2 assimilation (photosynthesis) and better instantaneous water-use efficiencies (IWUE). In particular, plants of one transgenic line (line 57) appeared to be more drought-tolerant when compared to plants of all other tested lines. These transgenic plants retained more soil water and also had fewer leaves when compared to wild-type non-transgenic plants. Results obtained in this study have provided evidence that preventing cysteine protease activity by over-expressing a protease inhibitor causes phenotypic changes of the plant demonstrating an important role of cysteine proteases in plant growth and development and plant stress. Future work will focus on identifying these OC-I sensitive proteases and investigating their individual function in plant growth and development and stress. lk2014 Plant Science PhD Unrestricted 2015-01-19T12:11:09Z 2015-01-19T12:11:09Z 2014/12/12 2014 Thesis Makgopa, ME 2014, Production and characterization of transgenic Arabidopsis and soybean (Glycine max (L.) Merrill.) plants over-expressing oryzacystatin I (OC-I), PhD Thesis, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/43160> D14/9/29 http://hdl.handle.net/2263/43160 en © 2014 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Production and characterization of transgenic Arabidopsis and soybean (Glycine max (L.) Merrill.) plants over-expressing oryzacystatin I (OC-I) |
| title | Production and characterization of transgenic Arabidopsis and soybean (Glycine max (L.) Merrill.) plants over-expressing oryzacystatin I (OC-I) |
| title_full | Production and characterization of transgenic Arabidopsis and soybean (Glycine max (L.) Merrill.) plants over-expressing oryzacystatin I (OC-I) |
| title_fullStr | Production and characterization of transgenic Arabidopsis and soybean (Glycine max (L.) Merrill.) plants over-expressing oryzacystatin I (OC-I) |
| title_full_unstemmed | Production and characterization of transgenic Arabidopsis and soybean (Glycine max (L.) Merrill.) plants over-expressing oryzacystatin I (OC-I) |
| title_short | Production and characterization of transgenic Arabidopsis and soybean (Glycine max (L.) Merrill.) plants over-expressing oryzacystatin I (OC-I) |
| title_sort | production and characterization of transgenic arabidopsis and soybean glycine max l merrill plants over expressing oryzacystatin i oc i |
| topic | UCTD |
| url | http://hdl.handle.net/2263/43160 |