Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Investigating the potentially expanded target repertoire of murinized Internalin of Listeria monocytogenes
Dissertation (MSc)--University of Pretoria, 2016.
Saved in:
| Other Authors: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
University of Pretoria
2016
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613701601755137 |
|---|---|
| access_status_str | Open Access |
| author2 | Schubert, Wolf-Dieter |
| author_browse | Schubert, Wolf-Dieter |
| author_facet | Schubert, Wolf-Dieter |
| collection | Thesis |
| dc_rights_str_mv | © 2016 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2016. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/56248 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:40:20.090Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/56248 Investigating the potentially expanded target repertoire of murinized Internalin of Listeria monocytogenes Schubert, Wolf-Dieter Motshwene, Precious G. Ndima, Daniel, Senzile. Listeria monocytogenes Murinized InlA Murine N-cadherin Human N-cadherin Protein-protein interactions UCTD Dissertation (MSc)--University of Pretoria, 2016. The ability of intracellular pathogens to invade and spread from non-phagocytic cell to another is an imperative mechanism broadly investigated in cellular biology. Listeria monocytogenes (Lm) –one example of intracellular pathogens, invades specifically human epithelial cells using its surface proteins Internalin A (InlA) and InlB, respectively. InlA alone is sufficient to internalise the pathogen into the host cells by interacting with human E-cadherin –specifically the N-terminal domain 1 (hEC1). The InlA variant (InlAm) that was previously made to increase the binding affinity to hEC1 was successfully engineered in this study. This variant was found to interact with N-terminal domain 1 of murine E-cadherin (mEC1) by isothermal titration calorimetry (ITC). Previously, the InlAm was reported to allow Lm invasion into M villous cells that express murine N-cadherin –possibly via the N-terminal domain 1 (mNC1). In this study, InlAm did not have affinity for mNC1 or N-terminal domain 1 of human N-cadherin (hNC1) when analysed by ITC –possibly due to amino acid sequences variation from both mEC1 and hEC1. However, by structurally engineering the complexes (InlAm/mNC1 and InlAm/hNC1) and studying their interaction interfaces, it was revealed that mNC1 and hNC1 can be recognised by InlAm just like hEC1. This was supported by the distances between interacting amino acid residues in InlAm/hEC1 crystal structure complex, which were also conserved in the engineered complexes. These observations related to the fact that the N-terminal domains of E- and N-cadherin are structurally conserved, therefore that could have attributed to similarities observed in the engineered complexes. Therefore, future studies would aim at using alternative methods that could support or disprove one of the two findings, that is whether InlAm and any of the N-terminal domains of N-cadherin interact or not. National Research Foundation (NRF) The Allan Gray Orbis Foundation The Mandela Rhodes Foundation Biochemistry MSc Unrestricted 2016-08-10T09:23:01Z 2016-08-10T09:23:01Z 2016-09-01 2016-07-25 Dissertation Ndima, D 2016, Investigating the potentially expanded target repertoire of murinized Internalin of Listeria monocytogenes, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/56248> http://hdl.handle.net/2263/56248 en © 2016 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Listeria monocytogenes Murinized InlA Murine N-cadherin Human N-cadherin Protein-protein interactions UCTD Investigating the potentially expanded target repertoire of murinized Internalin of Listeria monocytogenes |
| title | Investigating the potentially expanded target repertoire of murinized Internalin of Listeria monocytogenes |
| title_full | Investigating the potentially expanded target repertoire of murinized Internalin of Listeria monocytogenes |
| title_fullStr | Investigating the potentially expanded target repertoire of murinized Internalin of Listeria monocytogenes |
| title_full_unstemmed | Investigating the potentially expanded target repertoire of murinized Internalin of Listeria monocytogenes |
| title_short | Investigating the potentially expanded target repertoire of murinized Internalin of Listeria monocytogenes |
| title_sort | investigating the potentially expanded target repertoire of murinized internalin of listeria monocytogenes |
| topic | Listeria monocytogenes Murinized InlA Murine N-cadherin Human N-cadherin Protein-protein interactions UCTD |
| url | http://hdl.handle.net/2263/56248 |