Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Effect of Tritrichomonas foetus infection on the vaginal bacterial microbiota of heifers
Dissertation (MSc)--University of Pretoria, 2017.
Saved in:
| Other Authors: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
University of Pretoria
2018
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613661294493696 |
|---|---|
| access_status_str | Open Access |
| author2 | Irons, Pete Charles |
| author_browse | Irons, Pete Charles |
| author_facet | Irons, Pete Charles |
| collection | Thesis |
| dc_rights_str_mv | © 2018 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2017. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/65510 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:39:41.715Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/65510 Effect of Tritrichomonas foetus infection on the vaginal bacterial microbiota of heifers Irons, Pete Charles u14451001@tuks.co.za Swanevelder, Zacharias Hendrik Salles-Martins, Isabela UCTD Veterinary science theses SDG-02 Veterinary science theses SDG-03 Veterinary science theses SDG-12 Dissertation (MSc)--University of Pretoria, 2017. Trichomonosis is a venereal disease responsible for extensive economic losses. Tritrichomonas foetus, deposited during copulation, adheres to the vaginal mucosa where it proliferates. The vaginal bacterial microbiota protects the mucosa by colonization resistance. We sought to describe the bovine vaginal microbiota using metagenomics; contrasting between oestrus and dioestrus; and the interaction between the pathogen and the resident bacterial community. Six heifers were divided into control and infected groups. Using endometrial guarded swabs, three cranial vaginal samples were taken on days 1, 11 and 21 of their reproductive cycle, totalling eighteen samples. The DNA of the samples were extracted and the V3-V4 region of the 16S rRNA gene amplified prior to next-generation sequencing, which provided over a million high-quality reads. The vaginal bacterial microbiota of heifers were populated by organisms of the phyla Tenericutes, Proteobacteria, Firmicutes, Actinobacteria and Bacteriodetes. The control oestrus heifers were dominated by the phyla Tenericutes and Proteobacteria, represented by the families Mycoplasmataceae and Pasteurellaceae, respectively. The Shannon entropy (alpha diversity) were higher in oestrus than in dioestrus. Analysis of beta diversity via PERMANOVA did not reveal significant differences between heifers in oestrus and in dioestrus. However, the abundance of the family Mycoplasmataceae appeared to be affected by cycle stage (p<0.05). Analysis of the microbiota in the T. foetus infected heifers revealed a significantly higher alpha diversity compared to the controls in the corresponding stages of the oestrus cycle. Analysis of beta diversity via PERMANOVA revealed significant (p<0.1) differences between the two sample groups. The infected groups were found to have significantly less Mycoplasmataceae, and an increase in the families Bacillaceae, Ruminococcaceae, Propionibacteria, Lachnospiraceae, Paenibacillaceae and Prevotellaceae. The use of a culture-independent method expanded the knowledge of, and illustrated the substantial influence of, a few organisms on the bovine vaginal microbiota. The sex hormones may have influenced the microbiota. The introduction of T. foetus caused a dramatic change to the vaginal microbiota and an increase in the bacterial diversity. es2025 Production Animal Studies MSc Unrestricted SDG-02: Zero hunger SDG-03: Good health and well-being SDG-12: Responsible consumption and production 2018-07-13T06:48:24Z 2018-07-13T06:48:24Z 2018/04/20 2017 Dissertation Salles-Martins, I 2017, Effect of Tritrichomonas foetus infection on the vaginal bacterial microbiota of heifers, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/65510> A2018 http://hdl.handle.net/2263/65510 en © 2018 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Veterinary science theses SDG-02 Veterinary science theses SDG-03 Veterinary science theses SDG-12 Effect of Tritrichomonas foetus infection on the vaginal bacterial microbiota of heifers |
| title | Effect of Tritrichomonas foetus infection on the vaginal bacterial microbiota of heifers |
| title_full | Effect of Tritrichomonas foetus infection on the vaginal bacterial microbiota of heifers |
| title_fullStr | Effect of Tritrichomonas foetus infection on the vaginal bacterial microbiota of heifers |
| title_full_unstemmed | Effect of Tritrichomonas foetus infection on the vaginal bacterial microbiota of heifers |
| title_short | Effect of Tritrichomonas foetus infection on the vaginal bacterial microbiota of heifers |
| title_sort | effect of tritrichomonas foetus infection on the vaginal bacterial microbiota of heifers |
| topic | UCTD Veterinary science theses SDG-02 Veterinary science theses SDG-03 Veterinary science theses SDG-12 |
| url | http://hdl.handle.net/2263/65510 |