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The role of cell apoptosis on in vitroproduced beef cattle embryos
Dissertation (MSc (Agric))--University of Pretoria, 2016.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2017
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| _version_ | 1867613542971080704 |
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| access_status_str | Open Access |
| author2 | Lehloenya, Khoboso C. |
| author_browse | Lehloenya, Khoboso C. |
| author_facet | Lehloenya, Khoboso C. |
| collection | Thesis |
| dc_rights_str_mv | © 2017 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Agric))--University of Pretoria, 2016. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/60847 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:37:48.825Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/60847 The role of cell apoptosis on in vitroproduced beef cattle embryos Lehloenya, Khoboso C. u14430097@tuks.co.za Marle-Koster, Este Nedambale, T.L. Nkadimeng, Marble UCTD Beef cattle embryos Oocyte maturation In vitro embryo production Cell apoptosis Natural and agricultural sciences theses SDG-02 Natural and agricultural sciences theses SDG-03 Natural and agricultural sciences theses SDG-12 Dissertation (MSc (Agric))--University of Pretoria, 2016. The in vitro culture environment of cattle embryos can compromise the survival of developing embryos resulting in cell apoptosis. Detection of cell apoptosis is important for determining embryonic quality and reducing embryonic mortality in female animals prior to transfer. In this study the role of cell apoptosis on in vitro embryos was studied, with the focus on oocyte maturation and embryo production, caspase-3 activity and DNA fragmentation. Cow ovaries were collected from local abattoir and a total of 900 COCs were retrieved per week for the study over five-month period (six replicate/per experiments). COCs were randomly allocated to four incubation temperatures (39, 41, 42 and 43 ̊C) for polar body extrusion. Based on maturation results, two preferred temperatures (39 and 41 ̊C) were selected for maturation. Oocytes were subjected to normal subsequent embryonic conditions post maturation. Embryos produced from both maturation temperatures were then examined for embryonic development, caspase-3 activity and DNA fragmentation for evidence of apoptosis. No difference was (P<0.05) observed in embryonic development between oocytes matured at 39 ̊C and 41 ̊C respectively. Blastocysts produced at 39 ̊C showed significantly (P < 0.05) higher nuclei cells compared to those produced from 41 ̊C matured oocytes. A higher (P< 0.05) caspase-3 activity and DNA fragmentation were observed at (2-4 cell) and (≥ 8cell cell) embryos for 41 ̊C matured oocytes compared to 39 ̊C maturation group. A significantly higher caspase-3 activity and DNA fragmentation on blastocyst produced at 41 ̊C matured oocytes were also evident compared to those produced at 39 ̊C. Although embryos produced from oocytes matured at 41 ̊C showed similar developmental capacity to embryos from 39 ̊C, this study showed that a higher incidence of apoptosis can be expected in embryos produced from oocytes matured at a higher (41 ̊C) temperature than 39 ̊C. bs2026 Animal and Wildlife Sciences MSc (Agric) Unrestricted SDG-02: Zero hunger SDG-03: Good health and well-being SDG-12: Responsible consumption and production 2017-06-05T12:12:04Z 2017-06-05T12:12:04Z 2017-04-05 2016 Dissertation Nkadimeng, M 2016, The role of cell apoptosis on in vitroproduced beef cattle embryos, MSc (Agric) Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/60847> A2017 http://hdl.handle.net/2263/60847 en © 2017 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Beef cattle embryos Oocyte maturation In vitro embryo production Cell apoptosis Natural and agricultural sciences theses SDG-02 Natural and agricultural sciences theses SDG-03 Natural and agricultural sciences theses SDG-12 The role of cell apoptosis on in vitroproduced beef cattle embryos |
| title | The role of cell apoptosis on in vitroproduced beef cattle embryos |
| title_full | The role of cell apoptosis on in vitroproduced beef cattle embryos |
| title_fullStr | The role of cell apoptosis on in vitroproduced beef cattle embryos |
| title_full_unstemmed | The role of cell apoptosis on in vitroproduced beef cattle embryos |
| title_short | The role of cell apoptosis on in vitroproduced beef cattle embryos |
| title_sort | role of cell apoptosis on in vitroproduced beef cattle embryos |
| topic | UCTD Beef cattle embryos Oocyte maturation In vitro embryo production Cell apoptosis Natural and agricultural sciences theses SDG-02 Natural and agricultural sciences theses SDG-03 Natural and agricultural sciences theses SDG-12 |
| url | http://hdl.handle.net/2263/60847 |