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Ruminal colonization and potential of Megashaera elsdenii NCIMB 41125 to stimulate rumen development and its effects on the performance of pre-weaned Holstein calves
Thesis (PhD)--University of Pretoria, 2016.
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University of Pretoria
2016
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| access_status_str | Open Access |
| author2 | Erasmus, L.J. (Lourens Jacobus) |
| author_browse | Erasmus, L.J. (Lourens Jacobus) |
| author_facet | Erasmus, L.J. (Lourens Jacobus) |
| collection | Thesis |
| dc_rights_str_mv | © 2016 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Thesis (PhD)--University of Pretoria, 2016. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/57269 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:40:07.413Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/57269 Ruminal colonization and potential of Megashaera elsdenii NCIMB 41125 to stimulate rumen development and its effects on the performance of pre-weaned Holstein calves Erasmus, L.J. (Lourens Jacobus) claudemuya@yahoo.fr Muya, Claude Mukengela UCTD Megasphaera elsdenii Rumen development Holstein calves Natural and agricultural sciences theses SDG-02 Natural and agricultural sciences theses SDG-12 Natural and agricultural sciences theses SDG-15 Thesis (PhD)--University of Pretoria, 2016. Four studies were conducted to determine the survival of Megasphaera elsdenii NCIMB 41125 in the gastro intestinal tract (GIT) of Holstein calves after an oral dose, and evaluate its effects on ruminal fermentation, rumen development and performance. The objective of the first study was to investigate the survival of the bacteria in the GIT and evaluate effects on rumen fermentation and blood metabolites. The second study evaluated the effects and dosing time on intake, growth and diarrhoea in a conventional calf growth system. The third study evaluated the pre- (d 1-56) and post-weaning (d 56-70) effects of the bacteria in an accelerated growth system on intake, growth and blood beta-hydroxybutyrate (BHBA). The fourth study evaluated the effects on rumen development and fermentation. In experiment one, a higher cell count of M. elsdenii (24 h after dosing) and M. elsdenii NCIMB 41125 (24 and 72 h after dosing) were found in the rumen and the colon after dosing on day 7 and 14. An increase in the proportion of butyrate was observed 72 hours later. In experiment 2, measured for 42 days after birth, dry matter and water intakes were increased (P < 0.05) after dosing with M. elsdenii NCIMB 41125 on days 7 and 14 when compared to control calves. Feed efficiency, however, was not affected by dosing. The average numbers of days that diarrhoea was observed were 18 and 4.5 % less in calves dosed on d 7 and d 14, and 11.4 % higher in those dosed on d 21 compared to control calves. In the third study, dosed calves had higher starter DMI compared to control calves (P < 0.01). The opposite was true for milk intake as dosed calves consumed less milk (P = 0.01) than control calves. The total DMI, energy intake and ADG were not affected (P > 0.05), but dosed calves were 5.8 kg heavier (P = 0.01) at weaning and had greater gain:feed ratio (P < 0.01) compared to control calves. The plasma BHBA concentration after dosing was greater for dosed calves (P = 0.02) compared to control calves. After weaning, dosed calves consumed more (P < 0.05) feed and energy, gained 0.37 kg more weight per day (P < 0.01), and weighed 11.6 kg more (P < 0.001) at the end of the study compared to control calves. In the last experiment, dosed calves had a higher reticulo-rumen weight (P = 0.01), papillae width (P < 0.001) and papillae density (P = 0.02) compared to control calves. The rumen wall thickness and papillae length were not affected by dosing. Total VFA s, acetate and propionate were also not affected, but butyrate concentration was higher in dosed compared to control calves (P < 0.04). These results indicate that M. elsdenii NCIMB 411 can colonize and establish in the GIT of pre-weaned calves after a single dose of the bacteria. The increased starter intake, plasma BHBA, and improved rumen fermentation and development in dosed calves suggest greater metabolic activity of the rumen epithelium. Benefit of dosing on feed efficiency was observed on calves in accelerate growth and early weaning. tm2016 bs2026 Animal and Wildlife Sciences PhD Unrestricted SDG-02: Zero hunger SDG-12: Responsible consumption and production SDG-15: Life on land 2016-10-14T07:32:58Z 2016-10-14T07:32:58Z 2016-09-01 2016 Thesis Muya, CM 2016, Ruminal colonization and potential of Megashaera elsdenii NCIMB 41125 to stimulate rumen development and its effects on the performance of pre-weaned Holstein calves, PhD Thesis, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/57269> S2016 http://hdl.handle.net/2263/57269 en © 2016 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Megasphaera elsdenii Rumen development Holstein calves Natural and agricultural sciences theses SDG-02 Natural and agricultural sciences theses SDG-12 Natural and agricultural sciences theses SDG-15 Ruminal colonization and potential of Megashaera elsdenii NCIMB 41125 to stimulate rumen development and its effects on the performance of pre-weaned Holstein calves |
| title | Ruminal colonization and potential of Megashaera elsdenii NCIMB 41125 to stimulate rumen development and its effects on the performance of pre-weaned Holstein calves |
| title_full | Ruminal colonization and potential of Megashaera elsdenii NCIMB 41125 to stimulate rumen development and its effects on the performance of pre-weaned Holstein calves |
| title_fullStr | Ruminal colonization and potential of Megashaera elsdenii NCIMB 41125 to stimulate rumen development and its effects on the performance of pre-weaned Holstein calves |
| title_full_unstemmed | Ruminal colonization and potential of Megashaera elsdenii NCIMB 41125 to stimulate rumen development and its effects on the performance of pre-weaned Holstein calves |
| title_short | Ruminal colonization and potential of Megashaera elsdenii NCIMB 41125 to stimulate rumen development and its effects on the performance of pre-weaned Holstein calves |
| title_sort | ruminal colonization and potential of megashaera elsdenii ncimb 41125 to stimulate rumen development and its effects on the performance of pre weaned holstein calves |
| topic | UCTD Megasphaera elsdenii Rumen development Holstein calves Natural and agricultural sciences theses SDG-02 Natural and agricultural sciences theses SDG-12 Natural and agricultural sciences theses SDG-15 |
| url | http://hdl.handle.net/2263/57269 |