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Optimisation of histocompatibility testing in living-donor renal transplantation
Thesis (PhD)--University of Pretoria, 2018.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2019
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| _version_ | 1867613453214023680 |
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| access_status_str | Open Access |
| author2 | Meyer, Pieter |
| author_browse | Meyer, Pieter |
| author_facet | Meyer, Pieter |
| collection | Thesis |
| dc_rights_str_mv | © 2018 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Thesis (PhD)--University of Pretoria, 2018. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/68460 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:36:23.211Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/68460 Optimisation of histocompatibility testing in living-donor renal transplantation Meyer, Pieter luyanda.kwofie@nhls.ac.za Karusseit, V.O.L. Kwofie, Luyanda Laura Illicia HLA cross-match Histocompatibility End-stage kidney diease Allotransplantation Alloantigen T cell alloreactivity Renal transplantation Donor-recipient compatibility Kidney transplant Cytotoxic antibodies CDC crossmatching Donor-specific antigen crossmatch Health sciences theses SDG-03 Health sciences theses SDG-10 Health sciences theses SDG-17 Thesis (PhD)--University of Pretoria, 2018. The study addressed the development and evaluation of novel procedures for the rapid, reproducible and efficient measurement of T cell alloreactivity both pre- and post-transplantation, with the aim of optimizing donor/ recipient compatibility in renal transplantation. Furthermore, a comparison of the three currently available procedures for the detection of cytotoxic antibodies to determine which of these is best suited to optimizing pre-transplantation donor-recipient matching, as well as post-transplantation detection of sensitization to donor alloantigens. She established that the standard CDC crossmatching (CDCXM) had a high false-positive rate and that the Donor specific antigen crossmatch (DSAXM) method delivered the most accurate results for identifying anti-HLA antibodies in prospective, pre-sensitised kidney transplant patients. Cytokine profile analysis identified the involvement of eosinophils and a marked increase of anti-inflammatory cytokines that may possibly contribute to the initiation of kidney transplant rejection. Finally, she has shown that, the use of novel, CFSE-based two-way mixed lymphocyte cultures (MLC) adapted for flow cytometry may be able to identify patients that are at risk of transplant rejection. Furthermore, this novel method may possibly be the test of choice to indicate probable graft-versus-host-disease (GVHD) incidences. The overall findings of the study may contribute to optimising histocompatibility testing in renal transplantation by improving HLA matching criteria as well as identifying potential strategies to monitor and prevent GVHD. National Health Laboratory Services Research Trust em2025 Immunology PhD Unrestricted SDG-03: Good health and well-being SDG-10: Reduced inequalities SDG-17: Partnerships for the goals 2019-02-14T12:51:10Z 2019-02-14T12:51:10Z 2019-04 2018 Thesis Kwofie, LLI 2018, Optimisation of histocompatibility testing in living-donor renal transplantation, PhD Thesis, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/68460> A2019 http://hdl.handle.net/2263/68460 en © 2018 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | HLA cross-match Histocompatibility End-stage kidney diease Allotransplantation Alloantigen T cell alloreactivity Renal transplantation Donor-recipient compatibility Kidney transplant Cytotoxic antibodies CDC crossmatching Donor-specific antigen crossmatch Health sciences theses SDG-03 Health sciences theses SDG-10 Health sciences theses SDG-17 Optimisation of histocompatibility testing in living-donor renal transplantation |
| title | Optimisation of histocompatibility testing in living-donor renal transplantation |
| title_full | Optimisation of histocompatibility testing in living-donor renal transplantation |
| title_fullStr | Optimisation of histocompatibility testing in living-donor renal transplantation |
| title_full_unstemmed | Optimisation of histocompatibility testing in living-donor renal transplantation |
| title_short | Optimisation of histocompatibility testing in living-donor renal transplantation |
| title_sort | optimisation of histocompatibility testing in living donor renal transplantation |
| topic | HLA cross-match Histocompatibility End-stage kidney diease Allotransplantation Alloantigen T cell alloreactivity Renal transplantation Donor-recipient compatibility Kidney transplant Cytotoxic antibodies CDC crossmatching Donor-specific antigen crossmatch Health sciences theses SDG-03 Health sciences theses SDG-10 Health sciences theses SDG-17 |
| url | http://hdl.handle.net/2263/68460 |