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The effect of delayed evisceration on the microbiological safety of black wildebeest (Connochaetes gnou) meat
Dissertation (MSc)--University of Pretoria, 2016.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2016
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| _version_ | 1867613459791740928 |
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| access_status_str | Open Access |
| author2 | Jansen van Vuuren, M. |
| author_browse | Jansen van Vuuren, M. |
| author_facet | Jansen van Vuuren, M. |
| collection | Thesis |
| dc_rights_str_mv | © 2016 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2016. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/57303 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:36:29.578Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/57303 The effect of delayed evisceration on the microbiological safety of black wildebeest (Connochaetes gnou) meat Jansen van Vuuren, M. casper.vanheerden@ionline.co.za Van Heerden, Casper Vorster UCTD Dissertation (MSc)--University of Pretoria, 2016. Slaughter protocols for game harvesters are defined by the Department of Agriculture, Forestry and Fisheries in South Africa in Veterinary Procedural Notice (VPN 8). VPN 8 stipulates that game animals should normally be eviscerated in a hanging position within 2 hrs after being shot, and if bloating occurs evisceration must be done as an emergency, within 0.5 hr, or as soon as possible after bleeding by the game harvester. It would be beneficial to know if the concept of delayed evisceration has any deleterious effects on the microbiological safety of harvested black wildebeest (Connochaetes gnou) meat. The objective of this research project was to determine if delayed evisceration has an effect on enteric bacterial translocation across the intestinal mucosal barrier into the abdominal cavity. Sixteen harvested animals were grouped together in groups A, B, C, D and E, based on a time delay in evisceration. After each animal was eviscerated, an 8mm punch biopsy from the abdominal peritoneum was collected and placed into a 2 ml Biofreeze vial. In the laboratory, the biopsy samples were inoculated on blood and MacConkey agar plates and incubated for three days at 37°C. The bacterial species targeted for isolation were indicator bacteria for movement across the intestinal barrier. The quantifiable absence of bacteria from the samples that were subjected to bacterial isolation in this study indicates that bacterial translocation across the intestinal barrier is negligible in black wildebeest (Connochaetes gnou) exposed to delays in evisceration for up to 5 hrs. Evidence indicating that black wildebeest (Connochaetes gnou) meat exposed to bloating and a delay in evisceration is not safe for human consumption due to bacterial translocation is therefore lacking. tm2016 Veterinary Tropical Diseases MSc Unrestricted 2016-10-14T07:33:31Z 2016-10-14T07:33:31Z 2016-09-02 2016 Dissertation Van Heerden, CV 2016, The effect of delayed evisceration on the microbiological safety of black wildebeest (Connochaetes gnou) meat, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/57303> S2016 http://hdl.handle.net/2263/57303 en © 2016 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD The effect of delayed evisceration on the microbiological safety of black wildebeest (Connochaetes gnou) meat |
| title | The effect of delayed evisceration on the microbiological safety of black wildebeest (Connochaetes gnou) meat |
| title_full | The effect of delayed evisceration on the microbiological safety of black wildebeest (Connochaetes gnou) meat |
| title_fullStr | The effect of delayed evisceration on the microbiological safety of black wildebeest (Connochaetes gnou) meat |
| title_full_unstemmed | The effect of delayed evisceration on the microbiological safety of black wildebeest (Connochaetes gnou) meat |
| title_short | The effect of delayed evisceration on the microbiological safety of black wildebeest (Connochaetes gnou) meat |
| title_sort | effect of delayed evisceration on the microbiological safety of black wildebeest connochaetes gnou meat |
| topic | UCTD |
| url | http://hdl.handle.net/2263/57303 |