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Reduction of selenium by Pseudomonas Stutzeri NT-l; Growth reduction and kinetics
Dissertation (MEng)--University of Pretoria, 2017.
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University of Pretoria
2017
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| _version_ | 1867613677854654464 |
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| access_status_str | Open Access |
| author2 | Chirwa, Evans M.N. |
| author_browse | Chirwa, Evans M.N. |
| author_facet | Chirwa, Evans M.N. |
| collection | Thesis |
| dc_rights_str_mv | © 2017 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MEng)--University of Pretoria, 2017. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/62802 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:39:57.392Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/62802 Reduction of selenium by Pseudomonas Stutzeri NT-l; Growth reduction and kinetics Chirwa, Evans M.N. charlotte.elize@gmail.com Wessels, Charlotte Elize Pseudomonas stutzeri NT-I Reduction kinetics Metal reduction Bacterial growth UCTD Engineering, built environment and information technology theses SDG-06 Engineering, built environment and information technology theses SDG-09 Engineering, built environment and information technology theses SDG-12 Dissertation (MEng)--University of Pretoria, 2017. Bioremediation of seleniferous water is gaining more momentum, especially when it comes to bacterial reduction of the selenium oxyanions. More and more bacterial strains that are able to reduce selenium are being isolated. These bacteria need to be studied further to determine whether they are suited for industrial application. In this study, the reduction of Se(VI) to Se(0) by Pseudomonas stutzeri NT-I was examined using batch experiments with the bacteria suspended in MSM. For the determination of the optimum conditions for the growth of the bacteria, the linearized rate during the exponential phase for different conditions were compared. A pH of 7, temperature of 37 ⁰C, salinity of 20 g.L-1 NaCl and initial concentration of 5 mM selenate were found to be the best at promoting growth. To determine the optimum conditions for the reduction of selenium, the amount of Se(0) recovered from the plug after 16 hours of incubation was measured. A pH of 8, temperature of 37 ⁰C and salinity of 5 g.L-1 resulted in the most Se(0) recovered. The kinetics of the reduction of Se(VI) to Se(0) was found to follow the adapted Monod equation. An increase in the initial Se(VI) concentration positively affected the reduction rate indicating that substrate saturation had not yet been reached. One kmax could be fitted to each of the two reactions but not one Ks. It was found that Ks decreased with increasing initial selenate concentration. Visually it can be deduced that inhibition starts playing a role in the reduction of selenate at a concentration of 4 mM. Pseudomonas stutzeri NT-I is an exemplary selenium reducing agent and deserves more attention, not only for industrial application but also in the research world, for further understanding of the complex mechanism behind metal reduction in bacteria. mi2026 Chemical Engineering MEng Unrestricted SDG-06: Clean water and sanitation SDG-09: Industry, innovation and infrastructure SDG-12: Responsible consumption and production 2017-10-13T13:41:26Z 2017-10-13T13:41:26Z 2017-09-08 2017 Dissertation Wessels, CE 2017, Reduction of selenium by Pseudomonas Stutzeri NT-l; Growth reduction and kinetics, MEng Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/62802> S2017 http://hdl.handle.net/2263/62802 © 2017 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Pseudomonas stutzeri NT-I Reduction kinetics Metal reduction Bacterial growth UCTD Engineering, built environment and information technology theses SDG-06 Engineering, built environment and information technology theses SDG-09 Engineering, built environment and information technology theses SDG-12 Reduction of selenium by Pseudomonas Stutzeri NT-l; Growth reduction and kinetics |
| title | Reduction of selenium by Pseudomonas Stutzeri NT-l; Growth reduction and kinetics |
| title_full | Reduction of selenium by Pseudomonas Stutzeri NT-l; Growth reduction and kinetics |
| title_fullStr | Reduction of selenium by Pseudomonas Stutzeri NT-l; Growth reduction and kinetics |
| title_full_unstemmed | Reduction of selenium by Pseudomonas Stutzeri NT-l; Growth reduction and kinetics |
| title_short | Reduction of selenium by Pseudomonas Stutzeri NT-l; Growth reduction and kinetics |
| title_sort | reduction of selenium by pseudomonas stutzeri nt l growth reduction and kinetics |
| topic | Pseudomonas stutzeri NT-I Reduction kinetics Metal reduction Bacterial growth UCTD Engineering, built environment and information technology theses SDG-06 Engineering, built environment and information technology theses SDG-09 Engineering, built environment and information technology theses SDG-12 |
| url | http://hdl.handle.net/2263/62802 |