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Sero‐prevalence of brucellosis in cattle in Swaziland

Dissertation (MSc)--University of Pretoria, 2015.

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Other Authors: Michel, Anita Luise
Format: Thesis
Language:English
Published: University of Pretoria 2015
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access_status_str Open Access
author2 Michel, Anita Luise
author_browse Michel, Anita Luise
author_facet Michel, Anita Luise
collection Thesis
dc_rights_str_mv © 2015 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.
description Dissertation (MSc)--University of Pretoria, 2015.
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institution University of Pretoria (South Africa)
language English
last_indexed 2026-06-10T12:37:11.117Z
license_str Other — see source repository
provenance_str_mv Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository
publishDate 2015
publishDateRange 2015
publishDateSort 2015
publisher University of Pretoria
publisherStr University of Pretoria
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source_str UPSpace — University of Pretoria Institutional Repository
spelling oai:repository.up.ac.za:2263/49382 Sero‐prevalence of brucellosis in cattle in Swaziland Michel, Anita Luise Ndwandwe, Bongekile K. UCTD Dissertation (MSc)--University of Pretoria, 2015. This study aimed at establishing the first cross‐sectional sero‐prevalence study of bovine brucellosis in the cattle population in Swaziland as well as technology transfer regarding the ELISA technique to allow an extended serological diagnosis of bovine brucellosis in the country. Twenty diptanks out of 778 functional diptanks were randomly selected for the cross‐sectional brucellosis survey across all the four regions of Swaziland. A total of 1623 bovine sera from individual cattle out of an estimated bovid population of 622 715 was tested for the presence of Brucella antibodies using the Rose Bengal test (RBT) and indirect Enzyme Linked Immunosorbent Assay (iELISA). The iELISA proved to be more sensitive than the RBT, overall. However, in some cases the RBT yielded more positive reactors than the iELISA and vice versa. The serological prevalence of bovine brucellosis at diptank level ranged from 0.0% to as high as 53.3% and 50.9%, as determined by the RBT and iELISA tests, respectively. Regional seroprevalence ranged from 3.05% to 53.05% by RBT and by iELISA, the sero‐prevalence ranged from 3.90% to 52.84% and in both tests Lubombo region had the highest sero‐prevalence and Hhohho region the least. Based on the findings of this study sero‐prevalence of bovine brucellosis across all sampled regions was 16.14% and 17.38% in RBT and iELISA test, respectively. However, to achieve maximum sensitivity in this survey a parallel interpretation of the test results achieved by the two tests was used and an overall sero‐prevalence of 21.50% was established. The western parts of the country exhibited a lower bovine brucellosis seroprevalence, however, a systematic national bovine brucellosis survey is recommended. tm2015 Veterinary Tropical Diseases MSc Unrestricted 2015-08-19T06:36:41Z 2015-08-19T06:36:41Z 2015 2015 Dissertation Ndwandwe, BK 2015, Sero ‐ prevalence of brucellosis in Cattle in Swaziland, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/49382> A2015 http://hdl.handle.net/2263/49382 en © 2015 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria
spellingShingle UCTD
Sero‐prevalence of brucellosis in cattle in Swaziland
title Sero‐prevalence of brucellosis in cattle in Swaziland
title_full Sero‐prevalence of brucellosis in cattle in Swaziland
title_fullStr Sero‐prevalence of brucellosis in cattle in Swaziland
title_full_unstemmed Sero‐prevalence of brucellosis in cattle in Swaziland
title_short Sero‐prevalence of brucellosis in cattle in Swaziland
title_sort sero prevalence of brucellosis in cattle in swaziland
topic UCTD
url http://hdl.handle.net/2263/49382