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The pathology of the spleen in canine babesiosis
Dissertation (M.Med.Vet)--University of Pretoria, 2019.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2019
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| _version_ | 1867613660176711680 |
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| access_status_str | Open Access |
| author2 | Leisewitz, Andrew L. |
| author_browse | Leisewitz, Andrew L. |
| author_facet | Leisewitz, Andrew L. |
| collection | Thesis |
| dc_rights_str_mv | © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (M.Med.Vet)--University of Pretoria, 2019. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/70587 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:39:40.578Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/70587 The pathology of the spleen in canine babesiosis Leisewitz, Andrew L. u24054781@tuks.co.za Clift, Sarah J. Henning, Alischa UCTD Canine babesiosis Tick-borne Parasitic infection Lymphoid organ Infected spleens Veterinary science theses SDG-02 Veterinary science theses SDG-03 Veterinary science theses SDG-15 Dissertation (M.Med.Vet)--University of Pretoria, 2019. The spleen is the largest secondary lymphoid organ in the body and therefore not only does it perform many hematopoietic functions but it also mounts an immunological response to blood-borne antigens. Malaria and babesiosis, both haemoparasitic diseases, reveal many similarities in clinical disease, pathogenesis and post mortem findings and are often compared to one another. Both are protozoan diseases transmitted by mosquitoes and ticks, respectively. Malaria has been more extensively studied than babesiosis. Information regarding the effect of babesiosis on various hosts is limited, particularly with respect to the spleen. The purpose of this study was to investigate the effect of Babesia rossi on the spleen of dogs. We aimed to provide a detailed histomorphological analysis of infected spleens, with the addition of immunohistochemical labelling of leukocyte subsets. One section of splenic tissue from each of 9 Babesia rossi-infected dogs and 4 healthy control dogs was examined under the light microscope. Immunohistochemical markers were applied to infected and control spleens in order to characterise different immunocyte populations. Markers included CD3 (T lymphocytes), CD20 (mature B lymphocytes and normal dog plasma cells), Mum1 (plasma cells), Pax-5 (immature B lymphocytes), Mac387 (monocytes-macrophages of bone marrow origin) and CD204 (resident tissue macrophages). The application of analytic software enabled us to compare leukocyte subpopulations in infected and control spleens in a semi-quantitative manner. Routine histopathology revealed diffuse intermingling of the white and red pulp in Babesia rossi-infected spleens with a clear loss of distinction between these zones. The merging of zones was accentuated by outspoken white pulp dissolution with no discernible germinal centres, mantle or marginal zones. Immunohistochemical labelling revealed a significant increase in the proportion of tissue resident macrophages as well as macrophages of bone marrow origin in the infected spleens. In addition, apart from a few remnant lymphocytes within the peri-arteriolar lymphatic sheaths and follicles, the majority of immunocytes had redistributed to the red pulp, supporting the observation of white and red pulp intermingling. Our study produced novel insights into the pathology of the spleen in canine babesiosis. The majority of our findings are in agreement with histomorphological descriptions of the spleen in a variety of hosts with malaria. The exact causes, consequences and implications for disease pathogenesis require further investigation. es2026 Paraclinical Sciences M.Med.Vet Unrestricted SDG-02: Zero hunger SDG-03: Good health and well-being SDG-15: Life on land 2019-07-08T09:46:56Z 2019-07-08T09:46:56Z 2019/04/04 2019 Dissertation Henning, A 2019, The pathology of the spleen in canine babesiosis, M.Med.Vet Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/70587> A2019 http://hdl.handle.net/2263/70587 en © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Canine babesiosis Tick-borne Parasitic infection Lymphoid organ Infected spleens Veterinary science theses SDG-02 Veterinary science theses SDG-03 Veterinary science theses SDG-15 The pathology of the spleen in canine babesiosis |
| title | The pathology of the spleen in canine babesiosis |
| title_full | The pathology of the spleen in canine babesiosis |
| title_fullStr | The pathology of the spleen in canine babesiosis |
| title_full_unstemmed | The pathology of the spleen in canine babesiosis |
| title_short | The pathology of the spleen in canine babesiosis |
| title_sort | pathology of the spleen in canine babesiosis |
| topic | UCTD Canine babesiosis Tick-borne Parasitic infection Lymphoid organ Infected spleens Veterinary science theses SDG-02 Veterinary science theses SDG-03 Veterinary science theses SDG-15 |
| url | http://hdl.handle.net/2263/70587 |