Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Genomic comparison of mycoplasma species isolated from commercial chickens in South Africa
Thesis (PhD)--University of Pretoria, 2018.
Saved in:
| Other Authors: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
University of Pretoria
2019
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613727399870464 |
|---|---|
| access_status_str | Open Access |
| author2 | Abolnik, Celia |
| author_browse | Abolnik, Celia |
| author_facet | Abolnik, Celia |
| collection | Thesis |
| dc_rights_str_mv | © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Thesis (PhD)--University of Pretoria, 2018. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/70444 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:40:44.617Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/70444 Genomic comparison of mycoplasma species isolated from commercial chickens in South Africa Abolnik, Celia u25285573@tuks.co.za Beylefeld, Amanda UCTD Veterinary science theses SDG-02 Veterinary science theses SDG-03 Veterinary science theses SDG-09 Thesis (PhD)--University of Pretoria, 2018. Mycoplasma gallisepticum and Mycoplasma synoviae are listed by the World Organisation for Animal Health (OIE) as notifiable avian diseases agents and are considered as economically important species affecting the South African poultry industry. A decreased number of cases identified by culture with growth inhibition of M. gallisepticum and M. synoviae and an increase in unidentified mycoplasma species were observed at the University of Pretoria diagnostic laboratory. Samples were isolated from chickens displaying typical signs associated with pathogenic mycoplasma infection, thus necessitating a closer look at the lesser known mycoplasma species found in poultry. The aim of this study was to use second generation sequencing to identify and compare mycoplasma species isolated from commercial chickens in South Africa. Mycoplasma samples were isolated, sequenced and de novo assembled for identification by 16S rRNA phylogeny. A total of 124 samples were received between 2003 and 2015, 44 of these samples contained multiple species resulting in 179 isolates identified as M. gallisepticum (24.58%), M. synoviae (9.50%), M. gallinaceum (24.02%), M. gallinarum (24.58%), M. pullorum (13.97%) and M. iners (2.79%) and one Acholeplasma laidlawii (0.56%). Antimicrobial resistance (AMR) to chlortetracycline, enrofloxacin, tylosin and tiamulin and the genes involved in AMR were studied. Three M. gallinaceum samples showed possible multidrug resistance and novel point mutations associated with AMR in M. gallinaceum and M. gallinarum were identified. The first complete genome of M. pullorum was assembled, annotated and published. Draft genome assemblies for axenic strains were constructed and candidate genes that can be tested for novel diagnostic and vaccine targets were identified. es2025 Production Animal Studies PhD Unrestricted SDG-02: Zero hunger SDG-03: Good health and well-being SDG-09: Industry, innovation and infrastructure 2019-07-08T09:46:26Z 2019-07-08T09:46:26Z 2019/04/04 2018 Thesis Beylefeld, A 2018, Genomic comparison of mycoplasma species isolated from commercial chickens in South Africa, PhD Thesis, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/70444> A2019 http://hdl.handle.net/2263/70444 en © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Veterinary science theses SDG-02 Veterinary science theses SDG-03 Veterinary science theses SDG-09 Genomic comparison of mycoplasma species isolated from commercial chickens in South Africa |
| title | Genomic comparison of mycoplasma species isolated from commercial chickens in South Africa |
| title_full | Genomic comparison of mycoplasma species isolated from commercial chickens in South Africa |
| title_fullStr | Genomic comparison of mycoplasma species isolated from commercial chickens in South Africa |
| title_full_unstemmed | Genomic comparison of mycoplasma species isolated from commercial chickens in South Africa |
| title_short | Genomic comparison of mycoplasma species isolated from commercial chickens in South Africa |
| title_sort | genomic comparison of mycoplasma species isolated from commercial chickens in south africa |
| topic | UCTD Veterinary science theses SDG-02 Veterinary science theses SDG-03 Veterinary science theses SDG-09 |
| url | http://hdl.handle.net/2263/70444 |