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Comparing a modified E. coli phytase to other heat stable phytase products by evaluating growth performance and leg health in broilers
Dissertation (MSc (Agric))--University of Pretoria, 2017.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2018
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| _version_ | 1867613539518119936 |
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| access_status_str | Open Access |
| author2 | Nkukwana, Thobela T. |
| author_browse | Nkukwana, Thobela T. |
| author_facet | Nkukwana, Thobela T. |
| collection | Thesis |
| dc_rights_str_mv | © 2018 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Agric))--University of Pretoria, 2017. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/67910 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:37:45.339Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/67910 Comparing a modified E. coli phytase to other heat stable phytase products by evaluating growth performance and leg health in broilers Nkukwana, Thobela T. hunirose@gmail.com Solomon, Hanaa Unrestricted UCTD Phosphorus Enzymes Performance efficiency Bone mineralisation Ash Natural and agricultural sciences theses SDG-02 Natural and agricultural sciences theses SDG-03 Natural and agricultural sciences theses SDG-12 Dissertation (MSc (Agric))--University of Pretoria, 2017. The objective of this study was to determine the optimal dose of the new generation modified E.coli 6-phytase (modified E.coli) that was developed using gene site saturation mutagenesis, and to compare its efficacy to current heat-stable phytases under typical South African commercial broiler production conditions. A total of 2,340 as-hatched Cobb 500 chicks were randomly allocated to nine treatment groups, each replicated 13 times with 20 chicks per pen. The first three treatments consisted of two negative control diets and a positive control diet as follows: negative control (NC1) – starter diet with 0.23% available phosphorus (avP); NC2 – starter diet with 0.33% avP; positive control (PC) – starter diet with 0.43% avP. For each control, the finisher diets had a 0.11% reduction in avP. The remaining six treatments consisted of NC1 supplemented with three different phytase products (modified E.coli, product X and product Y) at two different doses for each product (500 FTU/kg diet and 1,000 FTU/kg diet). Production performance parameters, bone strength, bone ash content and bone mineral content were evaluated. Broilers fed the NC1 diet showed a significantly lower (P<0.05) body weight, feed conversion ratio (FCR), production efficiency factor (PEF), bone strength and ash compared to all other treatment diets. All phytase-supplemented treatments showed results similar to the PC diet (P>0.05). No significant differences were detected among phytase-supplemented treatments. Numerically (P>0.05), broilers fed the diet supplemented with modified E.coli at 500FTU/kg had the greatest body weight, cumulative feed intake and PEF values, and the lowest FCR values on day 35 compared to all other phytase-supplemented diets. Numerically, in terms of bone development broilers fed a diet containing phytase at 1,000FTU/kg had the highest bone breaking strength compared to their 500FTU/kg counterparts, with modified E.coli numerically scoring the highest bone breaking strength, ash and phosphorous deposition, followed by product X and then product Y. In conclusion, broiler performance using this new generation modified E.coli 6-phytase product is comparable to other phytase products on the South African market at an optimal dose of 500FTU/kg diet. bs2026 Animal and Wildlife Sciences MSc (Agric) Unrestricted SDG-02: Zero hunger SDG-12: Responsible consumption and production SDG-12: Responsible consumption and production 2018-12-05T08:05:56Z 2018-12-05T08:05:56Z 2009/04/18 2017 Dissertation Solomon, H 2017, Comparing a modified E. coli phytase to other heat stable phytase products by evaluating growth performance and leg health in broilers, MSc (Agric) Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/67910> S2018 http://hdl.handle.net/2263/67910 en © 2018 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Unrestricted UCTD Phosphorus Enzymes Performance efficiency Bone mineralisation Ash Natural and agricultural sciences theses SDG-02 Natural and agricultural sciences theses SDG-03 Natural and agricultural sciences theses SDG-12 Comparing a modified E. coli phytase to other heat stable phytase products by evaluating growth performance and leg health in broilers |
| title | Comparing a modified E. coli phytase to other heat stable phytase products by evaluating growth performance and leg health in broilers |
| title_full | Comparing a modified E. coli phytase to other heat stable phytase products by evaluating growth performance and leg health in broilers |
| title_fullStr | Comparing a modified E. coli phytase to other heat stable phytase products by evaluating growth performance and leg health in broilers |
| title_full_unstemmed | Comparing a modified E. coli phytase to other heat stable phytase products by evaluating growth performance and leg health in broilers |
| title_short | Comparing a modified E. coli phytase to other heat stable phytase products by evaluating growth performance and leg health in broilers |
| title_sort | comparing a modified e coli phytase to other heat stable phytase products by evaluating growth performance and leg health in broilers |
| topic | Unrestricted UCTD Phosphorus Enzymes Performance efficiency Bone mineralisation Ash Natural and agricultural sciences theses SDG-02 Natural and agricultural sciences theses SDG-03 Natural and agricultural sciences theses SDG-12 |
| url | http://hdl.handle.net/2263/67910 |