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Spatial characterization of the BT-20 triple negative breast cancer spheroidal model
Dissertation (MSc)--University of Pretoria, 2019.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2019
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| _version_ | 1867613608877228032 |
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| access_status_str | Open Access |
| author2 | Cordier, Werner |
| author_browse | Cordier, Werner |
| author_facet | Cordier, Werner |
| collection | Thesis |
| dc_rights_str_mv | © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2019. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/70368 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:38:51.633Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/70368 Spatial characterization of the BT-20 triple negative breast cancer spheroidal model Cordier, Werner keith07ncube@gmail.com Steenkamp, Vanessa Cromarty, Allan Duncan Van den Bout, Jan Iman Ncube, Keith Ntokozo UCTD Health sciences theses SDG-03 Health sciences theses SDG-17 Dissertation (MSc)--University of Pretoria, 2019. Breast cancer is a commonly diagnosed cancer in women, with increasing diagnosis and mortality rates. The triple-negative sub-type of the disease is characterised by lack of hormone-receptor overexpression, exhibition of poorly characterised molecular aberrations, and treatment failure. Chemotherapy is the current mainstay; however, it fails to slow tumor progression, and this is partially attributed to the lack of characterisation of biological features that drive treatment failure. There is, therefore, a need to characterise the biological features of triple-negative breast cancer, in order to develop effective therapies against the disease. Researchers widely use monolayer cell culture in pre-clinical screening of anticancer drugs. However, the development of effective anti-cancer drugs is hampered by limitations inherent to these culture systems, as they insufficiently mimic the physiological characteristics of tumours in vivo. Spheroids have been suggested as a bridge to the gap between monolayers and animal models, as they combine the flexibility and cost-effectiveness of cell culture with the spatial and molecular attributes of tissue. This study aimed to grow and characterise a spheroid model of triple-negative breast cancer with regards to growth, morphology, and drug sensitivity. The hanging drop and liquid overlay techniques were compared to select a method for growing spheroids. Spheroid growth was assessed using phase contrast microscopy and the bicinchoninic acid assay. Viability was assessed using the fluorescein diacetate (FDA)/propidium iodide (PI) assay. Haematoxylin and eosin staining were used for morphological evaluation. An iridium complex was used to investigate the induction of hypoxia. The sulphorodamine B and acid phosphatase assays, FDA/PI staining and phase contrast microscopy were used to assess the 72-h cytotoxicity effects of doxorubicin in monolayers and spheroids. Immunostaining and optical clearing were used to visualise the spatial distribution of the Ki-67 antigen and cadherins in spheroids. National Research Foundation University of Pretoria em2026 Pharmacology MSc Unrestricted SDG-03: Good health and well-being SDG-17: Partnerships for the goals 2019-07-04T12:14:45Z 2019-07-04T12:14:45Z 2019-09 2019 Dissertation Ncube, KN 2019, Spatial characterization of the BT-20 triple negative breast cancer spheroidal model, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/70368> S2019 http://hdl.handle.net/2263/70368 en © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Health sciences theses SDG-03 Health sciences theses SDG-17 Spatial characterization of the BT-20 triple negative breast cancer spheroidal model |
| title | Spatial characterization of the BT-20 triple negative breast cancer spheroidal model |
| title_full | Spatial characterization of the BT-20 triple negative breast cancer spheroidal model |
| title_fullStr | Spatial characterization of the BT-20 triple negative breast cancer spheroidal model |
| title_full_unstemmed | Spatial characterization of the BT-20 triple negative breast cancer spheroidal model |
| title_short | Spatial characterization of the BT-20 triple negative breast cancer spheroidal model |
| title_sort | spatial characterization of the bt 20 triple negative breast cancer spheroidal model |
| topic | UCTD Health sciences theses SDG-03 Health sciences theses SDG-17 |
| url | http://hdl.handle.net/2263/70368 |