Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Proteomic assessment of potential in vitro hepatotoxicity models
Thesis (PhD)--University of Pretoria, 2016.
Saved in:
| Other Authors: | |
|---|---|
| Format: | Thesis |
| Published: |
University of Pretoria
2017
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613556286947328 |
|---|---|
| access_status_str | Open Access |
| author2 | Cromarty, Allan Duncan |
| author_browse | Cromarty, Allan Duncan |
| author_facet | Cromarty, Allan Duncan |
| collection | Thesis |
| dc_rights_str_mv | © 2017 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Thesis (PhD)--University of Pretoria, 2016. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/59107 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:38:00.699Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/59107 Proteomic assessment of potential in vitro hepatotoxicity models Cromarty, Allan Duncan Lilley, Kathryn S. Hurrell, Tracey Health sciences theses SDG-03 Health sciences theses SDG-17 Thesis (PhD)--University of Pretoria, 2016. Scientifically credible and valid biological systems are essential in pharmaceutical research and development. Standardizing in vitro preclinical hepatotoxicity is confounded by the diversity of origin of cells and the ability to retain hepatocellular functions. Key determinants of valid hepatotoxicity models are resemblance to primary human hepatocytes (PHHs), adaptability to high-throughput screening and biological applicability. Numerous in vitro models, including immortalized cell lines and hepatocyte-like cells (HLCs) derived from induced pluripotent stem cells (iPSCs), attempt to reflect features of PHH. Additional influencing factors are the mechanical and geometric environment which dictate functionality and suggest a role for spatial organization as a requirement for mimicking PHH. As there is poor correlation between the cellular genome and proteome, assessing the hepatic phenotypes using proteomics is essential to capture functional cellular responses. The aim of this research was to determine proteomic differences between PHHs and differentially cultured and sourced human hepatocyte-derived cell lines or differentiated HLCs. Additionally, hepatocyte models were used to generate non-specific, proteome-wide information associated with exposure to selected known hepatotoxins to identify potential proteomic signatures of hepatotoxicity. em2026 Pharmacology PhD Unrestricted SDG-03: Good health and well-being SDG-17: Partnerships for the goals 2017-02-20T06:32:26Z 2017-02-20T06:32:26Z 2017-05-05 2016 Thesis Hurrell, T 2016, Proteomic assessment of potential in vitro hepatotoxicity models, PhD Thesis, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/59107> A2017 http://hdl.handle.net/2263/59107 © 2017 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Health sciences theses SDG-03 Health sciences theses SDG-17 Proteomic assessment of potential in vitro hepatotoxicity models |
| title | Proteomic assessment of potential in vitro hepatotoxicity models |
| title_full | Proteomic assessment of potential in vitro hepatotoxicity models |
| title_fullStr | Proteomic assessment of potential in vitro hepatotoxicity models |
| title_full_unstemmed | Proteomic assessment of potential in vitro hepatotoxicity models |
| title_short | Proteomic assessment of potential in vitro hepatotoxicity models |
| title_sort | proteomic assessment of potential in vitro hepatotoxicity models |
| topic | Health sciences theses SDG-03 Health sciences theses SDG-17 |
| url | http://hdl.handle.net/2263/59107 |