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Evaluation of Random Amplified Polymorphic DNA and Simple Sequence Repeat markers in Moringa oleifera (Lam.) to establish population diversity
Dissertation (MSc)--University of Pretoria, 2013.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2015
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| _version_ | 1867613449151840256 |
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| access_status_str | Open Access |
| author2 | Vorster, Barend Juan |
| author_browse | Vorster, Barend Juan |
| author_facet | Vorster, Barend Juan |
| collection | Thesis |
| dc_rights_str_mv | © 2014 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2013. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/43245 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:36:19.431Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/43245 Evaluation of Random Amplified Polymorphic DNA and Simple Sequence Repeat markers in Moringa oleifera (Lam.) to establish population diversity Vorster, Barend Juan Rynhard.smit@fabi.up.ac.za Du Toit, Elsie Sophia Smit, Rynhard UCTD Dissertation (MSc)--University of Pretoria, 2013. Moringa oleifera is potentially an economically important tree species. It has gained interest globally for its multipurpose uses, in particular as a source of nutrition and oil, as well as for its various medicinal properties. Moringa is native to India, Malaysia and the Middle East, but have been introduced to many countries throughout Africa. There is however limited knowledge regarding the genetic variation of both native and introduced populations of Moringa, although phenotypic observations suggest the presence of significant genetic diversity. To do this we used Moringa collections from different locations including India, South Africa and Hawaii, with different cultivars present in the foreign samples. Molecular markers such as Random Amplified Polymorphic DNA (RAPD) and Simple Sequence Repeats (SSR) were evaluated for their efficiency as a marker system in Moringa, based on their success in other tropical tree population studies. The comparative analysis between the two revealed that both marker systems identified similar heterogeneity of 0.438 (RAPD) and 0.481 (SSR). However, the microsatellite marker was more effective for assessing genetic diversity, with a marker index (MI) value of 5.77 compared to 2.96 (RAPD), within our data set. Three major groups among the 71 accessions were clustered together in the dendogram and Principle Co-ordinate Analysis (PCoA), based on the genetic similarity matrices generated by both markers. Both cultivars were grouped together irrespective of origins, suggesting a relationship of genetic identity rather than geographical origins. The markers investigated in this study were found to be effective for determining genetic diversity, verifying higher genetic variation among the S.A. accessions of Moringas and distinguishing them from the cultivars investigated. This information could possibly be exploited for cultivar development in tree improvement programmes. lk2014 Genetics MSc Unrestricted 2015-01-19T12:13:16Z 2015-01-19T12:13:16Z 2014/12/12 2013 Dissertation Smit, R 2013, Evaluation of Random Amplified Polymorphic DNA and Simple Sequence Repeat markers in Moringa oleifera (Lam.) to establish population diversity, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/43245> M14/9/220 http://hdl.handle.net/2263/43245 en © 2014 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Evaluation of Random Amplified Polymorphic DNA and Simple Sequence Repeat markers in Moringa oleifera (Lam.) to establish population diversity |
| title | Evaluation of Random Amplified Polymorphic DNA and Simple Sequence Repeat markers in Moringa oleifera (Lam.) to establish population diversity |
| title_full | Evaluation of Random Amplified Polymorphic DNA and Simple Sequence Repeat markers in Moringa oleifera (Lam.) to establish population diversity |
| title_fullStr | Evaluation of Random Amplified Polymorphic DNA and Simple Sequence Repeat markers in Moringa oleifera (Lam.) to establish population diversity |
| title_full_unstemmed | Evaluation of Random Amplified Polymorphic DNA and Simple Sequence Repeat markers in Moringa oleifera (Lam.) to establish population diversity |
| title_short | Evaluation of Random Amplified Polymorphic DNA and Simple Sequence Repeat markers in Moringa oleifera (Lam.) to establish population diversity |
| title_sort | evaluation of random amplified polymorphic dna and simple sequence repeat markers in moringa oleifera lam to establish population diversity |
| topic | UCTD |
| url | http://hdl.handle.net/2263/43245 |