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The effects of diet varying in fat content on the pathogenesis of diabetes mellitus in wistar rat offspring
Dissertation (MSc)--University of Pretoria, 2015.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2016
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| _version_ | 1867613448379039744 |
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| access_status_str | Open Access |
| author2 | Grobbelaar, C.W. (Craig) |
| author_browse | Grobbelaar, C.W. (Craig) |
| author_facet | Grobbelaar, C.W. (Craig) |
| collection | Thesis |
| dc_rights_str_mv | © 2016 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2015. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/53032 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:36:18.633Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/53032 The effects of diet varying in fat content on the pathogenesis of diabetes mellitus in wistar rat offspring Grobbelaar, C.W. (Craig) nhlanhlasimphiwe@gmail.com Cerf, M.E. Mthembu, Nhlanhla Simphiwe Christa UCTD SDG-03: Good health and well-being SDG-17: Partnerships for the goals Dissertation (MSc)--University of Pretoria, 2015. Aims/hypothesis: The incidence of type 2 diabetes (T2D) is increasing globally. T2D is characterised by progressive deterioration of glycaemic control. These changes in glucose homeostasis are primarily due to ?-cell secretory dysfunction and/or peripheral insulin resistance. Studies show that maternal health directly influences foetal development and birth outcomes. Malnutrition of the growing foetus may lead to development of T2D and other metabolic diseases later in life. Consequently, several studies have reported that maternal diet programmes the foetus leading to altered physiology and metabolism in the offspring. Foetal programming refers to the exposure to a stimulus and/or insult during the critical periods of development i.e. foetal and early neonatal life. We therefore sought to ascertain how a dietary fat content in maternal diet affects foetal programming and its contribution to the pathogenesis of T2D of the offspring. Methods: Pregnant rats were randomly grouped and maintained on diets varying in fat content: 10% (Control), 20% (20F), 30% (30F) and 40% (40F) fat throughout their pregnancy. Pancreata were collected and quantitative polymerase chain reaction tests were performed to determine the mRNA expression profiles of the insulin signaling and transcription factors including Pdx1, MafB, IR?, insulin and glucagon. Other pancreata were immunostained followed by image analysis of these factors. Results: In 40F neonates, Ins1, Ins2, glucagon, MafB and IRS2 mRNA expression was reduced. Further, in 30F neonates, Ins1, Pdx1 and MafB mRNA expression was reduced. There were no changes in immunoreactivity for the factors studied. However, when separating the offspring according to gender, IR? immunoreactivity was reduced in 40F females compared to 40F males. Conclusion: Continuous exposure of pregnant rats to an excessively high fat diet impairs gene expression of key factors involved in insulin signaling and islet development in their neonatal offspring. This reflects foetal programming of metabolic pathways in insulin signaling and ?-cell development and function which potentially renders these offspring susceptible to metabolic disease and the development of T2D. em2026 Physiology MSc Unrestricted SDG-03: Good health and well-being SDG-17: Partnerships for the goals 2016-06-10T07:03:50Z 2016-06-10T07:03:50Z 2016-04-22 2015 Dissertation Mthembu, NSC 2015, The effects of diet varying in fat content on the pathogenesis of diabetes mellitus in wistar rat offspring, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/53032> A2016 http://hdl.handle.net/2263/53032 en © 2016 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD SDG-03: Good health and well-being SDG-17: Partnerships for the goals The effects of diet varying in fat content on the pathogenesis of diabetes mellitus in wistar rat offspring |
| title | The effects of diet varying in fat content on the pathogenesis of diabetes mellitus in wistar rat offspring |
| title_full | The effects of diet varying in fat content on the pathogenesis of diabetes mellitus in wistar rat offspring |
| title_fullStr | The effects of diet varying in fat content on the pathogenesis of diabetes mellitus in wistar rat offspring |
| title_full_unstemmed | The effects of diet varying in fat content on the pathogenesis of diabetes mellitus in wistar rat offspring |
| title_short | The effects of diet varying in fat content on the pathogenesis of diabetes mellitus in wistar rat offspring |
| title_sort | effects of diet varying in fat content on the pathogenesis of diabetes mellitus in wistar rat offspring |
| topic | UCTD SDG-03: Good health and well-being SDG-17: Partnerships for the goals |
| url | http://hdl.handle.net/2263/53032 |