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An investigation of the effects of fulvic and humic acids on the absorption of selected drugs, vitamins and minerals using the everted mouse gut model
Dissertation (MSc)--University of Pretoria, 2015.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2015
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| _version_ | 1867613524648263680 |
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| access_status_str | Open Access |
| author2 | Cromarty, Allan Duncan |
| author_browse | Cromarty, Allan Duncan |
| author_facet | Cromarty, Allan Duncan |
| collection | Thesis |
| dc_rights_str_mv | © 2015 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2015. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/46044 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:37:31.320Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/46044 An investigation of the effects of fulvic and humic acids on the absorption of selected drugs, vitamins and minerals using the everted mouse gut model Cromarty, Allan Duncan Willis, Kirsten UCTD Humic substances Humic acid Carbohydrate-derived fulvic acid Everted mouse gut model Drug absorption Health sciences theses SDG-03 Health sciences theses SDG-17 Dissertation (MSc)--University of Pretoria, 2015. Humic substances, such as the closely related humic and fulvic acids are ubiquitous, naturally occurring organic macromolecules of complex but undefined structure. These compounds are known complexing agents due to their supramolecular like structures and are capable of binding a wide variety of compounds. Numerous studies have confirmed that humic and fulvic acids exhibit diverse medicinal and therapeutic properties. For this reason, alternative or “natural” medicinal preparations rich in these substances are being self-administered, often concomitantly with conventional drugs. The possibility exists that these humic substances, found in the alternative medicinal products, may result in drug-drug interactions and bind to simultaneously ingested drugs. Complex formation may affect absorption and alter overall bioavailability. Changes in these parameters may lead to reduced therapeutic effect or toxic side effects of prescribed drugs in patients. Similarly, these humic substances may bind to and alter the uptake of ingested nutrients, such as vitamins and minerals, obtained from food sources as well as dietary supplements. Changes in absorption may result in a loss of proper physiological functioning in the body or in unwanted effects of overdose. This study investigated the effect of fulvic and humic acids on the absorption of commonly administered classes of drugs, vitamins and minerals using the everted mouse gut model that was successfully used to assess the membrane transport of the test compounds. This model made use of everted segments of excised intestinal tissue placed in Krebs Ringer Buffer (pH7.4), where physiological functioning of the tissue is maintained for up to two hours after excision. The amount of test compound which crossed through the intestinal membrane without and in the presence of each humic substance was quantified using LC-MS/MS methods developed for each of the drugs and vitamins, and ICP-MS, in the case of the minerals. The amount of test compound absorbed alone was compared to the amount absorbed when in the presence of each humic substance. Changes in the uptake, for each test compound was noted, the extent of the absorption increase or decrease was compound specific. The changes in absorption observed could be attributed to changes in compound solubility and mechanism of transport across the intestinal membrane once in complex. Drugs and vitamins were seen to be more prone to decreases in absorption in the presence of the humic substances, whereas the majority of the minerals showed significantly increased absorption. Binding of the minerals to the humic substances through chelation, and not complex formation, could have a greater effect on compound solubility. Health care professionals, as well as individuals ingesting these and other substances concurrently, should be aware of the potential effects on absorption that may occur due to drug-drug interactions in order to avoid a loss of therapeutic/physiological activity or negative toxic symptoms. tm2015 em2026 Pharmacology MSc Unrestricted SDG-03: Good health and well-being SDG-17: Partnerships for the goals 2015-07-02T11:06:38Z 2015-07-02T11:06:38Z 2015/04/24 2015 Dissertation Willis, K 2015, An investigation of the effects of fulvic and humic acids on the absorption of selected drugs, vitamins and minerals using the everted mouse gut model, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/46044> A2015 http://hdl.handle.net/2263/46044 en © 2015 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Humic substances Humic acid Carbohydrate-derived fulvic acid Everted mouse gut model Drug absorption Health sciences theses SDG-03 Health sciences theses SDG-17 An investigation of the effects of fulvic and humic acids on the absorption of selected drugs, vitamins and minerals using the everted mouse gut model |
| title | An investigation of the effects of fulvic and humic acids on the absorption of selected drugs, vitamins and minerals using the everted mouse gut model |
| title_full | An investigation of the effects of fulvic and humic acids on the absorption of selected drugs, vitamins and minerals using the everted mouse gut model |
| title_fullStr | An investigation of the effects of fulvic and humic acids on the absorption of selected drugs, vitamins and minerals using the everted mouse gut model |
| title_full_unstemmed | An investigation of the effects of fulvic and humic acids on the absorption of selected drugs, vitamins and minerals using the everted mouse gut model |
| title_short | An investigation of the effects of fulvic and humic acids on the absorption of selected drugs, vitamins and minerals using the everted mouse gut model |
| title_sort | investigation of the effects of fulvic and humic acids on the absorption of selected drugs vitamins and minerals using the everted mouse gut model |
| topic | UCTD Humic substances Humic acid Carbohydrate-derived fulvic acid Everted mouse gut model Drug absorption Health sciences theses SDG-03 Health sciences theses SDG-17 |
| url | http://hdl.handle.net/2263/46044 |