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Improvement of a liquid phase blocking ELISA for enhanced detection and measurement of antibodies against the SAT3 serotype of FMDV

Dissertation (MSc)--University of Pretoria, 2016.

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Other Authors: Maree, Francois Frederick
Format: Thesis
Language:English
Published: University of Pretoria 2016
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access_status_str Open Access
author2 Maree, Francois Frederick
author_browse Maree, Francois Frederick
author_facet Maree, Francois Frederick
collection Thesis
dc_rights_str_mv © 2016 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.
description Dissertation (MSc)--University of Pretoria, 2016.
format Thesis
id oai:repository.up.ac.za:2263/57304
institution University of Pretoria (South Africa)
language English
last_indexed 2026-06-10T12:36:26.058Z
license_str Other — see source repository
provenance_str_mv Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository
publishDate 2016
publishDateRange 2016
publishDateSort 2016
publisher University of Pretoria
publisherStr University of Pretoria
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source_str UPSpace — University of Pretoria Institutional Repository
spelling oai:repository.up.ac.za:2263/57304 Improvement of a liquid phase blocking ELISA for enhanced detection and measurement of antibodies against the SAT3 serotype of FMDV Maree, Francois Frederick anthory73@yahoo.com.br Dwarka, Rahana M. Anahory, Iolanda Vieira UCTD Dissertation (MSc)--University of Pretoria, 2016. Foot-and-mouth disease virus (FMDV) is the causative agent of one of the most serious infections of cloven-hoofed animals. An outbreak of FMD not only severely decreases livestock productivity, but also impacts on both the local and export trade of susceptible animals and their products. The Southern African Territories (SAT) types 1-3 are endemic to sub-Saharan Africa and display greater intratypic genomic and antigenic variation than the traditional Euro-Asian types. Southern Africa has an abundant wildlife, especially in National Parks and game reserves. Wildlife, particularly African buffalo (Syncerus caffer), is involved in virus maintenance and epidemiology of the disease. In communities within the proximity of National Parks and game reserves, the wildlife-livestock interface presents a challenge and poses difficulty to livestock disease eradication and control in Africa. In this study, the influence of modifications to the reagents has on the specificity, sensitivity and repeatability of a LPBE, used for the detection of antibodies against FMD, was determined. The sensitivity of the LPBE is dependent on the antigen used in the test and the ability of the sera to cross-react with the antigen. The purified and non-purified virus used as antigen and the capture and detector antibodies were prepared and standardized for this purpose. An attempt was made to reduce the subtype-specificity of the LBPE by including antigens from all the relevant SAT3 strains. A total of 515 sera from FMDV exposed cattle in Mpumalanga during 2011-2012; 1398 sera from unexposed cattle obtained during an FMD survey conducted in the Northern Cape, and 286 sera from FMDV vaccinated cattle next to the Kruger National Park (KNP) were tested with the improved ELISA. A statistical analysis was conducted to compare the results obtained with the newly developed ELISA and the current in house ELISA. The new assay has higher sensitivity for detecting antibodies in vaccinated animals compared to the standard LPBE. The test is specific and suitable for detection of antibodies, and plays a key role toward the control of FMD, specific and suitable for identification and typing of all SAT3 serotype across the range of the genetic variations in the SAT3 serotype of FMDV. tm2016 Veterinary Tropical Diseases MSc Unrestricted 2016-10-14T07:33:31Z 2016-10-14T07:33:31Z 2016-09-02 2016 Dissertation Anahory Monjane, IV 2016, Improvement of a Liquid Phase Blocking ELISA for enhanced detection and measurement of antibodies against the SAT 3 serotype of FMDV, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/57304> S2016 http://hdl.handle.net/2263/57304 en © 2016 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria
spellingShingle UCTD
Improvement of a liquid phase blocking ELISA for enhanced detection and measurement of antibodies against the SAT3 serotype of FMDV
title Improvement of a liquid phase blocking ELISA for enhanced detection and measurement of antibodies against the SAT3 serotype of FMDV
title_full Improvement of a liquid phase blocking ELISA for enhanced detection and measurement of antibodies against the SAT3 serotype of FMDV
title_fullStr Improvement of a liquid phase blocking ELISA for enhanced detection and measurement of antibodies against the SAT3 serotype of FMDV
title_full_unstemmed Improvement of a liquid phase blocking ELISA for enhanced detection and measurement of antibodies against the SAT3 serotype of FMDV
title_short Improvement of a liquid phase blocking ELISA for enhanced detection and measurement of antibodies against the SAT3 serotype of FMDV
title_sort improvement of a liquid phase blocking elisa for enhanced detection and measurement of antibodies against the sat3 serotype of fmdv
topic UCTD
url http://hdl.handle.net/2263/57304

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