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Integrative transcriptome and phenome analysis reveal unique regulatory cascades controlling the intraerythrocytic asexual and sexual development of human malaria parasites
Thesis (PhD)--University of Pretoria, 2018.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2019
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| _version_ | 1867613454267842560 |
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| access_status_str | Open Access |
| author2 | Birkholtz, Lyn-Marie |
| author_browse | Birkholtz, Lyn-Marie |
| author_facet | Birkholtz, Lyn-Marie |
| collection | Thesis |
| dc_rights_str_mv | © 2018 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Thesis (PhD)--University of Pretoria, 2018. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/68244 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:36:24.202Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/68244 Integrative transcriptome and phenome analysis reveal unique regulatory cascades controlling the intraerythrocytic asexual and sexual development of human malaria parasites Birkholtz, Lyn-Marie u29094110@tuks.co.za Niemand, Jandeli Llinás, Manuel Van Biljon, Riette Andele UCTD Plasmodium falciparum Malaria Africa Thesis (PhD)--University of Pretoria, 2018. This doctoral study investigates three crucial aspects of malaria parasite development: 1) the atypical cell cycle that allows the rapid proliferation of asexual parasites; 2) the full molecular profile of gametocytogenesis enabling the cellular differentiation that allows the parasite to transmit; and 3) the metabolic differences between these proliferating and differentiating parasites . Here, we contributed a novel system by developing a cell cycle synchronization tool that reversibly blocks the development of asexual parasites at the G1/S transition. This system provided the opportunity to characterize cell cycle phases in the parasite and additionally evaluate molecular mechanisms associated with cell cycle arrest or re-initiation. These analyses indicate the close interaction between transcriptional regulation and signal transduction cascades in the progression through the parasite’s cell cycle and for the first time highlight aspects of controlled cell cycle regulation in Plasmodium. This study also contributes a detailed analysis of the parasite's sexual differentiation that revealed distinct developmental transitions within sexually differentiating parasites and propose possible epigenetic and transcriptional regulators of this process. Finally, a whole cell phenotype microarray system was evaluated for its ability to measure the metabolic processes that define asexual and sexual stage metabolism as a functional consequence of changed gene expression profiles during proliferation and differentiation. Biochemistry PhD Unrestricted 2019-01-25T07:05:26Z 2019-01-25T07:05:26Z 2019-04 2018-12 Thesis Van Biljon, RA 2018, Integrative transcriptome and phenome analysis reveal unique regulatory cascades controlling the intraerythrocytic asexual and sexual development of human malaria parasites, PhD thesis, University of Pretoria. http://hdl.handle.net/2263/68244 en © 2018 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Plasmodium falciparum Malaria Africa Integrative transcriptome and phenome analysis reveal unique regulatory cascades controlling the intraerythrocytic asexual and sexual development of human malaria parasites |
| title | Integrative transcriptome and phenome analysis reveal unique regulatory cascades controlling the intraerythrocytic asexual and sexual development of human malaria parasites |
| title_full | Integrative transcriptome and phenome analysis reveal unique regulatory cascades controlling the intraerythrocytic asexual and sexual development of human malaria parasites |
| title_fullStr | Integrative transcriptome and phenome analysis reveal unique regulatory cascades controlling the intraerythrocytic asexual and sexual development of human malaria parasites |
| title_full_unstemmed | Integrative transcriptome and phenome analysis reveal unique regulatory cascades controlling the intraerythrocytic asexual and sexual development of human malaria parasites |
| title_short | Integrative transcriptome and phenome analysis reveal unique regulatory cascades controlling the intraerythrocytic asexual and sexual development of human malaria parasites |
| title_sort | integrative transcriptome and phenome analysis reveal unique regulatory cascades controlling the intraerythrocytic asexual and sexual development of human malaria parasites |
| topic | UCTD Plasmodium falciparum Malaria Africa |
| url | http://hdl.handle.net/2263/68244 |