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Effect of antimicrobial usage on enteric bacterial populations with focus on virulence and resistance profiles of Escherichia coli in growing pigs
Dissertation (MSc)--University of Pretoria, 2018.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2018
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| _version_ | 1867613504876314624 |
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| access_status_str | Open Access |
| author2 | Fasina, Folorunso Oludayo |
| author_browse | Fasina, Folorunso Oludayo |
| author_facet | Fasina, Folorunso Oludayo |
| collection | Thesis |
| dc_rights_str_mv | � 2018 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2018. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/67746 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:37:12.475Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/67746 Effect of antimicrobial usage on enteric bacterial populations with focus on virulence and resistance profiles of Escherichia coli in growing pigs Fasina, Folorunso Oludayo rukkky03@yahoo.com Madoroba, Evelyn Abubakar, Rukayya Hussain UCTD Unrestricted Escherichia coli Pigs -- Diseases Antibiotics Veterinary science theses SDG-01 SDG-01: No poverty Dissertation (MSc)--University of Pretoria, 2018. Microbial community in the gut of pigs provides a vast and complex microbial network of community diversity important for its health and development. Pathogenic Escherichia coli are responsible for acute profuse diarrhoea with resultant high morbidity and mortality. Antibiotics are used as growth promoters and for therapeutic purposes in pigs. Misuse, abuse and overuse of these antibiotics have led to development of resistant bacterial strains. This study reports the effect of antimicrobial usage on frequency in which growing pigs habour ETEC and VTEC virulence genes and compared phenotypic and genotypic antibiotic resistance pattern of E. coli and metagenomics analysis of fecal samples collected from; (i) pigs receiving normal farm treatment without antibiotics usage, over a 70 day period, and (ii) pigs allowed treatment with antibiotics and monitored over a 70 day period. Our hypothesis was that the use of antibiotics in commercial pig farms affect gut microbial population. A total of 241 E.coli strains were isolated and antibiotics resistance testing through disk diffusion and PCR was conducted. Sequencing was also done using the Miseq Illumina platform. Virulence genes were detected in [24.8% (Cl95%: 18.2-32.7)] of the antibiotic group isolates and [43.5% (Cl95%: 34.5-52.9)] of the non antibiotic group with a significant difference (P=0.002). Phenotypic resistance to oxytetracycline was most common and were significant (P = 0.03) in samples of days 10 (P = 0.02) and 21 (P = 0.01). Furthermore, [63.9% (Cl95%: 57.6, 69.7)] possesed one or more of the four tested tetracycline resistance genes. Significant statistical difference exists in bacterial structure and composition in the gut of growing pigs P<0.05. Firmicutes, Bacteriotedes and Proteobacteria were the three most abundant phyla and composition was statistically significant during the growing period. The study showed that antibiotics usage increases gut bacterial population in growing pigs. Disease causing virulence genes and antibiotics resistance genes may occur even without antibiotics usage in growing pigs and other factors may be involved. Veterinary Tropical Diseases MSc 2018-12-05T08:04:48Z 2018-12-05T08:04:48Z 2009/03/18 2018 Dissertation Abubakar, RH 2018, Effect of antimicrobial usage on enteric bacterial populations with focus on virulence and resistance profiles of Escherichia coli in growing pigs, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/67746> S2018 http://hdl.handle.net/2263/67746 en � 2018 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Unrestricted Escherichia coli Pigs -- Diseases Antibiotics Veterinary science theses SDG-01 SDG-01: No poverty Effect of antimicrobial usage on enteric bacterial populations with focus on virulence and resistance profiles of Escherichia coli in growing pigs |
| title | Effect of antimicrobial usage on enteric bacterial populations with focus on virulence and resistance profiles of Escherichia coli in growing pigs |
| title_full | Effect of antimicrobial usage on enteric bacterial populations with focus on virulence and resistance profiles of Escherichia coli in growing pigs |
| title_fullStr | Effect of antimicrobial usage on enteric bacterial populations with focus on virulence and resistance profiles of Escherichia coli in growing pigs |
| title_full_unstemmed | Effect of antimicrobial usage on enteric bacterial populations with focus on virulence and resistance profiles of Escherichia coli in growing pigs |
| title_short | Effect of antimicrobial usage on enteric bacterial populations with focus on virulence and resistance profiles of Escherichia coli in growing pigs |
| title_sort | effect of antimicrobial usage on enteric bacterial populations with focus on virulence and resistance profiles of escherichia coli in growing pigs |
| topic | UCTD Unrestricted Escherichia coli Pigs -- Diseases Antibiotics Veterinary science theses SDG-01 SDG-01: No poverty |
| url | http://hdl.handle.net/2263/67746 |