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CRISPR/Cas9 mediated deletion of genes encoding putative cell cycle regulators in Plasmodium falciparum
Dissertation (MSc)--University of Pretoria, 2017.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2018
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| _version_ | 1867613568729350145 |
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| access_status_str | Open Access |
| author2 | Birkholtz, Lyn-Marie |
| author_browse | Birkholtz, Lyn-Marie |
| author_facet | Birkholtz, Lyn-Marie |
| collection | Thesis |
| dc_rights_str_mv | © 2018 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2017. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/65954 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:38:13.361Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/65954 CRISPR/Cas9 mediated deletion of genes encoding putative cell cycle regulators in Plasmodium falciparum Birkholtz, Lyn-Marie hildevg@live.co.za Niemand, Jandeli Von Grüning, Hilde UCTD Dissertation (MSc)--University of Pretoria, 2017. Functional genomic tools can be used to interrogate unique features of parasite biology that could be marked for future intervention strategies. One such unique biological feature is the atypical cell cycle of the Plasmodium falciparum parasite, with a particular interest in the key regulators that modulate the parasite’s cell cycle progression. The orderly progression of the cell cycle of the parasite allows it to undergo a vast expansion of parasite numbers during a malaria infection and cause pathologies in infected individuals. However, the precise control mechanisms and functional cascades involved in the P. falciparum parasite’s unusual cell cycle have not yet been fully elucidated. A recent study showed that gene expression profiles of the parasite switch between states of cell cycle arrest (quiescence) to cell cycle re-entry (proliferation). Global transcriptome expression data from this study was used to identify potential cell cycle regulators. The study identified a number of putative cell cycle regulators and their predicted functional interacting partners, particularly transcription factors and several members of the origin of replication complex. The study subsequently aimed to functionally validate these putative regulators in a reverse genetics approach by creating targeted disruption of the putative regulatory genes using the CRISPR-Cas9 system. Preliminary knockout studies indicated possible essential phenotypes of these putative cell cycle regulators in P. falciparum parasites. Given that cell cycle elements of the P. falciparum parasites are unique and divergent from those of the human host, the information provided in this study dissects the role of regulators in cell cycle modulation in the parasite. Not only is this important to understand parasite biology, but these cell cycle regulators are attractive potential sites for chemical interference of parasite proliferation and thereby provide as novel drug targets for antimalarial discoveries. Biochemistry MSc Unrestricted 2018-07-25T09:01:11Z 2018-07-25T09:01:11Z 2018/04/24 2017 Dissertation Von Grüning, H 2017, CRISPR/Cas9 mediated deletion of genes encoding putative cell cycle regulators in Plasmodium falciparum, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/65954> A2018 http://hdl.handle.net/2263/65954 en © 2018 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD CRISPR/Cas9 mediated deletion of genes encoding putative cell cycle regulators in Plasmodium falciparum |
| title | CRISPR/Cas9 mediated deletion of genes encoding putative cell cycle regulators in Plasmodium falciparum |
| title_full | CRISPR/Cas9 mediated deletion of genes encoding putative cell cycle regulators in Plasmodium falciparum |
| title_fullStr | CRISPR/Cas9 mediated deletion of genes encoding putative cell cycle regulators in Plasmodium falciparum |
| title_full_unstemmed | CRISPR/Cas9 mediated deletion of genes encoding putative cell cycle regulators in Plasmodium falciparum |
| title_short | CRISPR/Cas9 mediated deletion of genes encoding putative cell cycle regulators in Plasmodium falciparum |
| title_sort | crispr cas9 mediated deletion of genes encoding putative cell cycle regulators in plasmodium falciparum |
| topic | UCTD |
| url | http://hdl.handle.net/2263/65954 |