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Clinical signs synovial fluid cytology and growth factor concentrations after intra-articular use of a platelet-rich product in horses with osteoarthritis
Dissertation (MMedVet)--University of Pretoria, 2017.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2018
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| _version_ | 1867613500926328832 |
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| access_status_str | Open Access |
| author2 | Goddard, Amelia |
| author_browse | Goddard, Amelia |
| author_facet | Goddard, Amelia |
| collection | Thesis |
| dc_rights_str_mv | © 2018 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MMedVet)--University of Pretoria, 2017. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/67909 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:37:08.629Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/67909 Clinical signs synovial fluid cytology and growth factor concentrations after intra-articular use of a platelet-rich product in horses with osteoarthritis Goddard, Amelia yolandi.smit@up.ac.za Marais, Hendrik J. Smit, Yolandi Unrestricted UCTD Dissertation (MMedVet)--University of Pretoria, 2017. Background: Osteoarthritis (OA) is a common cause of lameness in equines, resulting in poor performance. Platelet-rich plasma (PRP) can deliver a collection of bioactive molecules that play important roles in conditions such as OA. Objectives: To (1) investigate the clinical and synovial changes, (2) determine serum amyloid A (SAA) and total protein (TP) concentrations on serum and synovial fluid, and (3) determine synovial fluid platelet-derived growth factor-BB (PDGF-BB) and transforming growth factor-B1 (TGF-b1) in normal and osteoarthritic joints after intra-articular injection with a platelet-rich product (PRPr). Study design: Experimental. Methods: Horses (n = 5) with naturally occurring OA, affecting either antebrachiocarpal, middle carpal or metacarpophalangeal joints, and normal horses (n = 5). A PRPr was prepared from all horses using a gravity filtration system. Clinical evaluation, synovial fluid analysis, and determination of serum and synovial SAA and TP concentrations were performed on day 0 (prior to PRPr treatment), day 1, day 2, day 5, day 21 and day 56 posttreatment. Synovial fluid growth factor concentrations (PDGF-BB and TGF-β1) were determined on day 1 and day 5. PRPr composition was also analysed. Results: The gravity filtration system produced a moderately concentrated PRPr. The synovial effusion score was statistically significant between the control and OA group on day 0 (p < 0.05) with a higher score in the OA group. However, within the control group the synovial effusion score was significantly elevated on day 1 and 2 compared to day 0 (p < 0.05). For both groups the synovial fluid nucleated cell count, predominantly intact neutrophils, were significantly increased on day 1 and day 2 (p < 0.001 for both), with no significant difference between groups. The mean PDGF-BB and TGF-β1 concentrations were high for both groups but significantly lower in the OA group (p < 0.01 and p < 0.001, respectively) on day 1 compared to normal joints. Concentrations for PDGF-BB remained similar on day 5, compared to day 1, with no significant difference between groups. Conclusions: Intra-articular treatment with PRPr resulted in a transient synovial inflammatory reaction of short duration. Synovial fluid growth factor concentrations were elevated after intra-articular injection of PRPr in OA joints. Companion Animal Clinical Studies MMedVet Unrestricted 2018-12-05T08:05:56Z 2018-12-05T08:05:56Z 2009/06/18 2017 Dissertation Smit, Y 2017, Clinical signs synovial fluid cytology and growth factor concentrations after intra-articular use of a platelet-rich product in horses with osteoarthritis, MMedVet Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/67909> S2018 http://hdl.handle.net/2263/67909 en © 2018 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Unrestricted UCTD Clinical signs synovial fluid cytology and growth factor concentrations after intra-articular use of a platelet-rich product in horses with osteoarthritis |
| title | Clinical signs synovial fluid cytology and growth factor concentrations after intra-articular use of a platelet-rich product in horses with osteoarthritis |
| title_full | Clinical signs synovial fluid cytology and growth factor concentrations after intra-articular use of a platelet-rich product in horses with osteoarthritis |
| title_fullStr | Clinical signs synovial fluid cytology and growth factor concentrations after intra-articular use of a platelet-rich product in horses with osteoarthritis |
| title_full_unstemmed | Clinical signs synovial fluid cytology and growth factor concentrations after intra-articular use of a platelet-rich product in horses with osteoarthritis |
| title_short | Clinical signs synovial fluid cytology and growth factor concentrations after intra-articular use of a platelet-rich product in horses with osteoarthritis |
| title_sort | clinical signs synovial fluid cytology and growth factor concentrations after intra articular use of a platelet rich product in horses with osteoarthritis |
| topic | Unrestricted UCTD |
| url | http://hdl.handle.net/2263/67909 |