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Continuous production of succinic acid with Actinobacillus succinogenes biofilms: Effect of complex nitrogen source on yield and productivity
Dissertation (MSc)--University of Pretoria, 2016.
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| Format: | Thesis |
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University of Pretoria
2016
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| _version_ | 1867613612782125056 |
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| access_status_str | Open Access |
| author2 | Nicol, Willie |
| author_browse | Nicol, Willie |
| author_facet | Nicol, Willie |
| collection | Thesis |
| dc_rights_str_mv | © 2016 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2016. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/56458 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:38:54.752Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/56458 Continuous production of succinic acid with Actinobacillus succinogenes biofilms: Effect of complex nitrogen source on yield and productivity Nicol, Willie Vijayan, Uma Rajendra Prasad A. Succinogenes Continuous fermentation Corn steep liquor Biofilm Yeast extract UCTD Engineering, built environment and information technology theses SDG-08 Engineering, built environment and information technology theses SDG-09 Engineering, built environment and information technology theses SDG-12 Dissertation (MSc)--University of Pretoria, 2016. Continuous fermentations were performed in an external-recycle, biofilm reactor using glucose and CO2 as carbon substrates. The nitrogen source for the auxotrophic Actinobacillus succinogenes was a combination of yeast extract (YE) and corn steep liquor (CSL), and sometimes only YE or CSL was used. In this study, the succinic acid productivity of A. succinogenes decreased by 67% as the amount of YE in the complex nitrogen source mixture decreased from 16 g·L-1 to 0 g·L-1. Succinic acid production increased as the CSL concentration in the nitrogen source increased, and the mass ratio of succinic acid to acetic acid exceeded the theoretical maximum limit of 3,93 g·g-1 when only CSL was used as the nitrogen source. The mass ratio of formic acid to acetic acid was consistently within the theoretical yield limitations (0,77 g·g−1) and decreased as the CSL concentration in the nitrogen source increased. The highest SA concentration in this study was 22,57 g·L-1 when only YE was used as the nitrogen source in the growth medium, and the highest SA productivity obtained in this study was 1,58 g·L-1·h-1 when a combination of YE and CSL was used as a nitrogen source. The highest mass ratio of SA to AA achieved was 8,3 g·g-1 when CSL was the sole nitrogen source. The mass ratio of FA to AA was consistently less than 0,77 g·g-1, approaching 0 g·g-1, as the CSL concentration in the nitrogen source increased. National Research Foundation (NRF) mi2026 Chemical Engineering MSc Unrestricted SDG-08: Decent work and economic growth SDG-09: Industry, innovation and infrastructure SDG-12: Responsible consumption and production 2016-08-23T13:56:00Z 2016-08-23T13:56:00Z 2016-09-01 2016 Dissertation Vijayan, URP 2016, Continuous production of succinic acid with Actinobacillus succinogenes biofilms: Effect of complex nitrogen source on yield and productivity, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/56458> S2016 http://hdl.handle.net/2263/56458 © 2016 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | A. Succinogenes Continuous fermentation Corn steep liquor Biofilm Yeast extract UCTD Engineering, built environment and information technology theses SDG-08 Engineering, built environment and information technology theses SDG-09 Engineering, built environment and information technology theses SDG-12 Continuous production of succinic acid with Actinobacillus succinogenes biofilms: Effect of complex nitrogen source on yield and productivity |
| title | Continuous production of succinic acid with Actinobacillus succinogenes biofilms: Effect of complex nitrogen source on yield and productivity |
| title_full | Continuous production of succinic acid with Actinobacillus succinogenes biofilms: Effect of complex nitrogen source on yield and productivity |
| title_fullStr | Continuous production of succinic acid with Actinobacillus succinogenes biofilms: Effect of complex nitrogen source on yield and productivity |
| title_full_unstemmed | Continuous production of succinic acid with Actinobacillus succinogenes biofilms: Effect of complex nitrogen source on yield and productivity |
| title_short | Continuous production of succinic acid with Actinobacillus succinogenes biofilms: Effect of complex nitrogen source on yield and productivity |
| title_sort | continuous production of succinic acid with actinobacillus succinogenes biofilms effect of complex nitrogen source on yield and productivity |
| topic | A. Succinogenes Continuous fermentation Corn steep liquor Biofilm Yeast extract UCTD Engineering, built environment and information technology theses SDG-08 Engineering, built environment and information technology theses SDG-09 Engineering, built environment and information technology theses SDG-12 |
| url | http://hdl.handle.net/2263/56458 |