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Assessment of in vivo calcium and phosphorus digestibility in commercial laying hens fed limestone with different particle sizes

Dissertation (MSc Agric (Animal Science: Animal Nutrition))--University of Pretoria, 2020

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Other Authors: Nkukwana, Thobela T.
Format: Thesis
Language:English
Published: University of Pretoria 2020
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access_status_str Open Access
author2 Nkukwana, Thobela T.
author_browse Nkukwana, Thobela T.
author_facet Nkukwana, Thobela T.
collection Thesis
dc_rights_str_mv © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.
description Dissertation (MSc Agric (Animal Science: Animal Nutrition))--University of Pretoria, 2020
format Thesis
id oai:repository.up.ac.za:2263/73770
institution University of Pretoria (South Africa)
language English
last_indexed 2026-06-10T12:38:02.052Z
license_str Other — see source repository
provenance_str_mv Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository
publishDate 2020
publishDateRange 2020
publishDateSort 2020
publisher University of Pretoria
publisherStr University of Pretoria
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source_str UPSpace — University of Pretoria Institutional Repository
spelling oai:repository.up.ac.za:2263/73770 Assessment of in vivo calcium and phosphorus digestibility in commercial laying hens fed limestone with different particle sizes Nkukwana, Thobela T. msinclairblack@gmail.com Plumstead, Peter Sinclair-Black, Micaela UCTD Poultry Nutrition Calcium digestibility Phosphorus digestibility Commercial laying hens Limestone particle size Particle size distribution Calcium source Eggshell quality Natural and agricultural sciences theses SDG-02 Natural and agricultural sciences theses SDG-12 Natural and agricultural sciences theses SDG-15 Dissertation (MSc Agric (Animal Science: Animal Nutrition))--University of Pretoria, 2020 Ascertaining calcium (Ca) and phosphorus (P) digestibility values remain a challenge in the determination of nutrient requirements of laying hens. In recent years, limited research has been conducted in this area, and there is scope to update literature to reflect the requirements of the modern hen. The present study investigated the effects of time post-oviposition (POP), limestone particle size (PS) and phytase inclusion on Ca and P digestibility, blood ionized calcium (iCa), and eggshell percentage (PE) in commercial laying hens. The treatments were comprised of two levels of phytase (0, 600 phytase units (FTU)) and two particle sizes of limestone (0.25 mm and 1.5 mm) to yield a 2x2 factorial experimental design. Digestibility values were determined at 3h and 11h POP and blood iCa values were determined at (0, 3, 6, 9, 12, 15, 18, 21) hours POP. Ca digestibility was significantly affected by time POP (P<0.01), with a higher Ca digestibility being noted at 11 hours POP during eggshell calcification than at 3 hours POP, during the inactive phase of the shell gland. There was no significant effect of limestone PS and phytase on Ca digestibility (P >0.5). However, there was a two-way interaction between the limestone PS and phytase (P<0.05). Blood iCa was also significantly affected by the time POP. Blood iCa remained high during the shell gland inactivity period and decreased rapidly during the period of eggshell calcification (P<0.01). Blood iCa was also affected by a two-way interaction between limestone PS and phytase. A significant effect of limestone PS on PE was noted, with limestone grit providing an improved PE (P<0.01). P digestibility was significantly improved by the addition of 600 FTU phytase (P<0.001). The time POP affected P digestibility (P<0.01) with P digestibility being greater at 3h POP than 11h POP. The results from this trial illustrate the presence of diurnal circadian rhythm for Ca digestibility and P digestibility. The results from the blood iCa and PE demonstrate that limestone grit improves the Ca status of the hen and that the combination of fine limestone and the addition of phytase enzyme had a positive effect on Ca and P digestibility. DuPont Animal Sciences bs2026 Animal and Wildlife Sciences Msc Agric (Animal Science: Animal Nutrition) Unrestricted SDG-02: Zero hunger SDG-12: Responsible consumption and production SDG-15: Life on land 2020-03-17T06:51:40Z 2020-03-17T06:51:40Z 2020-09-20 2020 Dissertation * S2019 http://hdl.handle.net/2263/73770 en © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria
spellingShingle UCTD
Poultry Nutrition
Calcium digestibility
Phosphorus digestibility
Commercial laying hens
Limestone particle size
Particle size distribution
Calcium source
Eggshell quality
Natural and agricultural sciences theses SDG-02
Natural and agricultural sciences theses SDG-12
Natural and agricultural sciences theses SDG-15
Assessment of in vivo calcium and phosphorus digestibility in commercial laying hens fed limestone with different particle sizes
title Assessment of in vivo calcium and phosphorus digestibility in commercial laying hens fed limestone with different particle sizes
title_full Assessment of in vivo calcium and phosphorus digestibility in commercial laying hens fed limestone with different particle sizes
title_fullStr Assessment of in vivo calcium and phosphorus digestibility in commercial laying hens fed limestone with different particle sizes
title_full_unstemmed Assessment of in vivo calcium and phosphorus digestibility in commercial laying hens fed limestone with different particle sizes
title_short Assessment of in vivo calcium and phosphorus digestibility in commercial laying hens fed limestone with different particle sizes
title_sort assessment of in vivo calcium and phosphorus digestibility in commercial laying hens fed limestone with different particle sizes
topic UCTD
Poultry Nutrition
Calcium digestibility
Phosphorus digestibility
Commercial laying hens
Limestone particle size
Particle size distribution
Calcium source
Eggshell quality
Natural and agricultural sciences theses SDG-02
Natural and agricultural sciences theses SDG-12
Natural and agricultural sciences theses SDG-15
url http://hdl.handle.net/2263/73770