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Occurrence and characterisation of the seven major Shiga toxin-producing Escherichia coli serotypes from healthy beef cattle in South Africa
Dissertation (MSc)--University of Pretoria, 2017.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2018
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| _version_ | 1867613518624194560 |
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| access_status_str | Open Access |
| author2 | Karama, Musafiri |
| author_browse | Karama, Musafiri |
| author_facet | Karama, Musafiri |
| collection | Thesis |
| dc_rights_str_mv | © 2018 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2017. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/65499 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:37:25.592Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/65499 Occurrence and characterisation of the seven major Shiga toxin-producing Escherichia coli serotypes from healthy beef cattle in South Africa Karama, Musafiri u16398582@tuks.co.za Ombui, J.N. Mainga, Alfred Omwando UCTD Escherichia coli Virulence genes Reservoir species Food microbiology Zoonoses Polymerase chain reaction (PCR) Beef cattle Veterinary science theses SDG-02 Veterinary science theses SDG-03 Veterinary science theses SDG-12 Dissertation (MSc)--University of Pretoria, 2017. Shiga toxin-producing E. coli (STEC) is a food pathogen causing infections characterised by mild watery to severe bloody diarrhea and complications such as the hemolytic uremic syndrome (HUS). Humans acquire STEC through consumption of contaminated foods of animal origin, vegetables and water. Cattle are the main reservoir of STEC. The severity of STEC infections in humans depends on a number of virulence factors encoded in the bacterium’s genome. The seven major STEC serogroups most frequently incriminated in severe human disease outbreaks and HUS worldwide include O157, O45, O103, O111, O121, O145 and, O26, commonly referred to as the "top/big seven". Although STEC has been incriminated in human disease in South Africa, data on the role of played by cattle in human disease and virulence characteristics of cattle STEC are lacking. Therefore, the objectives of this study were to (i) investigate the presence of the seven major STEC serotypes in healthy beef cattle (cow-calf operations) and (ii) characterise isolates by serotype, virulence genes and markers, and antimicrobial resistance profiles. Polymerase chain reaction (PCR) was carried out to identify STEC serotypes (O and H antigens) and characterize the isolates by virulence factors and markers. The disk diffusion technique (Kirby Bauer test) was used to determine the antimicrobial resistance profiles of STEC isolates against a panel of 15 antimicrobials. Five hundred and seventy-eight STEC isolates (N=578), which had been previously recovered from 559 cattle from five beef farms were screened for STEC O26, O45, O103, O111, O121, O145 and O157. Confirmed STEC belonging to serogroups O26, O45, O103, O111, O121, O145 and O157 to isolates were characterised for major virulence genes including stx1, stx2, eaeA and ehxA. Furthermore, 140 isolates were characterised for xiii Shiga toxins (stx) subtypes, plasmid and pathogenicity island-encoded genes, and antimicrobials resistance profiles. es2026 Paraclinical Sciences MSc Unrestricted SDG-02: Zero hunger SDG-03: Good health and well-being SDG-12: Responsible consumption and production 2018-07-13T06:48:23Z 2018-07-13T06:48:23Z 2018/04/20 2017 Dissertation Mainga, AO 2017, Occurrence and characterisation of the seven major Shiga toxin-producing Escherichia coli (STEC) serotypes in healthy beef cattle in South Africa, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/65499> A2018 http://hdl.handle.net/2263/65499 en © 2018 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Escherichia coli Virulence genes Reservoir species Food microbiology Zoonoses Polymerase chain reaction (PCR) Beef cattle Veterinary science theses SDG-02 Veterinary science theses SDG-03 Veterinary science theses SDG-12 Occurrence and characterisation of the seven major Shiga toxin-producing Escherichia coli serotypes from healthy beef cattle in South Africa |
| title | Occurrence and characterisation of the seven major Shiga toxin-producing Escherichia coli serotypes from healthy beef cattle in South Africa |
| title_full | Occurrence and characterisation of the seven major Shiga toxin-producing Escherichia coli serotypes from healthy beef cattle in South Africa |
| title_fullStr | Occurrence and characterisation of the seven major Shiga toxin-producing Escherichia coli serotypes from healthy beef cattle in South Africa |
| title_full_unstemmed | Occurrence and characterisation of the seven major Shiga toxin-producing Escherichia coli serotypes from healthy beef cattle in South Africa |
| title_short | Occurrence and characterisation of the seven major Shiga toxin-producing Escherichia coli serotypes from healthy beef cattle in South Africa |
| title_sort | occurrence and characterisation of the seven major shiga toxin producing escherichia coli serotypes from healthy beef cattle in south africa |
| topic | UCTD Escherichia coli Virulence genes Reservoir species Food microbiology Zoonoses Polymerase chain reaction (PCR) Beef cattle Veterinary science theses SDG-02 Veterinary science theses SDG-03 Veterinary science theses SDG-12 |
| url | http://hdl.handle.net/2263/65499 |