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A case-control study to evaluate resistance to Haemonchus contortus in SA Dohne Merino sheep
Dissertation (MSc)--University of Pretoria, 2018.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2018
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| _version_ | 1867613714236047360 |
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| access_status_str | Open Access |
| author2 | Visser, Carina |
| author_browse | Visser, Carina |
| author_facet | Visser, Carina |
| collection | Thesis |
| dc_rights_str_mv | © 2018 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2018. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/67992 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:40:31.851Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/67992 A case-control study to evaluate resistance to Haemonchus contortus in SA Dohne Merino sheep Visser, Carina u15414699@tuks.co.za Snyman, M.A. Dlamini, Njabulo Mark UCTD Unrestricted Genetic diversity Animal disease control Livestock health Parasite resistance Genetic selection Natural and agricultural sciences theses SDG-02 Natural and agricultural sciences theses SDG-03 Natural and agricultural sciences theses SDG-15 Dissertation (MSc)--University of Pretoria, 2018. Gastrointestinal parasitism is a major problem to livestock productivity worldwide and small ruminant production is affected the most. Resistance of gastrointestinal nematodes (GIN) to anthelmintics is a widespread problem. Breeding animals resistant to nematode infestation has been proposed as a sustainable alternative. The aim of this study was to use highthroughput genome-wide SNP data to investigate the genetic background of GIN resistance in SA Dohne Merino sheep. The farm Wauldby in the Stutterheim district of South Africa has a history of heavy H. contortus challenge and implemented a selection strategy for resistance to H. contortus in 2011. Faecal egg count (FEC), body condition scores (BCS) and FAMACHA scores (FAM) were recorded on all lambs from weaning in January until the end of June annually. Lambs were only drenched if they had a FAM of 2.5 or higher. Breeding values (EBV) for FEC were estimated for the Wauldby animals born from 2011 to 2014. The GADI Dohne Merino flock is kept at the Grootfontein Agricultural Development Institute and has never been subjected to selection for resistance to GIN. FEC ranged from 0 to 52 500 among animals, recordings and years. Wauldby lambs that were not dosed had an overall lower FEC and FAM and higher BCS than lambs that were dosed once or more. Wauldby animals that were selected for genotyping based on EBV for FEC. Within years, animals with the highest (n= 48) and lowest (n=48) EBV for FEC were selected among the Dosed (Cases), as well as the Not dosed (Controls) (Low EBV FEC (n=52) and High EBV FEC (n=48). DNA obtained from blood samples were genotyped using the Illumina® Ovine SNP50 BeadChip. Principal component analysis (PCA) plot was performed using SNP & Variation Suite (SVS) from Golden Helix. Four distinct genetic clusters were observed, with the GADI Dohne Merino sheep population clustering separately. The Wauldby Dohne Merino population differentiated into 3 distinct clusters. ADMIXTURE version 1.23 was used to investigate population genetic structure. Fixation index (FST) was estimated between genetic clusters and ranged from low to moderate (0.040563 to 0.091004). The genetic diversity between the populations was assessed and observed heterozygosity (Ho), values of 0.3733 ± 0.1341 and 0.3736 ± 0.1468 were observed for the Wauldby Dohne Merino and GADI Dohne Merino sheep populations, respectively. The Wauldby animals in the different clusters were compared. Cluster 3 had lower FEC, lower FAM and higher BCS (P <0.01) compared to the two other genetic clusters. FEC breeding values of 114 ± 97, -629 ± 84 and -2 ± 45 were recorded for Cluster 2, 3 and 4, respectively. The distribution of runs of homozygosity was determined for Clusters 1 to 4. Cluster 4 had the most animals and the highest number of ROH was observed in this genetic cluster. The results from this study indicated that it should be possible to select for resistance to H. contortus on the basis of the phenotypic traits. bs2026 Animal and Wildlife Sciences MSc (Agric) SDG-02: Zero hunger SDG-03: Good health and well-being SDG-15: Life on land 2018-12-05T08:06:23Z 2018-12-05T08:06:23Z 2009/04/18 2018 Dissertation Dlamini, NM 2018, A case-control study to evaluate resistance to Haemonchus contortus in SA Dohne Merino sheep, MSc (Agric) Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/67992> S2018 http://hdl.handle.net/2263/67992 en © 2018 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Unrestricted Genetic diversity Animal disease control Livestock health Parasite resistance Genetic selection Natural and agricultural sciences theses SDG-02 Natural and agricultural sciences theses SDG-03 Natural and agricultural sciences theses SDG-15 A case-control study to evaluate resistance to Haemonchus contortus in SA Dohne Merino sheep |
| title | A case-control study to evaluate resistance to Haemonchus contortus in SA Dohne Merino sheep |
| title_full | A case-control study to evaluate resistance to Haemonchus contortus in SA Dohne Merino sheep |
| title_fullStr | A case-control study to evaluate resistance to Haemonchus contortus in SA Dohne Merino sheep |
| title_full_unstemmed | A case-control study to evaluate resistance to Haemonchus contortus in SA Dohne Merino sheep |
| title_short | A case-control study to evaluate resistance to Haemonchus contortus in SA Dohne Merino sheep |
| title_sort | case control study to evaluate resistance to haemonchus contortus in sa dohne merino sheep |
| topic | UCTD Unrestricted Genetic diversity Animal disease control Livestock health Parasite resistance Genetic selection Natural and agricultural sciences theses SDG-02 Natural and agricultural sciences theses SDG-03 Natural and agricultural sciences theses SDG-15 |
| url | http://hdl.handle.net/2263/67992 |