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Drought-induced modulation of the Camellia sinensis metabolome
Dissertation (MSc)--University of Pretoria, 2016.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2016
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| _version_ | 1867613698817785856 |
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| access_status_str | Open Access |
| author2 | Apostolides, Zeno |
| author_browse | Apostolides, Zeno |
| author_facet | Apostolides, Zeno |
| collection | Thesis |
| dc_rights_str_mv | © 2016 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2016. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/57279 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:40:17.184Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/57279 Drought-induced modulation of the Camellia sinensis metabolome Apostolides, Zeno cnyarukowa@gmail.com Nyarukowa, Christopher UCTD Dissertation (MSc)--University of Pretoria, 2016. Climate change is causing droughts affecting crop production on a global scale. Classical breeding and selection strategies for drought tolerant cultivars will help prevent crop losses. Plant breeders, for all crops, need a simple and reliable method to identify drought-tolerant cultivars, but such a method is missing. Plant metabolism is often disrupted by abiotic stress conditions. To survive drought, plants reconfigure their metabolic pathways. Studies have documented the importance of metabolic regulation, i.e. osmolyte accumulation such as polyols and sugars (mannitol, sorbitol); amino acids (proline) during drought. This study identified and quantified metabolites in drought tolerant and drought susceptible Camellia sinensis cultivars under wet and drought stress conditions. For analyses, GC-MS and LC-MS were employed for metabolomics analysis. %RWC results show how the two drought tolerant and two drought susceptible cultivars differed significantly (p ? 0.05) from one another; the drought susceptible exhibited rapid water loss compared to the drought tolerant. There was a significant variation (p < 0.05) in metabolite content (amino acid, sugars) between drought tolerant and drought susceptible tea cultivars after short-time withering conditions. These metabolite changes were similar to those seen in other plant species under drought conditions, thus validating this method. The Short-time Withering Assessment of Probability for Drought Tolerance (SWAPDT) method presented here provides an easy method to identify drought tolerant tea cultivars that will mitigate the effects of drought due to climate change on crop losses. Some of the results presented in this dissertation have been published in the Journal of plant physiology in April 2016. The article is found in Appendix A. tm2016 Biochemistry MSc Unrestricted 2016-10-14T07:33:01Z 2016-10-14T07:33:01Z 2016-09-01 2016 Dissertation Nyarukowa, CT 2016, Drought-induced modulation of the Camellia sinensis metabolome, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/57279> S2016 http://hdl.handle.net/2263/57279 en © 2016 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Drought-induced modulation of the Camellia sinensis metabolome |
| title | Drought-induced modulation of the Camellia sinensis metabolome |
| title_full | Drought-induced modulation of the Camellia sinensis metabolome |
| title_fullStr | Drought-induced modulation of the Camellia sinensis metabolome |
| title_full_unstemmed | Drought-induced modulation of the Camellia sinensis metabolome |
| title_short | Drought-induced modulation of the Camellia sinensis metabolome |
| title_sort | drought induced modulation of the camellia sinensis metabolome |
| topic | UCTD |
| url | http://hdl.handle.net/2263/57279 |