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In vitro cytotoxic effects and ultrastructural changes in myocardial (H9c2) and neuroblastoma (neuro-2a) cell lines following exposure to three different cardiac glycosides
Dissertation (MSc)--University of Pretoria, 2018.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2019
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| _version_ | 1867613565507076096 |
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| access_status_str | Open Access |
| author2 | Botha, C.J. (Christoffel Jacobus) |
| author_browse | Botha, C.J. (Christoffel Jacobus) |
| author_facet | Botha, C.J. (Christoffel Jacobus) |
| collection | Thesis |
| dc_rights_str_mv | © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2018. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/70585 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:38:09.710Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/70585 In vitro cytotoxic effects and ultrastructural changes in myocardial (H9c2) and neuroblastoma (neuro-2a) cell lines following exposure to three different cardiac glycosides Botha, C.J. (Christoffel Jacobus) u113009410@tuks.co.za Henn, Danielle UCTD Cardiac glycosides Cytotoxicity In vitro toxicity Cardiac cells Apoptosis Necrosis Veterinary science theses SDG-03 Veterinary science theses SDG-09 Veterinary science theses SDG-12 Dissertation (MSc)--University of Pretoria, 2018. Cardiac glycosides are often found within plants and can be classified as either cardenolides or bufadienolides depending on their structure. Cardenolide-containing plants are seldom eaten by livestock and of little veterinary importance. Comparatively, bufadienolide-containing plants are eaten much more often and cause a significant number of livestock mortalities. The compounds classified as bufadienolides can further be divided into two groups i.e. non-cumulative bufadienolides, which cause acute bufadienolide poisoning, and cumulative bufadienolides, that beside acute poisoning, also cause chronic intoxication. The chronic form of bufadienolide induced poisoning is a paretic condition known as krimpsiekte that mainly affect small stock. Our study objectives of this project were to confirm the neurotoxicity of the cumulative bufadienolides in vitro and compare the effects of different types of cardiac glycosides on myocardial and neuroblastoma cell lines. The in vitro cytotoxicity of the cardenolide digoxin, the non-cumulative bufadienolide 1α,2α-epoxyscillirosidine and the cumulative bufadienolide lanceotoxin B on H9c2 and Neuro-2a cells were determined using the MTT assay. The cytotoxicity of 1α,2α-epoxyscillirosidine on H9c2 cells was the greatest of the three cardiac glycosides tested. In contrast, Neuro-2a cells suffered the highest degree of cytotoxicity when exposed to lanceotoxin B. Ultrastructural changes induced by the different cardiac glycosides were examined using electron microscopy. The morphological changes induced by the cardiac glycosides was used to indicate the possible mechanism of cell death. The majority of H9c2 cells exposed to digoxin and 1α,2α-epoxyscillirosidine died via necrosis, while H9c2 cells exposed to lanceotoxin B died via apoptosis. The Neuro-2a cells exposed to digoxin showed signs of dying via apoptosis. 1α,2α-Epoxyscillirosidine caused necrosis in Neuro-2a cells, with some cells dying via the apoptotic pathway. Finally, lanceotoxin B caused many cells to exhibit the hallmark features of apoptosis, but the large autophagic vesicles present within the cytoplasm could be indicative of the involvement of autophagy in cell death. es2026 Paraclinical Sciences MSc Unrestricted SDG-03: Good health and well-being SDG-09: Industry, innovation and infrastructure SDG-12: Responsible consumption and production 2019-07-08T09:46:56Z 2019-07-08T09:46:56Z 2019/04/04 2018 Dissertation Henn, D 2018, In vitro cytotoxic effects and ultrastructural changes in myocardial (H9c2) and neuroblastoma (neuro-2a) cell lines following exposure to three different cardiac glycosides, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/70585> A2019 http://hdl.handle.net/2263/70585 en © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Cardiac glycosides Cytotoxicity In vitro toxicity Cardiac cells Apoptosis Necrosis Veterinary science theses SDG-03 Veterinary science theses SDG-09 Veterinary science theses SDG-12 In vitro cytotoxic effects and ultrastructural changes in myocardial (H9c2) and neuroblastoma (neuro-2a) cell lines following exposure to three different cardiac glycosides |
| title | In vitro cytotoxic effects and ultrastructural changes in myocardial (H9c2) and neuroblastoma (neuro-2a) cell lines following exposure to three different cardiac glycosides |
| title_full | In vitro cytotoxic effects and ultrastructural changes in myocardial (H9c2) and neuroblastoma (neuro-2a) cell lines following exposure to three different cardiac glycosides |
| title_fullStr | In vitro cytotoxic effects and ultrastructural changes in myocardial (H9c2) and neuroblastoma (neuro-2a) cell lines following exposure to three different cardiac glycosides |
| title_full_unstemmed | In vitro cytotoxic effects and ultrastructural changes in myocardial (H9c2) and neuroblastoma (neuro-2a) cell lines following exposure to three different cardiac glycosides |
| title_short | In vitro cytotoxic effects and ultrastructural changes in myocardial (H9c2) and neuroblastoma (neuro-2a) cell lines following exposure to three different cardiac glycosides |
| title_sort | in vitro cytotoxic effects and ultrastructural changes in myocardial h9c2 and neuroblastoma neuro 2a cell lines following exposure to three different cardiac glycosides |
| topic | UCTD Cardiac glycosides Cytotoxicity In vitro toxicity Cardiac cells Apoptosis Necrosis Veterinary science theses SDG-03 Veterinary science theses SDG-09 Veterinary science theses SDG-12 |
| url | http://hdl.handle.net/2263/70585 |