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Evaluation of simple sequence repeats (SSR) markers associated with resistance to Sclerotinia sclerotiorum (Lib.) de Barry in commercial South African soybean cultivars
Dissertation (MSc)--University of Pretoria, 2019.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2019
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| _version_ | 1867613622220357632 |
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| access_status_str | Open Access |
| author2 | Vorster, Barend Juan |
| author_browse | Vorster, Barend Juan |
| author_facet | Vorster, Barend Juan |
| collection | Thesis |
| dc_rights_str_mv | © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2019. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/72802 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:39:04.068Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/72802 Evaluation of simple sequence repeats (SSR) markers associated with resistance to Sclerotinia sclerotiorum (Lib.) de Barry in commercial South African soybean cultivars Vorster, Barend Juan u11206111@tuks.co.za Van der Waals, Jacqueline Elise Mbedzi, Pfano Princess UCTD Dissertation (MSc)--University of Pretoria, 2019. Sclerotinia sclerotiorum (Lib.) de Barry, commonly known as Sclerotinia stem rot is one of the pathogens that have a potentially devastating impact on the growth of soybean industry in South Africa. Several quantitative trait loci (QTL) that play a role in soybean resistance to Sclerotinia stem rot have been identified and mapped on the soybean’s integrated genetic linkage map. However, none of these QTL and their underlying markers have been evaluated in any of the commercial soybean cultivars grown in South Africa. The aim of this study was to screen and characterise SSR markers linked to QTL that are reported to have an association with stem rot resistance on South African commercial soybean [Glycine max (L.) Merr] cultivars. Twenty SSR markers that have been reported to have an association with stem rot resistance on soybean were evaluated in 29 commercial soybean cultivars that are currently planted in South Africa. The genetic diversity of markers and the genetic relationships across the cultivars were evaluated using the GenAlEX software package. Five markers were not polymorphic, while twelve markers were polymorphic across all cultivars. Evaluation of genetic relationships across cultivars showed that 10 South African cultivars share alleles with a cultivar with known resistance (M.A) to Sclerotinia stem rot. We propose that the shared alleles between 10 South African cultivars and M.A could indicate shared resistance to Sclerotinia stem rot and indicates the presence of a genetic rather than environmental mechanism of resistance in South African soybean cultivars. The results from this study will aid South African soybean breeders in knowing which markers they can use to screen for Sclerotinia stem rot resistance within the context of South African soybean cultivars. Plant Production and Soil Science MSc Unrestricted 2019-12-13T08:08:18Z 2019-12-13T08:08:18Z 2019/09/05 2019 Dissertation Mbedzi, PP 2019, Evaluation of simple sequence repeats (SSR) markers associated with resistance to Sclerotinia sclerotiorum (Lib.) de Barry in commercial South African soybean cultivars, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/72802> S2019 http://hdl.handle.net/2263/72802 en © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Evaluation of simple sequence repeats (SSR) markers associated with resistance to Sclerotinia sclerotiorum (Lib.) de Barry in commercial South African soybean cultivars |
| title | Evaluation of simple sequence repeats (SSR) markers associated with resistance to Sclerotinia sclerotiorum (Lib.) de Barry in commercial South African soybean cultivars |
| title_full | Evaluation of simple sequence repeats (SSR) markers associated with resistance to Sclerotinia sclerotiorum (Lib.) de Barry in commercial South African soybean cultivars |
| title_fullStr | Evaluation of simple sequence repeats (SSR) markers associated with resistance to Sclerotinia sclerotiorum (Lib.) de Barry in commercial South African soybean cultivars |
| title_full_unstemmed | Evaluation of simple sequence repeats (SSR) markers associated with resistance to Sclerotinia sclerotiorum (Lib.) de Barry in commercial South African soybean cultivars |
| title_short | Evaluation of simple sequence repeats (SSR) markers associated with resistance to Sclerotinia sclerotiorum (Lib.) de Barry in commercial South African soybean cultivars |
| title_sort | evaluation of simple sequence repeats ssr markers associated with resistance to sclerotinia sclerotiorum lib de barry in commercial south african soybean cultivars |
| topic | UCTD |
| url | http://hdl.handle.net/2263/72802 |