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Failure to detect equid herpesvirus type 1 DNA in Thoroughbred placentae and healthy new-born foals
Dissertation (MSc)--University of Pretoria, 2017.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2018
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| _version_ | 1867613602187313152 |
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| access_status_str | Open Access |
| author2 | Brown, Geoffrey James |
| author_browse | Brown, Geoffrey James |
| author_facet | Brown, Geoffrey James |
| collection | Thesis |
| dc_rights_str_mv | � 2018 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2017. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/67946 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:38:45.051Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/67946 Failure to detect equid herpesvirus type 1 DNA in Thoroughbred placentae and healthy new-born foals Brown, Geoffrey James Veterinary science theses SDG-03 larajeanbrown@gmail.com Schulman, Martin L. Brown, Lara Jean UCTD Unrestricted Equid alphaherpesvirus 1 (EHV-1) Equid herpesvirus type 4 (EHV-4) Vertical transmission / transplacental infection Placenta / fetal membranes / foetal membranes Nasopharynx Viral latency foal sampling methods Equine reproduction Veterinary diagnostics Veterinary science theses SDG-02 Veterinary science theses SDG-09 Veterinary science theses SDG-12 Veterinary science theses SDG-15 Dissertation (MSc)--University of Pretoria, 2017. Equid alphaherpesvirus 1 (EHV-1) is an economically important virus, associated with respiratory infection, late gestation abortion, neonatal death and myeloencephalopathy in horses. The aim of the present study was to test the hypothesis that EHV-1 is present in the nasopharynx and placentae of neonatal foals in the absence of clinical signs of infection. This would suggest that vertical transmission of virus occurs in inter-epizootic periods: such information could inform foaling management and the potential eradication of the virus by vaccination. Samples were collected from animals resident on a single farm in the Western Cape Province, South Africa, which had not experienced a clinical outbreak of EHV-1 recently. Sterile swab samples from 71 post-partum Thoroughbred mares, their healthy full-term foals and fetal membranes were obtained and assayed for EHV-1 and EHV-4 nucleic acid using a duplex quantitative polymerase chain reaction (qPCR). The null hypothesis for this study was that EHV-1 was not present in the nasopharynx and placentae of new-born, viable and healthy foals. As no EHV-1 or EHV-4 nucleic acid was detected on a duplex EHV-1/EHV-4 qPCR assay from the mare and foal nasal and fetal membrane swabs, the null hypothesis was accepted. It was therefore concluded that there was no detectable EHV-1 and -4 DNA in this population at the time of sampling. It was speculated that this may have been due to the cyclical nature of EHV-1 infections. The inclusion of additional breeding seasons on additional farms would be valuable for future studies. es2025 Production Animal Studies MSc SDG-02: Zero hunger SDG-03: Good health and well-being SDG-09: Industry, innovation and infrastructure SDG-12: Responsible consumption and production SDG-15: Life on land 2018-12-05T08:06:06Z 2018-12-05T08:06:06Z 2009/06/18 2018 Dissertation Brown, LJ 2018, Failure to detect equid herpesvirus type 1 DNA in Thoroughbred placentae and healthy new-born foals, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/67946> S2018 http://hdl.handle.net/2263/67946 en � 2018 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Unrestricted Equid alphaherpesvirus 1 (EHV-1) Equid herpesvirus type 4 (EHV-4) Vertical transmission / transplacental infection Placenta / fetal membranes / foetal membranes Nasopharynx Viral latency foal sampling methods Equine reproduction Veterinary diagnostics Veterinary science theses SDG-02 Veterinary science theses SDG-09 Veterinary science theses SDG-12 Veterinary science theses SDG-15 Failure to detect equid herpesvirus type 1 DNA in Thoroughbred placentae and healthy new-born foals |
| title | Failure to detect equid herpesvirus type 1 DNA in Thoroughbred placentae and healthy new-born foals |
| title_full | Failure to detect equid herpesvirus type 1 DNA in Thoroughbred placentae and healthy new-born foals |
| title_fullStr | Failure to detect equid herpesvirus type 1 DNA in Thoroughbred placentae and healthy new-born foals |
| title_full_unstemmed | Failure to detect equid herpesvirus type 1 DNA in Thoroughbred placentae and healthy new-born foals |
| title_short | Failure to detect equid herpesvirus type 1 DNA in Thoroughbred placentae and healthy new-born foals |
| title_sort | failure to detect equid herpesvirus type 1 dna in thoroughbred placentae and healthy new born foals |
| topic | UCTD Unrestricted Equid alphaherpesvirus 1 (EHV-1) Equid herpesvirus type 4 (EHV-4) Vertical transmission / transplacental infection Placenta / fetal membranes / foetal membranes Nasopharynx Viral latency foal sampling methods Equine reproduction Veterinary diagnostics Veterinary science theses SDG-02 Veterinary science theses SDG-09 Veterinary science theses SDG-12 Veterinary science theses SDG-15 |
| url | http://hdl.handle.net/2263/67946 |