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The effects of medicinal plant extracts on the microbial, biofilm and autolytic activity of several pathogenic bacteria.
Dissertation (MSc)--University of Pretoria, 2018.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2020
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| _version_ | 1867613558000320512 |
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| access_status_str | Open Access |
| author2 | Beukes, Mervyn |
| author_browse | Beukes, Mervyn |
| author_facet | Beukes, Mervyn |
| collection | Thesis |
| dc_rights_str_mv | © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2018. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/74851 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:38:02.940Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/74851 The effects of medicinal plant extracts on the microbial, biofilm and autolytic activity of several pathogenic bacteria. Beukes, Mervyn u12022927@tuks.co.za Demetriou, Demetra Danielle Biochemistry UCTD Dissertation (MSc)--University of Pretoria, 2018. More people are aware of the problems with drugs and are reverting to herbal (plant) and over-the-counter medications. The conclusion made is the urgency to study new plants for antimicrobial activity. Various plant extracts were used in the study to determine the antimicrobial, mode of action and autolytic activity against various microbial species. Techniques used in this study include culturing via streaking method, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), zymography, high performance liquid chromatography (HPLC), autolytic activity assay, minimum inhibition concentration assay, dilution activity assay, Spot-on-lawn assay and Bicinchoninic acid (BCA) assay. The whole cell protein profile was used to show the proteins without any extraction method for comparison with the applied extraction method. With the extraction method implemented, less proteins where seen as the extraction method was specific to the peptidoglycan hydrolases. Sodium dodecyl sulfate (SDS) (4%) extraction method was more effective as an extraction method when compared to lithium chloride (LiCl). Plant extract 4.2 showedantimicrobial activity as seen by the inhibition zone on the agar plate (Figure 27 and Figure 28). Plant extract 4.2 and 5.2 showed decreased autolytic activity (Figure 20-22). The conclusion made was that the presence of the plant extracts decreases growth of the microbial species thus decreases protein production. Markers were identified using HPLC where 1 marker showed antimicrobial activity (Sebacic acid) and another marker showed anti-inflammatory activity (Loxoprofen Sodium) based on previous literature. S. aureus 12600 in the presences of extract 4.2 had antimicrobial activity but results were not significant. Extract 5.2 showed biofilm formation. Extract 4.2 showed positive results in most experiments conducted. South African Biochemistry and Informatics for Natural Products (SABINA) National Research Foundation (NRF) Biochemistry MSc Unrestricted 2020-06-03T12:42:47Z 2020-06-03T12:42:47Z 2020-04 2018 Dissertation Demetriou, DD 2018, The effects of medicinal plant extracts on the microbial, biofilm and autolytic activity of several pathogenic bacteria., MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/74851> http://hdl.handle.net/2263/74851 en © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Biochemistry UCTD The effects of medicinal plant extracts on the microbial, biofilm and autolytic activity of several pathogenic bacteria. |
| title | The effects of medicinal plant extracts on the microbial, biofilm and autolytic activity of several pathogenic bacteria. |
| title_full | The effects of medicinal plant extracts on the microbial, biofilm and autolytic activity of several pathogenic bacteria. |
| title_fullStr | The effects of medicinal plant extracts on the microbial, biofilm and autolytic activity of several pathogenic bacteria. |
| title_full_unstemmed | The effects of medicinal plant extracts on the microbial, biofilm and autolytic activity of several pathogenic bacteria. |
| title_short | The effects of medicinal plant extracts on the microbial, biofilm and autolytic activity of several pathogenic bacteria. |
| title_sort | effects of medicinal plant extracts on the microbial biofilm and autolytic activity of several pathogenic bacteria |
| topic | Biochemistry UCTD |
| url | http://hdl.handle.net/2263/74851 |