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Molecular detection of acaricide resistance in Rhipicephalus microplus in Engcobo Local Municipality Eastern Cape South Africa
Dissertation (MSc)--University of Pretoria, 2018.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2019
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| _version_ | 1867613593804996608 |
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| access_status_str | Open Access |
| author2 | Crafford, Jan Ernst |
| author_browse | Crafford, Jan Ernst |
| author_facet | Crafford, Jan Ernst |
| collection | Thesis |
| dc_rights_str_mv | © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2018. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/70546 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:38:36.983Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/70546 Molecular detection of acaricide resistance in Rhipicephalus microplus in Engcobo Local Municipality Eastern Cape South Africa Crafford, Jan Ernst u16391196@tuks.co.za Maritz-Olivier, Christine Zanga, Jaison UCTD Veterinary science theses SDG-01 SDG-01: No poverty Dissertation (MSc)--University of Pretoria, 2018. Rhipicephalus microplus transmit two important diseases of livestock in South Africa, namely Babesiosis and Anaplasmosis. As cattle still play a key role in the livelihood of rural communities in the Eastern Cape province, animal health is of utmost importance to maintain and strengthen these communities. In most rural areas of the Eastern Cape, the South African government provides free dipping of cattle with amitraz. Deltamethrin, a synthetic pyrethroid (SP), has been used as pour-on dip in a few locations where there are no functional dips. Of concern, is the growing number of global reports on the increase of acaricide resistance. Routine screening for resistance to acaricides is therefore needed. In this study we investigated allele frequencies of acaricide resistance-associated single nucleotide polymorphisms (SNPs) in R. microplus ticks from the Engcobo Local Municipality. The estimated frequencies of amitraz resistance-associated SNPs were 0.58 (at locus 1) and 0.32 (at locus 3). Resistance against formamidines (amitraz) appears to be on the rise. The published mutation in the voltage gated sodium channel (VGS) receptor gene (domain II segment 4-5 region), known to confer resistance to synthetic pyrethroids, was not found. This could be attributed to the low selection pressure against pyrethroids in the study area. Veterinary Tropical Diseases MSc Unrestricted 2019-07-08T09:46:50Z 2019-07-08T09:46:50Z 2019/04/04 2018 Dissertation Zanga, J 2018, Molecular detection of acaricide resistance in Rhipicephalus microplus in Engcobo Local Municipality Eastern Cape South Africa, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/70546> A2019 http://hdl.handle.net/2263/70546 en © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Veterinary science theses SDG-01 SDG-01: No poverty Molecular detection of acaricide resistance in Rhipicephalus microplus in Engcobo Local Municipality Eastern Cape South Africa |
| title | Molecular detection of acaricide resistance in Rhipicephalus microplus in Engcobo Local Municipality Eastern Cape South Africa |
| title_full | Molecular detection of acaricide resistance in Rhipicephalus microplus in Engcobo Local Municipality Eastern Cape South Africa |
| title_fullStr | Molecular detection of acaricide resistance in Rhipicephalus microplus in Engcobo Local Municipality Eastern Cape South Africa |
| title_full_unstemmed | Molecular detection of acaricide resistance in Rhipicephalus microplus in Engcobo Local Municipality Eastern Cape South Africa |
| title_short | Molecular detection of acaricide resistance in Rhipicephalus microplus in Engcobo Local Municipality Eastern Cape South Africa |
| title_sort | molecular detection of acaricide resistance in rhipicephalus microplus in engcobo local municipality eastern cape south africa |
| topic | UCTD Veterinary science theses SDG-01 SDG-01: No poverty |
| url | http://hdl.handle.net/2263/70546 |